VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction
Inducible regulatory T cells (Tregs) exert a timely and efficient immunosuppressive action at the critical peri-implantation stage essential for maternal tolerance to the conceptus. Vasoactive intestinal peptide (VIP) promotes anti-inflammatory and tolerogenic profiles through binding to VIP recepto...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v98_n1_p49_Fraccaroli http://hdl.handle.net/20.500.12110/paper_07415400_v98_n1_p49_Fraccaroli |
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paper:paper_07415400_v98_n1_p49_Fraccaroli2023-06-08T15:44:40Z VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction Early pregnancy iTreg TGF-β Tolerance CD4 antigen gamma interferon interleukin 10 interleukin 17 polypeptide transcription factor FOXP3 transforming growth factor beta transforming growth factor beta antibody vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor interleukin 10 transforming growth factor beta1 vasoactive intestinal polypeptide Article cell interaction cell population cell suspension coculture controlled study cytokine production DNA cross linking DNA synthesis female first trimester pregnancy human human cell immune response in vitro study intracellular space leukocyte peripheral blood mononuclear cell priority journal regulatory T lymphocyte trophoblast biosynthesis cell communication cell line cytology drug effects immunology leukocyte metabolism pregnancy real time polymerase chain reaction regulatory T lymphocyte trophoblast Cell Communication Cell Line Coculture Techniques Female Humans In Vitro Techniques Interleukin-10 Leukocytes Pregnancy Pregnancy Trimester, First Real-Time Polymerase Chain Reaction T-Lymphocytes, Regulatory Transforming Growth Factor beta1 Trophoblasts Vasoactive Intestinal Peptide Inducible regulatory T cells (Tregs) exert a timely and efficient immunosuppressive action at the critical peri-implantation stage essential for maternal tolerance to the conceptus. Vasoactive intestinal peptide (VIP) promotes anti-inflammatory and tolerogenic profiles through binding to VIP receptors on immune cells. We evaluated whether VIP produced by trophoblast cells induces Tregs during the early interaction of maternal leukocytes with trophoblast cells, thus contributing to maternal tolerance. We used an in vitro model of maternal leukocyte–trophoblast cell interaction represented by cocultures of fertile women’s PBMCs with a human trophoblast cell line (Swan-71) and evaluated the effect of VIP added exogenously and of the endogenous polypeptide. VIP increased the frequency of CD4+CD25+FoxP3+ cells after coculture, and these cells were able to suppress the maternal alloresponse. VIP also increased the frequency of CD4+IL10+ and CD4+TGFβ+ cells, but it did not modulate IFN-γ or IL-17 production. Swan-71 secreted VIP, and their coculture with maternal PBMCs significantly increased the frequency of Tregs. This effect was even more pronounced if the trophoblast cells had been pretreated with VIP. In both situations, the VIP antagonist prevented the increase in the frequency of CD4+Foxp3+ cells, reflecting a specific effect of the polypeptide after the interaction with Swan-71 cells. Finally, the increase in CD4+CD25+FoxP3+ frequency was prevented by an anti–TGF-β Ab and a VIP antagonist. These results suggest that VIP could have an active role in the immunoregulatory processes operating in the maternal–placental interface by contributing to the induction of Tregs through a mechanism involving TGF-β1. © Society for Leukocyte Biology. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v98_n1_p49_Fraccaroli http://hdl.handle.net/20.500.12110/paper_07415400_v98_n1_p49_Fraccaroli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Early pregnancy iTreg TGF-β Tolerance CD4 antigen gamma interferon interleukin 10 interleukin 17 polypeptide transcription factor FOXP3 transforming growth factor beta transforming growth factor beta antibody vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor interleukin 10 transforming growth factor beta1 vasoactive intestinal polypeptide Article cell interaction cell population cell suspension coculture controlled study cytokine production DNA cross linking DNA synthesis female first trimester pregnancy human human cell immune response in vitro study intracellular space leukocyte peripheral blood mononuclear cell priority journal regulatory T lymphocyte trophoblast biosynthesis cell communication cell line cytology drug effects immunology leukocyte metabolism pregnancy real time polymerase chain reaction regulatory T lymphocyte trophoblast Cell Communication Cell Line Coculture Techniques Female Humans In Vitro Techniques Interleukin-10 Leukocytes Pregnancy Pregnancy Trimester, First Real-Time Polymerase Chain Reaction T-Lymphocytes, Regulatory Transforming Growth Factor beta1 Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
Early pregnancy iTreg TGF-β Tolerance CD4 antigen gamma interferon interleukin 10 interleukin 17 polypeptide transcription factor FOXP3 transforming growth factor beta transforming growth factor beta antibody vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor interleukin 10 transforming growth factor beta1 vasoactive intestinal polypeptide Article cell interaction cell population cell suspension coculture controlled study cytokine production DNA cross linking DNA synthesis female first trimester pregnancy human human cell immune response in vitro study intracellular space leukocyte peripheral blood mononuclear cell priority journal regulatory T lymphocyte trophoblast biosynthesis cell communication cell line cytology drug effects immunology leukocyte metabolism pregnancy real time polymerase chain reaction regulatory T lymphocyte trophoblast Cell Communication Cell Line Coculture Techniques Female Humans In Vitro Techniques Interleukin-10 Leukocytes Pregnancy Pregnancy Trimester, First Real-Time Polymerase Chain Reaction T-Lymphocytes, Regulatory Transforming Growth Factor beta1 Trophoblasts Vasoactive Intestinal Peptide VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| topic_facet |
Early pregnancy iTreg TGF-β Tolerance CD4 antigen gamma interferon interleukin 10 interleukin 17 polypeptide transcription factor FOXP3 transforming growth factor beta transforming growth factor beta antibody vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist vasoactive intestinal polypeptide receptor interleukin 10 transforming growth factor beta1 vasoactive intestinal polypeptide Article cell interaction cell population cell suspension coculture controlled study cytokine production DNA cross linking DNA synthesis female first trimester pregnancy human human cell immune response in vitro study intracellular space leukocyte peripheral blood mononuclear cell priority journal regulatory T lymphocyte trophoblast biosynthesis cell communication cell line cytology drug effects immunology leukocyte metabolism pregnancy real time polymerase chain reaction regulatory T lymphocyte trophoblast Cell Communication Cell Line Coculture Techniques Female Humans In Vitro Techniques Interleukin-10 Leukocytes Pregnancy Pregnancy Trimester, First Real-Time Polymerase Chain Reaction T-Lymphocytes, Regulatory Transforming Growth Factor beta1 Trophoblasts Vasoactive Intestinal Peptide |
| description |
Inducible regulatory T cells (Tregs) exert a timely and efficient immunosuppressive action at the critical peri-implantation stage essential for maternal tolerance to the conceptus. Vasoactive intestinal peptide (VIP) promotes anti-inflammatory and tolerogenic profiles through binding to VIP receptors on immune cells. We evaluated whether VIP produced by trophoblast cells induces Tregs during the early interaction of maternal leukocytes with trophoblast cells, thus contributing to maternal tolerance. We used an in vitro model of maternal leukocyte–trophoblast cell interaction represented by cocultures of fertile women’s PBMCs with a human trophoblast cell line (Swan-71) and evaluated the effect of VIP added exogenously and of the endogenous polypeptide. VIP increased the frequency of CD4+CD25+FoxP3+ cells after coculture, and these cells were able to suppress the maternal alloresponse. VIP also increased the frequency of CD4+IL10+ and CD4+TGFβ+ cells, but it did not modulate IFN-γ or IL-17 production. Swan-71 secreted VIP, and their coculture with maternal PBMCs significantly increased the frequency of Tregs. This effect was even more pronounced if the trophoblast cells had been pretreated with VIP. In both situations, the VIP antagonist prevented the increase in the frequency of CD4+Foxp3+ cells, reflecting a specific effect of the polypeptide after the interaction with Swan-71 cells. Finally, the increase in CD4+CD25+FoxP3+ frequency was prevented by an anti–TGF-β Ab and a VIP antagonist. These results suggest that VIP could have an active role in the immunoregulatory processes operating in the maternal–placental interface by contributing to the induction of Tregs through a mechanism involving TGF-β1. © Society for Leukocyte Biology. |
| title |
VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| title_short |
VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| title_full |
VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| title_fullStr |
VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| title_full_unstemmed |
VIP boosts regulatory T cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| title_sort |
vip boosts regulatory t cell induction by trophoblast cells in an in vitro model of trophoblast–maternal leukocyte interaction |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v98_n1_p49_Fraccaroli http://hdl.handle.net/20.500.12110/paper_07415400_v98_n1_p49_Fraccaroli |
| _version_ |
1768545651701841920 |