Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae
The Xdh (rosy) gene is one of the best studied in the Drosophila genus from an evolutionary viewpoint. Here we analyze nucleotide variation in a 1875-bp fragment of the second exon of Xdh in Argentinian populations of the cactophilic D. buzzatii and its sibling D. koepferae. The major electrophoreti...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v21_n1_p141_Piccinali http://hdl.handle.net/20.500.12110/paper_07374038_v21_n1_p141_Piccinali |
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paper:paper_07374038_v21_n1_p141_Piccinali2025-07-30T18:20:50Z Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae Cactophilic Drosophila Negative selection Population expansion Population structure Xdh allele amino acid sequence amino acid substitution article DNA sequence Drosophila electrophoresis female gene gene locus genetic polymorphism genetic variability molecular genetics nonhuman nucleotide sequence phylogeny population genetics population structure sequence analysis species difference Xdh gene Amino Acid Sequence Analysis of Variance Animals Argentina Base Sequence Cactaceae DNA Primers Drosophila Exons Genetics, Population Geography Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Species Specificity Symbiosis Variation (Genetics) Xanthine Dehydrogenase Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda The Xdh (rosy) gene is one of the best studied in the Drosophila genus from an evolutionary viewpoint. Here we analyze nucleotide variation in a 1875-bp fragment of the second exon of Xdh in Argentinian populations of the cactophilic D. buzzatii and its sibling D. koepferae. The major electrophoretic alleles of D. buzzatii not only lack diagnostic amino acids in the region studied but also differ on average from each other by four to 13 amino acid changes. Our data also suggest that D. buzzatii populations belonging to different phytogeographic regions are not genetically differentiated, whereas D. koepferae exhibits a significant pattern of population structure. The Xdh region studied is twice as polymorphic in D. buzzatii as in D. koepferae. Differences in historical population size or in recombinational environment between species could account for the differences in the level of nucleotide variation. In both species, the Xdh region exhibits a great number of singletons, which significantly departs from the frequency spectrum expected under neutrality for nonsynonymous sites and also for synonymous sites in D. buzzatii. These excesses of singletons could be the signature of a recent population expansion in D. buzzatii, whereas they may be simply explained as the result of negative selection in D. koepferae. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v21_n1_p141_Piccinali http://hdl.handle.net/20.500.12110/paper_07374038_v21_n1_p141_Piccinali |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cactophilic Drosophila Negative selection Population expansion Population structure Xdh allele amino acid sequence amino acid substitution article DNA sequence Drosophila electrophoresis female gene gene locus genetic polymorphism genetic variability molecular genetics nonhuman nucleotide sequence phylogeny population genetics population structure sequence analysis species difference Xdh gene Amino Acid Sequence Analysis of Variance Animals Argentina Base Sequence Cactaceae DNA Primers Drosophila Exons Genetics, Population Geography Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Species Specificity Symbiosis Variation (Genetics) Xanthine Dehydrogenase Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda |
| spellingShingle |
Cactophilic Drosophila Negative selection Population expansion Population structure Xdh allele amino acid sequence amino acid substitution article DNA sequence Drosophila electrophoresis female gene gene locus genetic polymorphism genetic variability molecular genetics nonhuman nucleotide sequence phylogeny population genetics population structure sequence analysis species difference Xdh gene Amino Acid Sequence Analysis of Variance Animals Argentina Base Sequence Cactaceae DNA Primers Drosophila Exons Genetics, Population Geography Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Species Specificity Symbiosis Variation (Genetics) Xanthine Dehydrogenase Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae |
| topic_facet |
Cactophilic Drosophila Negative selection Population expansion Population structure Xdh allele amino acid sequence amino acid substitution article DNA sequence Drosophila electrophoresis female gene gene locus genetic polymorphism genetic variability molecular genetics nonhuman nucleotide sequence phylogeny population genetics population structure sequence analysis species difference Xdh gene Amino Acid Sequence Analysis of Variance Animals Argentina Base Sequence Cactaceae DNA Primers Drosophila Exons Genetics, Population Geography Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Species Specificity Symbiosis Variation (Genetics) Xanthine Dehydrogenase Arachnida Drosophila buzzatii Drosophila koepferae Hexapoda |
| description |
The Xdh (rosy) gene is one of the best studied in the Drosophila genus from an evolutionary viewpoint. Here we analyze nucleotide variation in a 1875-bp fragment of the second exon of Xdh in Argentinian populations of the cactophilic D. buzzatii and its sibling D. koepferae. The major electrophoretic alleles of D. buzzatii not only lack diagnostic amino acids in the region studied but also differ on average from each other by four to 13 amino acid changes. Our data also suggest that D. buzzatii populations belonging to different phytogeographic regions are not genetically differentiated, whereas D. koepferae exhibits a significant pattern of population structure. The Xdh region studied is twice as polymorphic in D. buzzatii as in D. koepferae. Differences in historical population size or in recombinational environment between species could account for the differences in the level of nucleotide variation. In both species, the Xdh region exhibits a great number of singletons, which significantly departs from the frequency spectrum expected under neutrality for nonsynonymous sites and also for synonymous sites in D. buzzatii. These excesses of singletons could be the signature of a recent population expansion in D. buzzatii, whereas they may be simply explained as the result of negative selection in D. koepferae. |
| title |
Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae |
| title_short |
Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae |
| title_full |
Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae |
| title_fullStr |
Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae |
| title_full_unstemmed |
Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae |
| title_sort |
comparative molecular population genetics of the xdh locus in the cactophilic sibling species drosophila buzzatii and d. koepferae |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v21_n1_p141_Piccinali http://hdl.handle.net/20.500.12110/paper_07374038_v21_n1_p141_Piccinali |
| _version_ |
1840327331853369344 |