Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats
The Asr2 gene encodes a putative transcription factor that is up-regulated in leaves and roots of tomato plants exposed to water-deficit stress. This gene was first cloned and characterized in a cultivar of commercial tomato (Lycopersicon esculentum cv, Ailsa Craig). In this work, we report the comp...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v20_n12_p1955_Frankel http://hdl.handle.net/20.500.12110/paper_07374038_v20_n12_p1955_Frankel |
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paper:paper_07374038_v20_n12_p1955_Frankel2023-06-08T15:44:19Z Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats Adaptive evolution Asr genes Lycopersicon Tomato Water stress ASR2 protein gene product transcription factor unclassified drug adaptive evolution amino acid substitution article Asr2 gene desert evolutionary adaptation gene expression Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum cersiforme Lycopersicon hirsutum Lycopersicon peruvianum glandulosum Lycopersicon peruvianum humifusum molecular cloning nonhuman nucleic acid base substitution nucleotide sequence phylogenetic tree plant genetics protein conformation statistical model tomato water deficit water stress Amino Acid Sequence Amino Acid Substitution Base Sequence Environment Evolution Likelihood Functions Lycopersicon esculentum Molecular Sequence Data Phylogeny Plant Proteins Species Specificity Darwinia Embryophyta Lycopersicon Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum Lycopersicon hirsutum Lycopersicon peruvianum var. humifusum The Asr2 gene encodes a putative transcription factor that is up-regulated in leaves and roots of tomato plants exposed to water-deficit stress. This gene was first cloned and characterized in a cultivar of commercial tomato (Lycopersicon esculentum cv, Ailsa Craig). In this work, we report the complete coding sequences of the orthologous Asr2 genes in six wild tomato lineages: L. hirsutum, L. cheesmanii, L. escutentum v. cerasiforme, L. chilense, L. peruviamml v. humifusum and L. peruvianum f. glandulosum. Estimates of the Ka/Ks ratio (ω) in pairwise comparisons within the genus Lycopersicon were equal or greater than 1 (a signature of adaptive evolution) when involving L. chilense and L. peruvianum v, humifusum. Interestingly, these two species are distinct from the others in their adaptation to dry habitats. We also mapped the detected substitutions onto a phylogenetic tree of the genus Lycopersicon. Remarkably, there are two and three amino acid substitutions, which contrast with the absence of synonymous substitutions along the terminal branches leading to L. chilense and L. peruvianum v. humifusum, respectively. Likelihood ratio tests confirmed that ω values in the branches leading to these species are significantly different from the remaining branches of the tree. Moreover, inferred changes in the branches leading to these species that inhabit dry areas are nonconservative and may be associated with dramatic alterations in ASR2 protein conformation, In this work, we demonstrate accelerated rates of amino acid substitutions in the Asr2 gene of tomato lineages living in dry habitats, thus giving support to the hypothesis of adaptive Darwinian evolution. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v20_n12_p1955_Frankel http://hdl.handle.net/20.500.12110/paper_07374038_v20_n12_p1955_Frankel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adaptive evolution Asr genes Lycopersicon Tomato Water stress ASR2 protein gene product transcription factor unclassified drug adaptive evolution amino acid substitution article Asr2 gene desert evolutionary adaptation gene expression Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum cersiforme Lycopersicon hirsutum Lycopersicon peruvianum glandulosum Lycopersicon peruvianum humifusum molecular cloning nonhuman nucleic acid base substitution nucleotide sequence phylogenetic tree plant genetics protein conformation statistical model tomato water deficit water stress Amino Acid Sequence Amino Acid Substitution Base Sequence Environment Evolution Likelihood Functions Lycopersicon esculentum Molecular Sequence Data Phylogeny Plant Proteins Species Specificity Darwinia Embryophyta Lycopersicon Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum Lycopersicon hirsutum Lycopersicon peruvianum var. humifusum |
| spellingShingle |
Adaptive evolution Asr genes Lycopersicon Tomato Water stress ASR2 protein gene product transcription factor unclassified drug adaptive evolution amino acid substitution article Asr2 gene desert evolutionary adaptation gene expression Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum cersiforme Lycopersicon hirsutum Lycopersicon peruvianum glandulosum Lycopersicon peruvianum humifusum molecular cloning nonhuman nucleic acid base substitution nucleotide sequence phylogenetic tree plant genetics protein conformation statistical model tomato water deficit water stress Amino Acid Sequence Amino Acid Substitution Base Sequence Environment Evolution Likelihood Functions Lycopersicon esculentum Molecular Sequence Data Phylogeny Plant Proteins Species Specificity Darwinia Embryophyta Lycopersicon Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum Lycopersicon hirsutum Lycopersicon peruvianum var. humifusum Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats |
| topic_facet |
Adaptive evolution Asr genes Lycopersicon Tomato Water stress ASR2 protein gene product transcription factor unclassified drug adaptive evolution amino acid substitution article Asr2 gene desert evolutionary adaptation gene expression Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum cersiforme Lycopersicon hirsutum Lycopersicon peruvianum glandulosum Lycopersicon peruvianum humifusum molecular cloning nonhuman nucleic acid base substitution nucleotide sequence phylogenetic tree plant genetics protein conformation statistical model tomato water deficit water stress Amino Acid Sequence Amino Acid Substitution Base Sequence Environment Evolution Likelihood Functions Lycopersicon esculentum Molecular Sequence Data Phylogeny Plant Proteins Species Specificity Darwinia Embryophyta Lycopersicon Lycopersicon cheesmanii Lycopersicon chilense Lycopersicon esculentum Lycopersicon hirsutum Lycopersicon peruvianum var. humifusum |
| description |
The Asr2 gene encodes a putative transcription factor that is up-regulated in leaves and roots of tomato plants exposed to water-deficit stress. This gene was first cloned and characterized in a cultivar of commercial tomato (Lycopersicon esculentum cv, Ailsa Craig). In this work, we report the complete coding sequences of the orthologous Asr2 genes in six wild tomato lineages: L. hirsutum, L. cheesmanii, L. escutentum v. cerasiforme, L. chilense, L. peruviamml v. humifusum and L. peruvianum f. glandulosum. Estimates of the Ka/Ks ratio (ω) in pairwise comparisons within the genus Lycopersicon were equal or greater than 1 (a signature of adaptive evolution) when involving L. chilense and L. peruvianum v, humifusum. Interestingly, these two species are distinct from the others in their adaptation to dry habitats. We also mapped the detected substitutions onto a phylogenetic tree of the genus Lycopersicon. Remarkably, there are two and three amino acid substitutions, which contrast with the absence of synonymous substitutions along the terminal branches leading to L. chilense and L. peruvianum v. humifusum, respectively. Likelihood ratio tests confirmed that ω values in the branches leading to these species are significantly different from the remaining branches of the tree. Moreover, inferred changes in the branches leading to these species that inhabit dry areas are nonconservative and may be associated with dramatic alterations in ASR2 protein conformation, In this work, we demonstrate accelerated rates of amino acid substitutions in the Asr2 gene of tomato lineages living in dry habitats, thus giving support to the hypothesis of adaptive Darwinian evolution. |
| title |
Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats |
| title_short |
Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats |
| title_full |
Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats |
| title_fullStr |
Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats |
| title_full_unstemmed |
Adaptive Evolution of the Water Stress-Induced Gene Asr2 in Lycopersicon Species Dwelling in Arid Habitats |
| title_sort |
adaptive evolution of the water stress-induced gene asr2 in lycopersicon species dwelling in arid habitats |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07374038_v20_n12_p1955_Frankel http://hdl.handle.net/20.500.12110/paper_07374038_v20_n12_p1955_Frankel |
| _version_ |
1768542040710184960 |