αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarc...
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1990
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07357907_v8_n3-4_p365_Elizalde http://hdl.handle.net/20.500.12110/paper_07357907_v8_n3-4_p365_Elizalde |
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paper:paper_07357907_v8_n3-4_p365_Elizalde2023-06-08T15:44:09Z αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms transforming growth factor article cancer cell carcinoma cell culture cell growth dna synthesis human human cell priority journal sarcoma Carcinoma, Squamous Cell Cell Transformation, Neoplastic Cells, Cultured Chromatography, Gel DNA Dose-Response Relationship, Drug Fibroblasts Human In Vitro Lung Neoplasms Receptor, Epidermal Growth Factor Rhabdomyosarcoma Support, Non-U.S. Gov't Thoracic Neoplasms Transforming Growth Factor alpha Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarcoma. In both tumors, low-molecular-weight transforming activities (4000-6000) that were not potentiated by epidermal growth factor (EGF), competed for binding to the EGF receptor, possessed mitogenic activity on NRK fibroblasts arrested in serum-deprived medium, and did not show inhibitory effects on DNA synthesis induced by EGF and insulin in NRK cells. Other TGFs with molecular weights 9000 to 48,000, were also found in the malignant tissues examined; these TGFs, were not potentiated by EGF, did not compete for binding to the EGF receptor, were not mitogenic for NRK cells, and acted as potent inhibitors of DNA synthesis induced by EGF and insulin in NRK cells. These results demonstrate that growth-promoting activities, and modulating agents that can act as either enhancers or inhibitors of cell proliferation, are present in neoplastic tissues of different embryologic origin and histologic type. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07357907_v8_n3-4_p365_Elizalde http://hdl.handle.net/20.500.12110/paper_07357907_v8_n3-4_p365_Elizalde |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
transforming growth factor article cancer cell carcinoma cell culture cell growth dna synthesis human human cell priority journal sarcoma Carcinoma, Squamous Cell Cell Transformation, Neoplastic Cells, Cultured Chromatography, Gel DNA Dose-Response Relationship, Drug Fibroblasts Human In Vitro Lung Neoplasms Receptor, Epidermal Growth Factor Rhabdomyosarcoma Support, Non-U.S. Gov't Thoracic Neoplasms Transforming Growth Factor alpha |
| spellingShingle |
transforming growth factor article cancer cell carcinoma cell culture cell growth dna synthesis human human cell priority journal sarcoma Carcinoma, Squamous Cell Cell Transformation, Neoplastic Cells, Cultured Chromatography, Gel DNA Dose-Response Relationship, Drug Fibroblasts Human In Vitro Lung Neoplasms Receptor, Epidermal Growth Factor Rhabdomyosarcoma Support, Non-U.S. Gov't Thoracic Neoplasms Transforming Growth Factor alpha αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| topic_facet |
transforming growth factor article cancer cell carcinoma cell culture cell growth dna synthesis human human cell priority journal sarcoma Carcinoma, Squamous Cell Cell Transformation, Neoplastic Cells, Cultured Chromatography, Gel DNA Dose-Response Relationship, Drug Fibroblasts Human In Vitro Lung Neoplasms Receptor, Epidermal Growth Factor Rhabdomyosarcoma Support, Non-U.S. Gov't Thoracic Neoplasms Transforming Growth Factor alpha |
| description |
Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarcoma. In both tumors, low-molecular-weight transforming activities (4000-6000) that were not potentiated by epidermal growth factor (EGF), competed for binding to the EGF receptor, possessed mitogenic activity on NRK fibroblasts arrested in serum-deprived medium, and did not show inhibitory effects on DNA synthesis induced by EGF and insulin in NRK cells. Other TGFs with molecular weights 9000 to 48,000, were also found in the malignant tissues examined; these TGFs, were not potentiated by EGF, did not compete for binding to the EGF receptor, were not mitogenic for NRK cells, and acted as potent inhibitors of DNA synthesis induced by EGF and insulin in NRK cells. These results demonstrate that growth-promoting activities, and modulating agents that can act as either enhancers or inhibitors of cell proliferation, are present in neoplastic tissues of different embryologic origin and histologic type. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| title |
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| title_short |
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| title_full |
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| title_fullStr |
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| title_full_unstemmed |
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| title_sort |
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07357907_v8_n3-4_p365_Elizalde http://hdl.handle.net/20.500.12110/paper_07357907_v8_n3-4_p365_Elizalde |
| _version_ |
1768544415347900416 |