Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity
Background: The use of microorganisms for the synthesis of nanoparticles (NPs) is relatively new in basic research and technology areas. Purpose: This work was conducted to optimized the biosynthesis of iron NPs intra- and extracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluate t...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07248741_v34_n3_p591_Crespo http://hdl.handle.net/20.500.12110/paper_07248741_v34_n3_p591_Crespo |
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paper:paper_07248741_v34_n3_p591_Crespo2023-06-08T15:43:42Z Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity anticoagulant activity Escherichia coli iron nanoparticles microbial biosynthesis Pseudomonas aeruginosa Background: The use of microorganisms for the synthesis of nanoparticles (NPs) is relatively new in basic research and technology areas. Purpose: This work was conducted to optimized the biosynthesis of iron NPs intra- and extracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluate their anticoagulant activity. Study Design/Methods: The structures and properties of the iron NPs were investigated by Ultraviolet–visible (UV-vis) spectroscopy, Zeta potential, Dynamic light scattering (DLS), Field emission scanning electron microscope (FESEM)/ Energy dispersive X-ray (EDX) and transmission electron microscopy (TEM). Anticoagulant activity was determined by conducting trials of Thrombin Time (TT), Activated Partial Prothrombin Time (APTT) and Prothrombin Time (PT). Results: UV-vis spectrum of the aqueous medium containing iron NPs showed a peak at 275 nm. The forming of iron NPs was confirmed by FESEM/ EDX, and TEM. The morphology was spherical shapes mostly with low polydispersity and the average particle diameter was 23 ± 1 nm. Iron NPs showed anticoagulant activity by the activation of extrinsic pathway. Conclusion: The eco-friendly process of biosynthesis of iron NPs employing prokaryotic microorganisms presents a good anticoagulant activity. This could be explored as promising candidates for a variety of biomedical and pharmaceutical applications. © 2016, Springer Science+Business Media New York. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07248741_v34_n3_p591_Crespo http://hdl.handle.net/20.500.12110/paper_07248741_v34_n3_p591_Crespo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
anticoagulant activity Escherichia coli iron nanoparticles microbial biosynthesis Pseudomonas aeruginosa |
| spellingShingle |
anticoagulant activity Escherichia coli iron nanoparticles microbial biosynthesis Pseudomonas aeruginosa Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity |
| topic_facet |
anticoagulant activity Escherichia coli iron nanoparticles microbial biosynthesis Pseudomonas aeruginosa |
| description |
Background: The use of microorganisms for the synthesis of nanoparticles (NPs) is relatively new in basic research and technology areas. Purpose: This work was conducted to optimized the biosynthesis of iron NPs intra- and extracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluate their anticoagulant activity. Study Design/Methods: The structures and properties of the iron NPs were investigated by Ultraviolet–visible (UV-vis) spectroscopy, Zeta potential, Dynamic light scattering (DLS), Field emission scanning electron microscope (FESEM)/ Energy dispersive X-ray (EDX) and transmission electron microscopy (TEM). Anticoagulant activity was determined by conducting trials of Thrombin Time (TT), Activated Partial Prothrombin Time (APTT) and Prothrombin Time (PT). Results: UV-vis spectrum of the aqueous medium containing iron NPs showed a peak at 275 nm. The forming of iron NPs was confirmed by FESEM/ EDX, and TEM. The morphology was spherical shapes mostly with low polydispersity and the average particle diameter was 23 ± 1 nm. Iron NPs showed anticoagulant activity by the activation of extrinsic pathway. Conclusion: The eco-friendly process of biosynthesis of iron NPs employing prokaryotic microorganisms presents a good anticoagulant activity. This could be explored as promising candidates for a variety of biomedical and pharmaceutical applications. © 2016, Springer Science+Business Media New York. |
| title |
Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity |
| title_short |
Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity |
| title_full |
Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity |
| title_fullStr |
Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity |
| title_full_unstemmed |
Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity |
| title_sort |
intra- and extracellular biosynthesis and characterization of iron nanoparticles from prokaryotic microorganisms with anticoagulant activity |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07248741_v34_n3_p591_Crespo http://hdl.handle.net/20.500.12110/paper_07248741_v34_n3_p591_Crespo |
| _version_ |
1768543040575635456 |