Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta

The chronic stress induces functional adaptations in the hypothalamo-pituitary- adrenocortical (HPA) and in the sympathetic-medullary- adrenal axis (SAM). Both axis are considered vital regulators of the homeostasis in vertebrates (Seyle, 1936; Ostrandrer et al., 2006. On the other hand, the placent...

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Detalles Bibliográficos
Publicado: 2007
Materias:
Chronic stress
Placental lactogen
Prolactin
Rat
SAM
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07179367_v25_n1_p85_Romanini
http://hdl.handle.net/20.500.12110/paper_07179367_v25_n1_p85_Romanini
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_07179367_v25_n1_p85_Romanini
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spelling paper:paper_07179367_v25_n1_p85_Romanini2023-06-08T15:43:05Z Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta Chronic stress Placental lactogen Prolactin Rat SAM The chronic stress induces functional adaptations in the hypothalamo-pituitary- adrenocortical (HPA) and in the sympathetic-medullary- adrenal axis (SAM). Both axis are considered vital regulators of the homeostasis in vertebrates (Seyle, 1936; Ostrandrer et al., 2006. On the other hand, the placenta provides highly specialized functions during gestation that are critical for the normal development of the embryo/fetus (Soares et al., 1991). We hypothesized that the chronic immobilization (IMO) stress in pregnancy rats produces alterations in prolactin concentrations in placental tissue and also changes in the response of SAM axis. Chronic stress by IMO was applied on days 12, 17 and 21 of pregnancy rats. Relative concentrations and localization of placental lactogen-II (PL-II) and the PRL- like protein A (PLP-A) in chorioalantoic placenta were estimated by Immunoblotting and Immunocytochemical analysis. The levels of catecholamines metabolite, acid 3-metoxi 4-hidroximandélico (VMA), were analyzed in stressed rats urines on 6,12,17,21 days of pregnancy, by HPLC, in order to determine the response of SAM axis. During the days of the pregnancy studied, chronic stress did not induce any changes neither in the localization nor in placental concentrations of PL-II and PLP-A. The VMA values in stressed mothers urines increased on the day 6 respecting the control ones at the same time of pregnancy. VMA values in stressed rats at 21 days of pregnancy are smaller than the respective controls. We conclude that the chronic stressed mothers activated the SAM axis at the beginning of pregnancy and then they diminished the metabolites catecholamines that were interpreted as a stress adaptation coincident with normal concentrations of both placentary prolactines at this stage of the pregnancy. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07179367_v25_n1_p85_Romanini http://hdl.handle.net/20.500.12110/paper_07179367_v25_n1_p85_Romanini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chronic stress
Placental lactogen
Prolactin
Rat
SAM
spellingShingle Chronic stress
Placental lactogen
Prolactin
Rat
SAM
Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta
topic_facet Chronic stress
Placental lactogen
Prolactin
Rat
SAM
description The chronic stress induces functional adaptations in the hypothalamo-pituitary- adrenocortical (HPA) and in the sympathetic-medullary- adrenal axis (SAM). Both axis are considered vital regulators of the homeostasis in vertebrates (Seyle, 1936; Ostrandrer et al., 2006. On the other hand, the placenta provides highly specialized functions during gestation that are critical for the normal development of the embryo/fetus (Soares et al., 1991). We hypothesized that the chronic immobilization (IMO) stress in pregnancy rats produces alterations in prolactin concentrations in placental tissue and also changes in the response of SAM axis. Chronic stress by IMO was applied on days 12, 17 and 21 of pregnancy rats. Relative concentrations and localization of placental lactogen-II (PL-II) and the PRL- like protein A (PLP-A) in chorioalantoic placenta were estimated by Immunoblotting and Immunocytochemical analysis. The levels of catecholamines metabolite, acid 3-metoxi 4-hidroximandélico (VMA), were analyzed in stressed rats urines on 6,12,17,21 days of pregnancy, by HPLC, in order to determine the response of SAM axis. During the days of the pregnancy studied, chronic stress did not induce any changes neither in the localization nor in placental concentrations of PL-II and PLP-A. The VMA values in stressed mothers urines increased on the day 6 respecting the control ones at the same time of pregnancy. VMA values in stressed rats at 21 days of pregnancy are smaller than the respective controls. We conclude that the chronic stressed mothers activated the SAM axis at the beginning of pregnancy and then they diminished the metabolites catecholamines that were interpreted as a stress adaptation coincident with normal concentrations of both placentary prolactines at this stage of the pregnancy.
title Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta
title_short Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta
title_full Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta
title_fullStr Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta
title_full_unstemmed Relative concentrations of placental lactogen II and PRL-like protein-A in stressed rats placenta
title_sort relative concentrations of placental lactogen ii and prl-like protein-a in stressed rats placenta
publishDate 2007
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07179367_v25_n1_p85_Romanini
http://hdl.handle.net/20.500.12110/paper_07179367_v25_n1_p85_Romanini
_version_ 1768543613977886720

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