Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line
We evaluated the effect of interferon-alpha2b as a chemosensitiser on HCT-15 cell line in treatment with doxorubicin. Chemosensitivity was determined by [3H]-thymidine incorporation and tetrazolium assays. The levels of expression of P-glycoprotein, Bcl-2 oncoprotein and HLA-ABC complex, and cell cy...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti http://hdl.handle.net/20.500.12110/paper_03929078_v20_n3_p393_Gritti |
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paper:paper_03929078_v20_n3_p393_Gritti2023-06-08T15:41:01Z Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line Apoptosis Bcl-2 HLA-ABC Interferon-alpha 2b P-170 alpha2b interferon doxorubicin glycoprotein P HLA antigen protein bcl 2 tetrazolium thymidine tritium apoptosis article cancer cell culture cell cycle G2 phase cell viability concentration response controlled study drug potentiation flow cytometry gene expression human human cell multidrug resistance priority journal Adenocarcinoma Antineoplastic Agents Apoptosis Cell Cycle Cell Division Cell Survival Colonic Neoplasms Doxorubicin Drug Resistance, Multiple Drug Synergism G2 Phase Histocompatibility Antigens Class I HLA Antigens HLA-A Antigens HLA-C Antigens Humans Interferon Alfa-2b Membrane Proteins Mitosis Oncogene Protein v-cbl P-Glycoprotein Retroviridae Proteins, Oncogenic Tumor Cells, Cultured We evaluated the effect of interferon-alpha2b as a chemosensitiser on HCT-15 cell line in treatment with doxorubicin. Chemosensitivity was determined by [3H]-thymidine incorporation and tetrazolium assays. The levels of expression of P-glycoprotein, Bcl-2 oncoprotein and HLA-ABC complex, and cell cycle/apoptosis analysis were determined by flow cytometry. Dox 50 ng/ml - IFN α 2b 500 IU/ml treatment inhibited cell proliferation (47.2 ± 1.4%, p< 0.0001; MTT assay: 40.6 ± 1.2%, p < 0.0001) and augmented the expression of P-170, Bcl-2 and HLA-ABC, while it didn't exert apoptosis, producing a slight G2/M arrest. A concentration of IFN-α2b, that by itself is not cytotoxic, can potentiate the efficacy of the anticancer drug. This effect is not due to a down-modulation of P-170. The absence of apoptosis and augmented levels of Bcl-2 expression suggests that this could be one of the mechanisms of drug resistance exerted by these cells. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti http://hdl.handle.net/20.500.12110/paper_03929078_v20_n3_p393_Gritti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Bcl-2 HLA-ABC Interferon-alpha 2b P-170 alpha2b interferon doxorubicin glycoprotein P HLA antigen protein bcl 2 tetrazolium thymidine tritium apoptosis article cancer cell culture cell cycle G2 phase cell viability concentration response controlled study drug potentiation flow cytometry gene expression human human cell multidrug resistance priority journal Adenocarcinoma Antineoplastic Agents Apoptosis Cell Cycle Cell Division Cell Survival Colonic Neoplasms Doxorubicin Drug Resistance, Multiple Drug Synergism G2 Phase Histocompatibility Antigens Class I HLA Antigens HLA-A Antigens HLA-C Antigens Humans Interferon Alfa-2b Membrane Proteins Mitosis Oncogene Protein v-cbl P-Glycoprotein Retroviridae Proteins, Oncogenic Tumor Cells, Cultured |
| spellingShingle |
Apoptosis Bcl-2 HLA-ABC Interferon-alpha 2b P-170 alpha2b interferon doxorubicin glycoprotein P HLA antigen protein bcl 2 tetrazolium thymidine tritium apoptosis article cancer cell culture cell cycle G2 phase cell viability concentration response controlled study drug potentiation flow cytometry gene expression human human cell multidrug resistance priority journal Adenocarcinoma Antineoplastic Agents Apoptosis Cell Cycle Cell Division Cell Survival Colonic Neoplasms Doxorubicin Drug Resistance, Multiple Drug Synergism G2 Phase Histocompatibility Antigens Class I HLA Antigens HLA-A Antigens HLA-C Antigens Humans Interferon Alfa-2b Membrane Proteins Mitosis Oncogene Protein v-cbl P-Glycoprotein Retroviridae Proteins, Oncogenic Tumor Cells, Cultured Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| topic_facet |
Apoptosis Bcl-2 HLA-ABC Interferon-alpha 2b P-170 alpha2b interferon doxorubicin glycoprotein P HLA antigen protein bcl 2 tetrazolium thymidine tritium apoptosis article cancer cell culture cell cycle G2 phase cell viability concentration response controlled study drug potentiation flow cytometry gene expression human human cell multidrug resistance priority journal Adenocarcinoma Antineoplastic Agents Apoptosis Cell Cycle Cell Division Cell Survival Colonic Neoplasms Doxorubicin Drug Resistance, Multiple Drug Synergism G2 Phase Histocompatibility Antigens Class I HLA Antigens HLA-A Antigens HLA-C Antigens Humans Interferon Alfa-2b Membrane Proteins Mitosis Oncogene Protein v-cbl P-Glycoprotein Retroviridae Proteins, Oncogenic Tumor Cells, Cultured |
| description |
We evaluated the effect of interferon-alpha2b as a chemosensitiser on HCT-15 cell line in treatment with doxorubicin. Chemosensitivity was determined by [3H]-thymidine incorporation and tetrazolium assays. The levels of expression of P-glycoprotein, Bcl-2 oncoprotein and HLA-ABC complex, and cell cycle/apoptosis analysis were determined by flow cytometry. Dox 50 ng/ml - IFN α 2b 500 IU/ml treatment inhibited cell proliferation (47.2 ± 1.4%, p< 0.0001; MTT assay: 40.6 ± 1.2%, p < 0.0001) and augmented the expression of P-170, Bcl-2 and HLA-ABC, while it didn't exert apoptosis, producing a slight G2/M arrest. A concentration of IFN-α2b, that by itself is not cytotoxic, can potentiate the efficacy of the anticancer drug. This effect is not due to a down-modulation of P-170. The absence of apoptosis and augmented levels of Bcl-2 expression suggests that this could be one of the mechanisms of drug resistance exerted by these cells. |
| title |
Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| title_short |
Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| title_full |
Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| title_fullStr |
Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| title_full_unstemmed |
Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| title_sort |
interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n3_p393_Gritti http://hdl.handle.net/20.500.12110/paper_03929078_v20_n3_p393_Gritti |
| _version_ |
1768541992110784512 |