Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients
Tumour necrosis factor-alpha (TNF-α) is a monocyte (MO)-derived cytokine that plays an essential role in the immunological system. In the present study our aim was to evaluate the levels of TNF-α secreted by MO from cancer patients. Blood MO were obtained from 10 lung cancer patients (LCP), 10 color...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo http://hdl.handle.net/20.500.12110/paper_03929078_v20_n1_p71_Trejo |
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| Sumario: | Tumour necrosis factor-alpha (TNF-α) is a monocyte (MO)-derived cytokine that plays an essential role in the immunological system. In the present study our aim was to evaluate the levels of TNF-α secreted by MO from cancer patients. Blood MO were obtained from 10 lung cancer patients (LCP), 10 colorectal cancer patients (CCP) and 10 healthy donors (HD). TNF-α levels in MO culture supernatants spontaneously (sp) secreted or after stimulation with LPS treatment were evaluated using a commercial ELISA kit (sensibility: 10-1000 pg/ml). Mean values, expressed as pg/ml were: LCP: sp= 452.6 ± 107.2, LPS= 589.5 ± 126.7); CCP: sp= 84.1 ± 25.0, LPS= 437.3 ± 93.2; HD: sp= 74.2 ± 21.5, LPS= 573.5 ± 87.1. We concluded that MO from LCP secrete high levels of TNF-α spontaneously (p< 0.003 versus HD) and it was also observed an absence of response to LPS treatment in the 33% of the cases in these patients. |
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