Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients
Tumour necrosis factor-alpha (TNF-α) is a monocyte (MO)-derived cytokine that plays an essential role in the immunological system. In the present study our aim was to evaluate the levels of TNF-α secreted by MO from cancer patients. Blood MO were obtained from 10 lung cancer patients (LCP), 10 color...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo http://hdl.handle.net/20.500.12110/paper_03929078_v20_n1_p71_Trejo |
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paper:paper_03929078_v20_n1_p71_Trejo2023-06-08T15:41:00Z Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients Colon cancer Lung cancer Monocytes Tumor necrosis factor alfa lipopolysaccharide tumor necrosis factor alpha article clinical article colorectal cancer controlled study cytokine production cytokine release enzyme linked immunosorbent assay human human cell lung cancer monocyte mononuclear cell priority journal tumor immunology Adult Aged Aged, 80 and over Cells, Cultured Colorectal Neoplasms Enzyme-Linked Immunosorbent Assay Humans Lung Neoplasms Middle Aged Monocytes Reference Values Tumor Necrosis Factor-alpha Tumour necrosis factor-alpha (TNF-α) is a monocyte (MO)-derived cytokine that plays an essential role in the immunological system. In the present study our aim was to evaluate the levels of TNF-α secreted by MO from cancer patients. Blood MO were obtained from 10 lung cancer patients (LCP), 10 colorectal cancer patients (CCP) and 10 healthy donors (HD). TNF-α levels in MO culture supernatants spontaneously (sp) secreted or after stimulation with LPS treatment were evaluated using a commercial ELISA kit (sensibility: 10-1000 pg/ml). Mean values, expressed as pg/ml were: LCP: sp= 452.6 ± 107.2, LPS= 589.5 ± 126.7); CCP: sp= 84.1 ± 25.0, LPS= 437.3 ± 93.2; HD: sp= 74.2 ± 21.5, LPS= 573.5 ± 87.1. We concluded that MO from LCP secrete high levels of TNF-α spontaneously (p< 0.003 versus HD) and it was also observed an absence of response to LPS treatment in the 33% of the cases in these patients. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo http://hdl.handle.net/20.500.12110/paper_03929078_v20_n1_p71_Trejo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Colon cancer Lung cancer Monocytes Tumor necrosis factor alfa lipopolysaccharide tumor necrosis factor alpha article clinical article colorectal cancer controlled study cytokine production cytokine release enzyme linked immunosorbent assay human human cell lung cancer monocyte mononuclear cell priority journal tumor immunology Adult Aged Aged, 80 and over Cells, Cultured Colorectal Neoplasms Enzyme-Linked Immunosorbent Assay Humans Lung Neoplasms Middle Aged Monocytes Reference Values Tumor Necrosis Factor-alpha |
| spellingShingle |
Colon cancer Lung cancer Monocytes Tumor necrosis factor alfa lipopolysaccharide tumor necrosis factor alpha article clinical article colorectal cancer controlled study cytokine production cytokine release enzyme linked immunosorbent assay human human cell lung cancer monocyte mononuclear cell priority journal tumor immunology Adult Aged Aged, 80 and over Cells, Cultured Colorectal Neoplasms Enzyme-Linked Immunosorbent Assay Humans Lung Neoplasms Middle Aged Monocytes Reference Values Tumor Necrosis Factor-alpha Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| topic_facet |
Colon cancer Lung cancer Monocytes Tumor necrosis factor alfa lipopolysaccharide tumor necrosis factor alpha article clinical article colorectal cancer controlled study cytokine production cytokine release enzyme linked immunosorbent assay human human cell lung cancer monocyte mononuclear cell priority journal tumor immunology Adult Aged Aged, 80 and over Cells, Cultured Colorectal Neoplasms Enzyme-Linked Immunosorbent Assay Humans Lung Neoplasms Middle Aged Monocytes Reference Values Tumor Necrosis Factor-alpha |
| description |
Tumour necrosis factor-alpha (TNF-α) is a monocyte (MO)-derived cytokine that plays an essential role in the immunological system. In the present study our aim was to evaluate the levels of TNF-α secreted by MO from cancer patients. Blood MO were obtained from 10 lung cancer patients (LCP), 10 colorectal cancer patients (CCP) and 10 healthy donors (HD). TNF-α levels in MO culture supernatants spontaneously (sp) secreted or after stimulation with LPS treatment were evaluated using a commercial ELISA kit (sensibility: 10-1000 pg/ml). Mean values, expressed as pg/ml were: LCP: sp= 452.6 ± 107.2, LPS= 589.5 ± 126.7); CCP: sp= 84.1 ± 25.0, LPS= 437.3 ± 93.2; HD: sp= 74.2 ± 21.5, LPS= 573.5 ± 87.1. We concluded that MO from LCP secrete high levels of TNF-α spontaneously (p< 0.003 versus HD) and it was also observed an absence of response to LPS treatment in the 33% of the cases in these patients. |
| title |
Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| title_short |
Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| title_full |
Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| title_fullStr |
Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| title_full_unstemmed |
Tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| title_sort |
tumor necrosis factor-alfa production by monocytes from lung and colorectal cancer patients |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03929078_v20_n1_p71_Trejo http://hdl.handle.net/20.500.12110/paper_03929078_v20_n1_p71_Trejo |
| _version_ |
1768544915879362560 |