Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
The effect of the in vivo administration of hexachlorobenzene (HCB) (100 mg/100 g bw) for 30 days, on the activities of brown adipose tissue (BAT) lipogenic enzymes, i.e. malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PD) and the mitochondrial non lipogenic enzyme, L-glycerol-3-phosphat...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03914097_v22_n6_p436_Alvarez http://hdl.handle.net/20.500.12110/paper_03914097_v22_n6_p436_Alvarez |
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paper:paper_03914097_v22_n6_p436_Alvarez2023-06-08T15:40:55Z Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time The effect of the in vivo administration of hexachlorobenzene (HCB) (100 mg/100 g bw) for 30 days, on the activities of brown adipose tissue (BAT) lipogenic enzymes, i.e. malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PD) and the mitochondrial non lipogenic enzyme, L-glycerol-3-phosphate dehydrogenase (LG3PD), was studied in male Wistar rats, submitted to various neurohormonal manipulations. BAT ME, G6PD and LG3PD activities showed significant reductions in response to HCB treatment both in euthyroid and surgically thyroidectomized rats, showing that the effect does not depend on the presence of thyroid hormones. These results differ from those obtained for hepatic ME and G6PD activities, which increased in HCB intoxicated rats without alteration in LG3PD. HCB decreased BAT ME activity under BAT denervation. Administration of HCB resulted in time and dose-dependent decreases in the activity of BAT malic enzyme. The basal activity of ME was increased in hypothyroid rats, while that of LG3PD was reduced. A stimulatory effect of receptor-saturating doses of triiodothyronine (T 3 ) (50 μg/100 g body weight) was observed on BAT ME and LG3PD activities in thyroidectomized rats, showing that the enzymes responded to thyroid hormone stimulation in a normal manner. The stimulatory effect of saturating doses of T 3 on ME and LG3PD was reduced by HCB. The results presented herein unequivocally show that brown adipose tissue is a specific target in HCB-induced toxicity, which in turn involves severe alterations in the regulation of BAT lipogenesis. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03914097_v22_n6_p436_Alvarez http://hdl.handle.net/20.500.12110/paper_03914097_v22_n6_p436_Alvarez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time |
| spellingShingle |
Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| topic_facet |
Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time |
| description |
The effect of the in vivo administration of hexachlorobenzene (HCB) (100 mg/100 g bw) for 30 days, on the activities of brown adipose tissue (BAT) lipogenic enzymes, i.e. malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PD) and the mitochondrial non lipogenic enzyme, L-glycerol-3-phosphate dehydrogenase (LG3PD), was studied in male Wistar rats, submitted to various neurohormonal manipulations. BAT ME, G6PD and LG3PD activities showed significant reductions in response to HCB treatment both in euthyroid and surgically thyroidectomized rats, showing that the effect does not depend on the presence of thyroid hormones. These results differ from those obtained for hepatic ME and G6PD activities, which increased in HCB intoxicated rats without alteration in LG3PD. HCB decreased BAT ME activity under BAT denervation. Administration of HCB resulted in time and dose-dependent decreases in the activity of BAT malic enzyme. The basal activity of ME was increased in hypothyroid rats, while that of LG3PD was reduced. A stimulatory effect of receptor-saturating doses of triiodothyronine (T 3 ) (50 μg/100 g body weight) was observed on BAT ME and LG3PD activities in thyroidectomized rats, showing that the enzymes responded to thyroid hormone stimulation in a normal manner. The stimulatory effect of saturating doses of T 3 on ME and LG3PD was reduced by HCB. The results presented herein unequivocally show that brown adipose tissue is a specific target in HCB-induced toxicity, which in turn involves severe alterations in the regulation of BAT lipogenesis. |
| title |
Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| title_short |
Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| title_full |
Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| title_fullStr |
Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| title_full_unstemmed |
Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| title_sort |
effect of hexachlorobenzene on nadph-generating lipogenic enzymes and l-glycerol-3-phosphate dehydrogenase in brown adipose tissue |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03914097_v22_n6_p436_Alvarez http://hdl.handle.net/20.500.12110/paper_03914097_v22_n6_p436_Alvarez |
| _version_ |
1768546304512753664 |