β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion
The effects of addition of divalent cations and phosphate buffer on the degree of β-carotene encapsulation in a mannitol matrix during freeze-drying were analyzed. The degradation rate of encapsulated β-carotene as a function of % RH and its relationship with the physical state of the matrix during...
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2007
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paper:paper_03785173_v332_n1-2_p45_Sutter2023-06-08T15:40:22Z β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion Buera, María del Pilar Elizalde, Beatriz Emilia β-Carotene Amorphous-state Crystallization inhibitor Encapsulation Mannitol crystallization Storage stability beta carotene buffer divalent cation mannitol phosphate article color crystallization differential scanning calorimetry drug degradation drug stability encapsulation freeze drying glass transition temperature hydration hydrogen bond priority journal storage temperature surface property water content Anions beta Carotene Buffers Cations, Divalent Chemistry, Pharmaceutical Color Crystallization Drug Compounding Drug Stability Drug Storage Excipients Freeze Drying Glycine Humidity Kinetics Mannitol Models, Chemical Phosphates Potassium Compounds Surface Properties Technology, Pharmaceutical Vitamins Water The effects of addition of divalent cations and phosphate buffer on the degree of β-carotene encapsulation in a mannitol matrix during freeze-drying were analyzed. The degradation rate of encapsulated β-carotene as a function of % RH and its relationship with the physical state of the matrix during storage at 25 °C was also studied. The presence of phosphate salts significantly delayed mannitol crystallization at a highly satisfactory degree during freeze-drying and, consequently, the degree of β-carotene encapsulation increased. This effect was maintained over quite long time during storage of the freeze-dried samples at 25 °C. Unavoidable local variations in water content during 3 years storage caused the decrease of Tg values and made the crystallization degree to increase. The divalent cations showed a synergistic effect and also modified the kinetics of β-carotene degradation during storage, increasing its stability. The mechanism of crystallization inhibition likely includes a change in hydrogen bond network or/and change in molecular mobility in the presence of divalent cations and phosphate anions. The degradation rate of β-carotene in a mannitol/KH2PO4 matrix increased as increasing % RH until a value at which the samples collapsed (75% RH), and then the degradation rate decreased. Collapse phenomena may affect diffusion of oxygen from the surface to the inside of the matrix and increase retention of β-carotene. Surface color was not an appropriate indicator for β-carotene degradation, because it was mostly dependent on the optical properties of the matrix, which changed with the degree of matrix hydration and collapse. © 2006 Elsevier B.V. All rights reserved. Fil:Buera, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Elizalde, B.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03785173_v332_n1-2_p45_Sutter http://hdl.handle.net/20.500.12110/paper_03785173_v332_n1-2_p45_Sutter |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Carotene Amorphous-state Crystallization inhibitor Encapsulation Mannitol crystallization Storage stability beta carotene buffer divalent cation mannitol phosphate article color crystallization differential scanning calorimetry drug degradation drug stability encapsulation freeze drying glass transition temperature hydration hydrogen bond priority journal storage temperature surface property water content Anions beta Carotene Buffers Cations, Divalent Chemistry, Pharmaceutical Color Crystallization Drug Compounding Drug Stability Drug Storage Excipients Freeze Drying Glycine Humidity Kinetics Mannitol Models, Chemical Phosphates Potassium Compounds Surface Properties Technology, Pharmaceutical Vitamins Water |
| spellingShingle |
β-Carotene Amorphous-state Crystallization inhibitor Encapsulation Mannitol crystallization Storage stability beta carotene buffer divalent cation mannitol phosphate article color crystallization differential scanning calorimetry drug degradation drug stability encapsulation freeze drying glass transition temperature hydration hydrogen bond priority journal storage temperature surface property water content Anions beta Carotene Buffers Cations, Divalent Chemistry, Pharmaceutical Color Crystallization Drug Compounding Drug Stability Drug Storage Excipients Freeze Drying Glycine Humidity Kinetics Mannitol Models, Chemical Phosphates Potassium Compounds Surface Properties Technology, Pharmaceutical Vitamins Water Buera, María del Pilar Elizalde, Beatriz Emilia β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| topic_facet |
β-Carotene Amorphous-state Crystallization inhibitor Encapsulation Mannitol crystallization Storage stability beta carotene buffer divalent cation mannitol phosphate article color crystallization differential scanning calorimetry drug degradation drug stability encapsulation freeze drying glass transition temperature hydration hydrogen bond priority journal storage temperature surface property water content Anions beta Carotene Buffers Cations, Divalent Chemistry, Pharmaceutical Color Crystallization Drug Compounding Drug Stability Drug Storage Excipients Freeze Drying Glycine Humidity Kinetics Mannitol Models, Chemical Phosphates Potassium Compounds Surface Properties Technology, Pharmaceutical Vitamins Water |
| description |
The effects of addition of divalent cations and phosphate buffer on the degree of β-carotene encapsulation in a mannitol matrix during freeze-drying were analyzed. The degradation rate of encapsulated β-carotene as a function of % RH and its relationship with the physical state of the matrix during storage at 25 °C was also studied. The presence of phosphate salts significantly delayed mannitol crystallization at a highly satisfactory degree during freeze-drying and, consequently, the degree of β-carotene encapsulation increased. This effect was maintained over quite long time during storage of the freeze-dried samples at 25 °C. Unavoidable local variations in water content during 3 years storage caused the decrease of Tg values and made the crystallization degree to increase. The divalent cations showed a synergistic effect and also modified the kinetics of β-carotene degradation during storage, increasing its stability. The mechanism of crystallization inhibition likely includes a change in hydrogen bond network or/and change in molecular mobility in the presence of divalent cations and phosphate anions. The degradation rate of β-carotene in a mannitol/KH2PO4 matrix increased as increasing % RH until a value at which the samples collapsed (75% RH), and then the degradation rate decreased. Collapse phenomena may affect diffusion of oxygen from the surface to the inside of the matrix and increase retention of β-carotene. Surface color was not an appropriate indicator for β-carotene degradation, because it was mostly dependent on the optical properties of the matrix, which changed with the degree of matrix hydration and collapse. © 2006 Elsevier B.V. All rights reserved. |
| author |
Buera, María del Pilar Elizalde, Beatriz Emilia |
| author_facet |
Buera, María del Pilar Elizalde, Beatriz Emilia |
| author_sort |
Buera, María del Pilar |
| title |
β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| title_short |
β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| title_full |
β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| title_fullStr |
β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| title_full_unstemmed |
β-Carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| title_sort |
β-carotene encapsulation in a mannitol matrix as affected by divalent cations and phosphate anion |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03785173_v332_n1-2_p45_Sutter http://hdl.handle.net/20.500.12110/paper_03785173_v332_n1-2_p45_Sutter |
| work_keys_str_mv |
AT bueramariadelpilar bcaroteneencapsulationinamannitolmatrixasaffectedbydivalentcationsandphosphateanion AT elizaldebeatrizemilia bcaroteneencapsulationinamannitolmatrixasaffectedbydivalentcationsandphosphateanion |
| _version_ |
1768546213551931392 |