Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy
Interleukin 1 (IL-1) is possibly one factor produced by the embryo that might have a role in the maternal immunological recognition of pregnancy. The purpose of this study was to identify an embryo-related factor suitable for prediction of pregnancy during IVF procedures. For this purpose, IL-1β lev...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03783782_v48_n1-2_p71_Baranao http://hdl.handle.net/20.500.12110/paper_03783782_v48_n1-2_p71_Baranao |
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paper:paper_03783782_v48_n1-2_p71_Baranao2023-06-08T15:39:41Z Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy Embryo culture-conditioned medium Interleukin 1β IVF Pregnancy interleukin 1beta antigen recognition article cell proliferation controlled study culture medium dna synthesis embryo embryo culture enzyme linked immunosorbent assay fertilization in vitro human prediction pregnancy supernatant thymocyte Culture Media, Conditioned Culture Techniques Embryo Embryo Transfer Female Fertilization in Vitro Humans Interleukin-1 Pregnancy Prospective Studies Interleukin 1 (IL-1) is possibly one factor produced by the embryo that might have a role in the maternal immunological recognition of pregnancy. The purpose of this study was to identify an embryo-related factor suitable for prediction of pregnancy during IVF procedures. For this purpose, IL-1β levels were measured in 21 samples of human embryo culture-conditioned media. The average number of embryos per sample was 5 ± 1. Simultaneously, 16 cell culture media containing 10% autologous serum but no embryos were tested as controls. IL-1β levels were measured using the ELISA technique, and the biological activity of IL-1 was measured by means of a C3H/HeJ mice thymocyte proliferation assay. The average IL-1β level ± S.E.M. was 49 ± 7 pg/ml in embryo culture-conditioned media and 12 ± 2 pg/ml in controls (P < 0.001). The average IL-1β level in embryo culture-conditioned media from viable pregnancy cycles was 82 ± 6 pg/ml (n = 8), while in those cases that did not result in viable pregnancies the IL-1β level was significantly lower (28 ± 4 pg/ml, (n = 13, P < 0.001). The IL-1 activity of embryo culture-conditioned media, measured by [3H]thymidine incorporation in thymocytes was increased, compared with control media (442 ± 51 counts/min vs. 337 ± 13 counts/min, P < 0.05), and the highest values corresponded to media containing those embryos that resulted in pregnancies (589 ± 41 counts/min, P < 0.01 vs. controls). We conclude that the determination of the levels of this cytokine in embryo culture-conditioned media might be a predictive parameter for pregnancies in patients undergoing IVF-ET. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03783782_v48_n1-2_p71_Baranao http://hdl.handle.net/20.500.12110/paper_03783782_v48_n1-2_p71_Baranao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Embryo culture-conditioned medium Interleukin 1β IVF Pregnancy interleukin 1beta antigen recognition article cell proliferation controlled study culture medium dna synthesis embryo embryo culture enzyme linked immunosorbent assay fertilization in vitro human prediction pregnancy supernatant thymocyte Culture Media, Conditioned Culture Techniques Embryo Embryo Transfer Female Fertilization in Vitro Humans Interleukin-1 Pregnancy Prospective Studies |
| spellingShingle |
Embryo culture-conditioned medium Interleukin 1β IVF Pregnancy interleukin 1beta antigen recognition article cell proliferation controlled study culture medium dna synthesis embryo embryo culture enzyme linked immunosorbent assay fertilization in vitro human prediction pregnancy supernatant thymocyte Culture Media, Conditioned Culture Techniques Embryo Embryo Transfer Female Fertilization in Vitro Humans Interleukin-1 Pregnancy Prospective Studies Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| topic_facet |
Embryo culture-conditioned medium Interleukin 1β IVF Pregnancy interleukin 1beta antigen recognition article cell proliferation controlled study culture medium dna synthesis embryo embryo culture enzyme linked immunosorbent assay fertilization in vitro human prediction pregnancy supernatant thymocyte Culture Media, Conditioned Culture Techniques Embryo Embryo Transfer Female Fertilization in Vitro Humans Interleukin-1 Pregnancy Prospective Studies |
| description |
Interleukin 1 (IL-1) is possibly one factor produced by the embryo that might have a role in the maternal immunological recognition of pregnancy. The purpose of this study was to identify an embryo-related factor suitable for prediction of pregnancy during IVF procedures. For this purpose, IL-1β levels were measured in 21 samples of human embryo culture-conditioned media. The average number of embryos per sample was 5 ± 1. Simultaneously, 16 cell culture media containing 10% autologous serum but no embryos were tested as controls. IL-1β levels were measured using the ELISA technique, and the biological activity of IL-1 was measured by means of a C3H/HeJ mice thymocyte proliferation assay. The average IL-1β level ± S.E.M. was 49 ± 7 pg/ml in embryo culture-conditioned media and 12 ± 2 pg/ml in controls (P < 0.001). The average IL-1β level in embryo culture-conditioned media from viable pregnancy cycles was 82 ± 6 pg/ml (n = 8), while in those cases that did not result in viable pregnancies the IL-1β level was significantly lower (28 ± 4 pg/ml, (n = 13, P < 0.001). The IL-1 activity of embryo culture-conditioned media, measured by [3H]thymidine incorporation in thymocytes was increased, compared with control media (442 ± 51 counts/min vs. 337 ± 13 counts/min, P < 0.05), and the highest values corresponded to media containing those embryos that resulted in pregnancies (589 ± 41 counts/min, P < 0.01 vs. controls). We conclude that the determination of the levels of this cytokine in embryo culture-conditioned media might be a predictive parameter for pregnancies in patients undergoing IVF-ET. |
| title |
Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| title_short |
Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| title_full |
Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| title_fullStr |
Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| title_full_unstemmed |
Interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| title_sort |
interleukin-1β levels in human embryo culture supernatants and their predictive value for pregnancy |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03783782_v48_n1-2_p71_Baranao http://hdl.handle.net/20.500.12110/paper_03783782_v48_n1-2_p71_Baranao |
| _version_ |
1768544368707239936 |