Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress

To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UT...

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Autores principales:	Rocchetta, Iara, Conforti, Visitación Teresa Dora
Publicado:	2007
Materias:	cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Darkness DNA, Algal DNA, Complementary DNA, Protozoan Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Plantae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v389_n2_p136_dosSantosFerreira http://hdl.handle.net/20.500.12110/paper_03781119_v389_n2_p136_dosSantosFerreira
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781119_v389_n2_p136_dosSantosFerreira
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spelling	paper:paper_03781119_v389_n2_p136_dosSantosFerreira2023-06-08T15:39:15Z Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress Rocchetta, Iara Conforti, Visitación Teresa Dora cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response. © 2006 Elsevier B.V. All rights reserved. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Conforti, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v389_n2_p136_dosSantosFerreira http://hdl.handle.net/20.500.12110/paper_03781119_v389_n2_p136_dosSantosFerreira
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae
spellingShingle	cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae Rocchetta, Iara Conforti, Visitación Teresa Dora Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
topic_facet	cDNA library Chromium EST Microarray Streptomycin chromium complementary DNA streptomycin article DNA microarray DNA sequence environmental stress Euglena gracilis expressed sequence tag gene expression nonhuman nucleotide sequence priority journal Animals Chromium Darkness DNA, Algal DNA, Complementary DNA, Protozoan Euglena gracilis Expressed Sequence Tags Gene Expression Profiling Gene Expression Regulation Gene Library Genes, Protozoan Streptomycin Euglena gracilis Plantae
description	To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response. © 2006 Elsevier B.V. All rights reserved.
author	Rocchetta, Iara Conforti, Visitación Teresa Dora
author_facet	Rocchetta, Iara Conforti, Visitación Teresa Dora
author_sort	Rocchetta, Iara
title	Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_short	Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_full	Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_fullStr	Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_full_unstemmed	Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_sort	gene expression patterns in euglena gracilis: insights into the cellular response to environmental stress
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v389_n2_p136_dosSantosFerreira http://hdl.handle.net/20.500.12110/paper_03781119_v389_n2_p136_dosSantosFerreira
work_keys_str_mv	AT rocchettaiara geneexpressionpatternsineuglenagracilisinsightsintothecellularresponsetoenvironmentalstress AT confortivisitacionteresadora geneexpressionpatternsineuglenagracilisinsightsintothecellularresponsetoenvironmentalstress
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Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress

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