A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens

A chromosomal region from Agrobacterium tumefaciens that complements exoC (pgm) mutations was cloned and sequenced. A cluster of three open reading frames (ORF1, ORF2 and ORF3) was identified. These genes are oriented in the same direction and are involved in the synthesis of glycogen and other poly...

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Autores principales:	Uttaro, Antonio Domingo, Ugalde, Rodolfo Augusto
Publicado:	1994
Materias:	cyclic β(l-2) glucan exoC mutants exopolysaccharides glycogen Nucleotide sequence succinoglycan exopolysaccharide glucan phosphomannomutase unclassified drug amino acid sequence article enzyme activity gene cluster gene mapping mutant nonhuman nucleotide sequence priority journal Rhizobium radiobacter sequence homology Agrobacterium tumefaciens Amino Acid Sequence Animal Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Transposable Elements DNA, Bacterial Genes, Bacterial Genes, Structural, Bacterial Molecular Sequence Data Multigene Family Mutation Nucleotidyltransferases Open Reading Frames Phosphoglucomutase Plasmids Rabbits Restriction Mapping Sequence Homology, Amino Acid Escherichia coli Oryctolagus cuniculus Xanthomonas campestris
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v150_n1_p117_Uttaro http://hdl.handle.net/20.500.12110/paper_03781119_v150_n1_p117_Uttaro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781119_v150_n1_p117_Uttaro
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spelling	paper:paper_03781119_v150_n1_p117_Uttaro2023-06-08T15:39:13Z A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens Uttaro, Antonio Domingo Ugalde, Rodolfo Augusto cyclic β(l-2) glucan exoC mutants exopolysaccharides glycogen Nucleotide sequence succinoglycan exopolysaccharide glucan phosphomannomutase succinoglycan unclassified drug amino acid sequence article enzyme activity gene cluster gene mapping mutant nonhuman nucleotide sequence priority journal Rhizobium radiobacter sequence homology Agrobacterium tumefaciens Amino Acid Sequence Animal Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Transposable Elements DNA, Bacterial Genes, Bacterial Genes, Structural, Bacterial Molecular Sequence Data Multigene Family Mutation Nucleotidyltransferases Open Reading Frames Phosphoglucomutase Plasmids Rabbits Restriction Mapping Sequence Homology, Amino Acid Agrobacterium tumefaciens Escherichia coli Oryctolagus cuniculus Xanthomonas campestris A chromosomal region from Agrobacterium tumefaciens that complements exoC (pgm) mutations was cloned and sequenced. A cluster of three open reading frames (ORF1, ORF2 and ORF3) was identified. These genes are oriented in the same direction and are involved in the synthesis of glycogen and other polysaccharides. ORF1 encodes a 420-amino-acid (aa) protein with 55.9% homology to Escherichia coli GlgC (ADP-glucose synthetase, EC 2.7.7.27). ORF2 encodes a 480-aa protein with 42.2% homology to E. coli GlgA (glycogen synthase, EC 2.4.1.21). Based on Tn5 mutagenesis and protein homology, ORF3 was identified as the structural gene encoding phosphoglucomutase (Pgm, EC 2.7.5.1). ORF3 encodes a 542-aa protein with 52.6% homology to rabbit Pgm. There is no significant homology (less than 20%) to the Xanthomonas campestris XanA protein, which displays phosphomannomutase (Pmm) and Pgm activities [Koplin et al. J. Bacteriol 174 (1992) 191-199]. An A. tumefaciens pgm::Tn5 mutant retains Pmm activity. © 1994. Fil:Uttaro, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v150_n1_p117_Uttaro http://hdl.handle.net/20.500.12110/paper_03781119_v150_n1_p117_Uttaro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclic β(l-2) glucan exoC mutants exopolysaccharides glycogen Nucleotide sequence succinoglycan exopolysaccharide glucan phosphomannomutase succinoglycan unclassified drug amino acid sequence article enzyme activity gene cluster gene mapping mutant nonhuman nucleotide sequence priority journal Rhizobium radiobacter sequence homology Agrobacterium tumefaciens Amino Acid Sequence Animal Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Transposable Elements DNA, Bacterial Genes, Bacterial Genes, Structural, Bacterial Molecular Sequence Data Multigene Family Mutation Nucleotidyltransferases Open Reading Frames Phosphoglucomutase Plasmids Rabbits Restriction Mapping Sequence Homology, Amino Acid Agrobacterium tumefaciens Escherichia coli Oryctolagus cuniculus Xanthomonas campestris
spellingShingle	cyclic β(l-2) glucan exoC mutants exopolysaccharides glycogen Nucleotide sequence succinoglycan exopolysaccharide glucan phosphomannomutase succinoglycan unclassified drug amino acid sequence article enzyme activity gene cluster gene mapping mutant nonhuman nucleotide sequence priority journal Rhizobium radiobacter sequence homology Agrobacterium tumefaciens Amino Acid Sequence Animal Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Transposable Elements DNA, Bacterial Genes, Bacterial Genes, Structural, Bacterial Molecular Sequence Data Multigene Family Mutation Nucleotidyltransferases Open Reading Frames Phosphoglucomutase Plasmids Rabbits Restriction Mapping Sequence Homology, Amino Acid Agrobacterium tumefaciens Escherichia coli Oryctolagus cuniculus Xanthomonas campestris Uttaro, Antonio Domingo Ugalde, Rodolfo Augusto A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
topic_facet	cyclic β(l-2) glucan exoC mutants exopolysaccharides glycogen Nucleotide sequence succinoglycan exopolysaccharide glucan phosphomannomutase succinoglycan unclassified drug amino acid sequence article enzyme activity gene cluster gene mapping mutant nonhuman nucleotide sequence priority journal Rhizobium radiobacter sequence homology Agrobacterium tumefaciens Amino Acid Sequence Animal Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Transposable Elements DNA, Bacterial Genes, Bacterial Genes, Structural, Bacterial Molecular Sequence Data Multigene Family Mutation Nucleotidyltransferases Open Reading Frames Phosphoglucomutase Plasmids Rabbits Restriction Mapping Sequence Homology, Amino Acid Agrobacterium tumefaciens Escherichia coli Oryctolagus cuniculus Xanthomonas campestris
description	A chromosomal region from Agrobacterium tumefaciens that complements exoC (pgm) mutations was cloned and sequenced. A cluster of three open reading frames (ORF1, ORF2 and ORF3) was identified. These genes are oriented in the same direction and are involved in the synthesis of glycogen and other polysaccharides. ORF1 encodes a 420-amino-acid (aa) protein with 55.9% homology to Escherichia coli GlgC (ADP-glucose synthetase, EC 2.7.7.27). ORF2 encodes a 480-aa protein with 42.2% homology to E. coli GlgA (glycogen synthase, EC 2.4.1.21). Based on Tn5 mutagenesis and protein homology, ORF3 was identified as the structural gene encoding phosphoglucomutase (Pgm, EC 2.7.5.1). ORF3 encodes a 542-aa protein with 52.6% homology to rabbit Pgm. There is no significant homology (less than 20%) to the Xanthomonas campestris XanA protein, which displays phosphomannomutase (Pmm) and Pgm activities [Koplin et al. J. Bacteriol 174 (1992) 191-199]. An A. tumefaciens pgm::Tn5 mutant retains Pmm activity. © 1994.
author	Uttaro, Antonio Domingo Ugalde, Rodolfo Augusto
author_facet	Uttaro, Antonio Domingo Ugalde, Rodolfo Augusto
author_sort	Uttaro, Antonio Domingo
title	A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
title_short	A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
title_full	A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
title_fullStr	A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
title_full_unstemmed	A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
title_sort	chromosomal cluster of genes encoding adp-glucose synthetase, glycogen synthase and phosphoglucomutase in agrobacterium tumefaciens
publishDate	1994
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781119_v150_n1_p117_Uttaro http://hdl.handle.net/20.500.12110/paper_03781119_v150_n1_p117_Uttaro
work_keys_str_mv	AT uttaroantoniodomingo achromosomalclusterofgenesencodingadpglucosesynthetaseglycogensynthaseandphosphoglucomutaseinagrobacteriumtumefaciens AT ugalderodolfoaugusto achromosomalclusterofgenesencodingadpglucosesynthetaseglycogensynthaseandphosphoglucomutaseinagrobacteriumtumefaciens AT uttaroantoniodomingo chromosomalclusterofgenesencodingadpglucosesynthetaseglycogensynthaseandphosphoglucomutaseinagrobacteriumtumefaciens AT ugalderodolfoaugusto chromosomalclusterofgenesencodingadpglucosesynthetaseglycogensynthaseandphosphoglucomutaseinagrobacteriumtumefaciens
_version_	1768542271564677120

A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens

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