A plasmid vector for isolation of strong promoters in Escherichia coli

In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning...

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Autor principal:	Seigelchifer, Mauricio Angel
Publicado:	1999
Materias:	Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Negibacteria Posibacteria Prokaryota unidentified bacteriophage
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v177_n1_p75_Carbonelli http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781097_v177_n1_p75_Carbonelli
record_format	dspace
spelling	paper:paper_03781097_v177_n1_p75_Carbonelli2023-06-08T15:39:07Z A plasmid vector for isolation of strong promoters in Escherichia coli Seigelchifer, Mauricio Angel Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies. Fil:Seigelchifer, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v177_n1_p75_Carbonelli http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage
spellingShingle	Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage Seigelchifer, Mauricio Angel A plasmid vector for isolation of strong promoters in Escherichia coli
topic_facet	Bacterial promoter Bacteriophage promoter Promoter isolation Strong promoter Vector ampicillin DNA fragment penicillinase plasmid vector tetracycline antibiotic resistance article bacteriophage DNA sequence Escherichia coli nonhuman nucleotide sequence penicillin resistance plasmid priority journal promoter region Staphylococcus aureus Bacteria (microorganisms) bacteriophage a Escherichia coli Escherichia coli Negibacteria Posibacteria Prokaryota Staphylococcus aureus Staphylococcus aureus unidentified bacteriophage
description	In order to isolate very strong promoters from bacteria and bacteriophage a plasmid named pProm was constructed. It possesses an origin (ORI) for replication in Gram-negative bacteria, an ORI for replication in Gram-positive bacteria, a promoterless ampicillin resistance gene with a multiple cloning site (MCS) in the position formerly occupied by the ampicillin promoter, a tetracycline resistance gene for selection in Gram-negative bacteria and a chloramphenicol resistance gene for selection in Gram-positive bacteria. Insertion in the MCS of DNA fragments of Staphylococcus aureus bacteriophages resulted in isolation of several clones very resistant to ampicillin. The DNA fragments inserted in these recombinant plasmids were sequenced and all of them contained putative promoter motifs. Direct measurement of the penicillinase activity indicated that one of the isolated promoters could be included within a group of the stronger known prokaryotic promoters. According to these results pProm is a powerful tool to perform studies on promoter strength and for industrial applications. Copyright (C) 1999 Federation of European Microbiological Societies.
author	Seigelchifer, Mauricio Angel
author_facet	Seigelchifer, Mauricio Angel
author_sort	Seigelchifer, Mauricio Angel
title	A plasmid vector for isolation of strong promoters in Escherichia coli
title_short	A plasmid vector for isolation of strong promoters in Escherichia coli
title_full	A plasmid vector for isolation of strong promoters in Escherichia coli
title_fullStr	A plasmid vector for isolation of strong promoters in Escherichia coli
title_full_unstemmed	A plasmid vector for isolation of strong promoters in Escherichia coli
title_sort	plasmid vector for isolation of strong promoters in escherichia coli
publishDate	1999
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v177_n1_p75_Carbonelli http://hdl.handle.net/20.500.12110/paper_03781097_v177_n1_p75_Carbonelli
work_keys_str_mv	AT seigelchifermauricioangel aplasmidvectorforisolationofstrongpromotersinescherichiacoli AT seigelchifermauricioangel plasmidvectorforisolationofstrongpromotersinescherichiacoli
_version_	1768541799970766848

A plasmid vector for isolation of strong promoters in Escherichia coli

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