Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23

The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repr...

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Autor principal:	Rubinstein, Clara Patricia
Publicado:	1998
Materias:	Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v167_n2_p315_Rubinstein http://hdl.handle.net/20.500.12110/paper_03781097_v167_n2_p315_Rubinstein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781097_v167_n2_p315_Rubinstein
record_format	dspace
spelling	paper:paper_03781097_v167_n2_p315_Rubinstein2025-07-30T18:15:47Z Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23 Rubinstein, Clara Patricia Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repressor reduced the survival capacity of the host cells after mitomycin C or nalidixic acid treatments and lowered the spontaneous reversion frequency. When SOS induction kinetics were studied, low or null induction of the damage-inducible din22::LacZ fusion was observed. In contrast, the presence of the wild-type prophage amplified the SOS response. Sequencing of the mutant repressor revealed that the cts23 mutation is a T→C transition affecting the 5' closest codon to one of the two reported DNA binding domains. Copyright (C) 1998 Federation of European Microbiological Societies. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v167_n2_p315_Rubinstein http://hdl.handle.net/20.500.12110/paper_03781097_v167_n2_p315_Rubinstein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis
spellingShingle	Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis Rubinstein, Clara Patricia Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
topic_facet	Bacillus subtilis Bacteriophage φ105 Repair SOS induction Thermosensitive repressor article bacillus subtilis bacteriophage dna repair heat sensitivity mutant nonhuman priority journal Artificial Gene Fusion Bacillus Phages Bacillus subtilis beta-Galactosidase DNA Damage DNA Repair Gene Expression Regulation, Bacterial Genes, Viral Mutation Plasmids Repressor Proteins SOS Response (Genetics) Temperature Viral Proteins Bacillus subtilis
description	The presence of the Φ105cts23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti-SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts23 mutant repressor was cloned and sequenced. The isolated repressor reduced the survival capacity of the host cells after mitomycin C or nalidixic acid treatments and lowered the spontaneous reversion frequency. When SOS induction kinetics were studied, low or null induction of the damage-inducible din22::LacZ fusion was observed. In contrast, the presence of the wild-type prophage amplified the SOS response. Sequencing of the mutant repressor revealed that the cts23 mutation is a T→C transition affecting the 5' closest codon to one of the two reported DNA binding domains. Copyright (C) 1998 Federation of European Microbiological Societies.
author	Rubinstein, Clara Patricia
author_facet	Rubinstein, Clara Patricia
author_sort	Rubinstein, Clara Patricia
title	Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
title_short	Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
title_full	Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
title_fullStr	Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
title_full_unstemmed	Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
title_sort	normal induction of the sos response in bacillus subtilis is prevented by the mutant repressor from phage φ105cts23
publishDate	1998
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v167_n2_p315_Rubinstein http://hdl.handle.net/20.500.12110/paper_03781097_v167_n2_p315_Rubinstein
work_keys_str_mv	AT rubinsteinclarapatricia normalinductionofthesosresponseinbacillussubtilisispreventedbythemutantrepressorfromphageph105cts23
_version_	1840322525901357056

Normal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105cts23

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