Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex

The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel elec...

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Autor principal:	Azcurra, Julio Marcos
Publicado:	1978
Materias:	rna animal experiment brain cortex central nervous system nerve cell nervous system rat Animal Cerebral Cortex Cytoplasm Electrophoresis, Polyacrylamide Gel Molecular Weight Rats RNA RNA, Ribosomal
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v3_n5_p521_Azcurra http://hdl.handle.net/20.500.12110/paper_03643190_v3_n5_p521_Azcurra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03643190_v3_n5_p521_Azcurra
record_format	dspace
spelling	paper:paper_03643190_v3_n5_p521_Azcurra2023-06-08T15:35:36Z Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex Azcurra, Julio Marcos rna animal experiment brain cortex central nervous system nerve cell nervous system rat Animal Cerebral Cortex Cytoplasm Electrophoresis, Polyacrylamide Gel Molecular Weight Rats RNA RNA, Ribosomal The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel electrophoresis and pulse labelling with 5-[3H]uridine were used to confirm the delayed appearance of newly synthesized rRNA in the cytoplasm and to demonstrate its stability. "Polydisperse" RNA appeared in the cytoplasm earlier and had a shorter half-life than rRNA. A wide range of molecular weights for this RNA was found with no predominant individual species. A set of cytoplasmic RNA components of molecular size between 28S and 18S was also present probably reflecting the in vivo degradation of rRNA. The significance of the unexpectedly high amounts of neuronal polydisperse RNA is discussed. © 1978 Plenum Publishing Corporation. Fil:Azcurra, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v3_n5_p521_Azcurra http://hdl.handle.net/20.500.12110/paper_03643190_v3_n5_p521_Azcurra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	rna animal experiment brain cortex central nervous system nerve cell nervous system rat Animal Cerebral Cortex Cytoplasm Electrophoresis, Polyacrylamide Gel Molecular Weight Rats RNA RNA, Ribosomal
spellingShingle	rna animal experiment brain cortex central nervous system nerve cell nervous system rat Animal Cerebral Cortex Cytoplasm Electrophoresis, Polyacrylamide Gel Molecular Weight Rats RNA RNA, Ribosomal Azcurra, Julio Marcos Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
topic_facet	rna animal experiment brain cortex central nervous system nerve cell nervous system rat Animal Cerebral Cortex Cytoplasm Electrophoresis, Polyacrylamide Gel Molecular Weight Rats RNA RNA, Ribosomal
description	The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel electrophoresis and pulse labelling with 5-[3H]uridine were used to confirm the delayed appearance of newly synthesized rRNA in the cytoplasm and to demonstrate its stability. "Polydisperse" RNA appeared in the cytoplasm earlier and had a shorter half-life than rRNA. A wide range of molecular weights for this RNA was found with no predominant individual species. A set of cytoplasmic RNA components of molecular size between 28S and 18S was also present probably reflecting the in vivo degradation of rRNA. The significance of the unexpectedly high amounts of neuronal polydisperse RNA is discussed. © 1978 Plenum Publishing Corporation.
author	Azcurra, Julio Marcos
author_facet	Azcurra, Julio Marcos
author_sort	Azcurra, Julio Marcos
title	Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_short	Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_full	Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_fullStr	Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_full_unstemmed	Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
title_sort	identification of polydisperse rna in the cytoplasm of neurons isolated from the immature brain cortex
publishDate	1978
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v3_n5_p521_Azcurra http://hdl.handle.net/20.500.12110/paper_03643190_v3_n5_p521_Azcurra
work_keys_str_mv	AT azcurrajuliomarcos identificationofpolydispersernainthecytoplasmofneuronsisolatedfromtheimmaturebraincortex
_version_	1768543231028494336

Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex

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