B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training
Several lines of evidence indicate that protein kinase C (PKC) is involved in long-term potentiation (LTP) and in certain forms of learning. Recently, we found a learning-specific, time-dependent increase in [3H]phorbol dibutyrate binding to membrane-associated PKC in the hippocampus of rats subject...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v22_n4_p499_Cammarota http://hdl.handle.net/20.500.12110/paper_03643190_v22_n4_p499_Cammarota |
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paper:paper_03643190_v22_n4_p499_Cammarota2023-06-08T15:35:34Z B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training Cammarota, Martín Pablo Paratcha, Gustavo Christian Bernabeu, Ramón Oscar B-50/GAP-43 hippocampus learning memory PKC protein phosphorylation 1 (5 isoquinolinesulfonyl) 2 methylpiperazine neuromodulin phorbol dibutyrate polymyxin B protein kinase C protein kinase C inhibitor staurosporine derivative tritium animal cell animal experiment article brain synaptosome controlled study enzyme activity hippocampus learning long term potentiation male memory nonhuman priority journal protein phosphorylation rat synaptic membrane task performance training Animalia Bovinae Tritium Several lines of evidence indicate that protein kinase C (PKC) is involved in long-term potentiation (LTP) and in certain forms of learning. Recently, we found a learning-specific, time-dependent increase in [3H]phorbol dibutyrate binding to membrane-associated PKC in the hippocampus of rats subjected to an inhibitory avoidance task. Here we confirm and extend this observation, describing that a one trial inhibitory avoidance learning was associated with rapid and specific increases in B-50/GAP-43 phosphorylation in vitro and in PKC activity in hippocampal synaptosomal membranes. The increased phosphorylation of B-50/GAP-43 was seen at 30 rain (+35% relative to naive or shocked control groups), but not at 10 or 60 rain after training. This learning-associated increase in the phosphorylation of B-50/GAP-43 is mainly due to an increase in the activity of PKC. This is based on three different sets of data: 1) PKC activity increased by 24% in hippocampal synaptosomal membranes of rats sacrificed 30 min after training; 2) B-50/GAP-43 immunoblots revealed no changes in the amount of this protein among the different experimental groups; 3) phosphorylation assays, performed in the presence of bovine purified PKC or in the presence of the selective PKC inhibitor CGP 41231, exhibited no differences in B-50/GAP-43 phosphorylation between naive and trained animals. In conclusion, these results support the contention that hippocampal PKC participates in the early neural events of memory formation of an aversively-motivated learning task. Fil:Cammarota, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paratcha, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bernabeu, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v22_n4_p499_Cammarota http://hdl.handle.net/20.500.12110/paper_03643190_v22_n4_p499_Cammarota |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
B-50/GAP-43 hippocampus learning memory PKC protein phosphorylation 1 (5 isoquinolinesulfonyl) 2 methylpiperazine neuromodulin phorbol dibutyrate polymyxin B protein kinase C protein kinase C inhibitor staurosporine derivative tritium animal cell animal experiment article brain synaptosome controlled study enzyme activity hippocampus learning long term potentiation male memory nonhuman priority journal protein phosphorylation rat synaptic membrane task performance training Animalia Bovinae Tritium |
| spellingShingle |
B-50/GAP-43 hippocampus learning memory PKC protein phosphorylation 1 (5 isoquinolinesulfonyl) 2 methylpiperazine neuromodulin phorbol dibutyrate polymyxin B protein kinase C protein kinase C inhibitor staurosporine derivative tritium animal cell animal experiment article brain synaptosome controlled study enzyme activity hippocampus learning long term potentiation male memory nonhuman priority journal protein phosphorylation rat synaptic membrane task performance training Animalia Bovinae Tritium Cammarota, Martín Pablo Paratcha, Gustavo Christian Bernabeu, Ramón Oscar B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| topic_facet |
B-50/GAP-43 hippocampus learning memory PKC protein phosphorylation 1 (5 isoquinolinesulfonyl) 2 methylpiperazine neuromodulin phorbol dibutyrate polymyxin B protein kinase C protein kinase C inhibitor staurosporine derivative tritium animal cell animal experiment article brain synaptosome controlled study enzyme activity hippocampus learning long term potentiation male memory nonhuman priority journal protein phosphorylation rat synaptic membrane task performance training Animalia Bovinae Tritium |
| description |
Several lines of evidence indicate that protein kinase C (PKC) is involved in long-term potentiation (LTP) and in certain forms of learning. Recently, we found a learning-specific, time-dependent increase in [3H]phorbol dibutyrate binding to membrane-associated PKC in the hippocampus of rats subjected to an inhibitory avoidance task. Here we confirm and extend this observation, describing that a one trial inhibitory avoidance learning was associated with rapid and specific increases in B-50/GAP-43 phosphorylation in vitro and in PKC activity in hippocampal synaptosomal membranes. The increased phosphorylation of B-50/GAP-43 was seen at 30 rain (+35% relative to naive or shocked control groups), but not at 10 or 60 rain after training. This learning-associated increase in the phosphorylation of B-50/GAP-43 is mainly due to an increase in the activity of PKC. This is based on three different sets of data: 1) PKC activity increased by 24% in hippocampal synaptosomal membranes of rats sacrificed 30 min after training; 2) B-50/GAP-43 immunoblots revealed no changes in the amount of this protein among the different experimental groups; 3) phosphorylation assays, performed in the presence of bovine purified PKC or in the presence of the selective PKC inhibitor CGP 41231, exhibited no differences in B-50/GAP-43 phosphorylation between naive and trained animals. In conclusion, these results support the contention that hippocampal PKC participates in the early neural events of memory formation of an aversively-motivated learning task. |
| author |
Cammarota, Martín Pablo Paratcha, Gustavo Christian Bernabeu, Ramón Oscar |
| author_facet |
Cammarota, Martín Pablo Paratcha, Gustavo Christian Bernabeu, Ramón Oscar |
| author_sort |
Cammarota, Martín Pablo |
| title |
B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| title_short |
B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| title_full |
B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| title_fullStr |
B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| title_full_unstemmed |
B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| title_sort |
b-50/gap-43 phosphorylation and pkc activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v22_n4_p499_Cammarota http://hdl.handle.net/20.500.12110/paper_03643190_v22_n4_p499_Cammarota |
| work_keys_str_mv |
AT cammarotamartinpablo b50gap43phosphorylationandpkcactivityareincreasedinrathippocampalsynaptosomalmembranesafteraninhibitoryavoidancetraining AT paratchagustavochristian b50gap43phosphorylationandpkcactivityareincreasedinrathippocampalsynaptosomalmembranesafteraninhibitoryavoidancetraining AT bernabeuramonoscar b50gap43phosphorylationandpkcactivityareincreasedinrathippocampalsynaptosomalmembranesafteraninhibitoryavoidancetraining |
| _version_ |
1768542178758361088 |