Effect of transferrin on hypomyelination induced by iron deficiency
We have used a model of iron deficiency in the rat to analyze the effects of a disruption in iron availability on oligodendroglial cell (OLGc) maturation and myelinogenesis and to explore the possible beneficial influence of an intracranial injection (ICI) of apotransferrin (aTf) at 3 days of age on...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v86_n12_p2663_Badaracco http://hdl.handle.net/20.500.12110/paper_03604012_v86_n12_p2663_Badaracco |
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paper:paper_03604012_v86_n12_p2663_Badaracco2023-06-08T15:34:46Z Effect of transferrin on hypomyelination induced by iron deficiency Ferritin Hypomyelination Iron deficiency Oligodendroglial cells Transferrin APC protein apotransferrin ferritin galactolipid myelin basic protein myelin protein nerve cell adhesion molecule phospholipid prion protein antibody animal experiment animal model animal tissue article brain weight cell maturation controlled study immunocytochemistry immunohistochemistry iron deficiency iron restriction lipid raft myelin sheath myelination nonhuman oligodendroglia priority journal rat remyelinization Anemia, Iron-Deficiency Animals Animals, Newborn Apoproteins Cells, Cultured Demyelinating Diseases Disease Models, Animal Female Myelin Sheath Nerve Fibers, Myelinated Pregnancy Rats Rats, Wistar Transferrin We have used a model of iron deficiency in the rat to analyze the effects of a disruption in iron availability on oligodendroglial cell (OLGc) maturation and myelinogenesis and to explore the possible beneficial influence of an intracranial injection (ICI) of apotransferrin (aTf) at 3 days of age on this process. Studies carried out on postnatal days 17 and 24 showed that iron deficiency produced a decrease in myelin proteins and lipids at 24 days of age. Immunohistochemistry showed that in untreated iron-deficient (ID) rats, the immunoreactivity of anti-adenomatous polyposis coli (APC) and anti-MBP antibodies decreased markedly with reference to normal controls, whereas in ID rats treated with an ICI of aTf, the immunoreactivity of these markers increased. A similar situation occurred with the immunoreactivity of H-ferritin. In primary OLGc cultures from ID rats, there was a high number of cells positive to the antibody against the polysialylated form of the cell surface glycoprotein NCAM (PSA-NCAM) compared with in OLGc cultures prepared from normal controls or from ID animals treated with aTf. The number of MBP+ cells in cultures from ID rats increased after treatment with aTf. The presence of lipid rafts evaluated with a specific anti-protein prion cellular (PrPc) antibody showed a smaller number of PrPc-positive structures in ID rat cultures. Treatment of the ID animals with a single ICI of aTf stimulated myelination, producing a significant correction in the different biochemical parameters affected by ID. © 2008 Wiley-Liss, Inc. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v86_n12_p2663_Badaracco http://hdl.handle.net/20.500.12110/paper_03604012_v86_n12_p2663_Badaracco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ferritin Hypomyelination Iron deficiency Oligodendroglial cells Transferrin APC protein apotransferrin ferritin galactolipid myelin basic protein myelin protein nerve cell adhesion molecule phospholipid prion protein antibody animal experiment animal model animal tissue article brain weight cell maturation controlled study immunocytochemistry immunohistochemistry iron deficiency iron restriction lipid raft myelin sheath myelination nonhuman oligodendroglia priority journal rat remyelinization Anemia, Iron-Deficiency Animals Animals, Newborn Apoproteins Cells, Cultured Demyelinating Diseases Disease Models, Animal Female Myelin Sheath Nerve Fibers, Myelinated Pregnancy Rats Rats, Wistar Transferrin |
| spellingShingle |
Ferritin Hypomyelination Iron deficiency Oligodendroglial cells Transferrin APC protein apotransferrin ferritin galactolipid myelin basic protein myelin protein nerve cell adhesion molecule phospholipid prion protein antibody animal experiment animal model animal tissue article brain weight cell maturation controlled study immunocytochemistry immunohistochemistry iron deficiency iron restriction lipid raft myelin sheath myelination nonhuman oligodendroglia priority journal rat remyelinization Anemia, Iron-Deficiency Animals Animals, Newborn Apoproteins Cells, Cultured Demyelinating Diseases Disease Models, Animal Female Myelin Sheath Nerve Fibers, Myelinated Pregnancy Rats Rats, Wistar Transferrin Effect of transferrin on hypomyelination induced by iron deficiency |
| topic_facet |
Ferritin Hypomyelination Iron deficiency Oligodendroglial cells Transferrin APC protein apotransferrin ferritin galactolipid myelin basic protein myelin protein nerve cell adhesion molecule phospholipid prion protein antibody animal experiment animal model animal tissue article brain weight cell maturation controlled study immunocytochemistry immunohistochemistry iron deficiency iron restriction lipid raft myelin sheath myelination nonhuman oligodendroglia priority journal rat remyelinization Anemia, Iron-Deficiency Animals Animals, Newborn Apoproteins Cells, Cultured Demyelinating Diseases Disease Models, Animal Female Myelin Sheath Nerve Fibers, Myelinated Pregnancy Rats Rats, Wistar Transferrin |
| description |
We have used a model of iron deficiency in the rat to analyze the effects of a disruption in iron availability on oligodendroglial cell (OLGc) maturation and myelinogenesis and to explore the possible beneficial influence of an intracranial injection (ICI) of apotransferrin (aTf) at 3 days of age on this process. Studies carried out on postnatal days 17 and 24 showed that iron deficiency produced a decrease in myelin proteins and lipids at 24 days of age. Immunohistochemistry showed that in untreated iron-deficient (ID) rats, the immunoreactivity of anti-adenomatous polyposis coli (APC) and anti-MBP antibodies decreased markedly with reference to normal controls, whereas in ID rats treated with an ICI of aTf, the immunoreactivity of these markers increased. A similar situation occurred with the immunoreactivity of H-ferritin. In primary OLGc cultures from ID rats, there was a high number of cells positive to the antibody against the polysialylated form of the cell surface glycoprotein NCAM (PSA-NCAM) compared with in OLGc cultures prepared from normal controls or from ID animals treated with aTf. The number of MBP+ cells in cultures from ID rats increased after treatment with aTf. The presence of lipid rafts evaluated with a specific anti-protein prion cellular (PrPc) antibody showed a smaller number of PrPc-positive structures in ID rat cultures. Treatment of the ID animals with a single ICI of aTf stimulated myelination, producing a significant correction in the different biochemical parameters affected by ID. © 2008 Wiley-Liss, Inc. |
| title |
Effect of transferrin on hypomyelination induced by iron deficiency |
| title_short |
Effect of transferrin on hypomyelination induced by iron deficiency |
| title_full |
Effect of transferrin on hypomyelination induced by iron deficiency |
| title_fullStr |
Effect of transferrin on hypomyelination induced by iron deficiency |
| title_full_unstemmed |
Effect of transferrin on hypomyelination induced by iron deficiency |
| title_sort |
effect of transferrin on hypomyelination induced by iron deficiency |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v86_n12_p2663_Badaracco http://hdl.handle.net/20.500.12110/paper_03604012_v86_n12_p2663_Badaracco |
| _version_ |
1768545052973334528 |