Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment

A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported...

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Autores principales:	Pettinari, María Julia, Tribelli, Paula María, Méndez, Beatriz Silvia
Publicado:	2009
Materias:	arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas veronii
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v59_n5_p514_Lopez http://hdl.handle.net/20.500.12110/paper_03438651_v59_n5_p514_Lopez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_03438651_v59_n5_p514_Lopez2023-06-08T15:34:33Z Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment Pettinari, María Julia Tribelli, Paula María Méndez, Beatriz Silvia arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, -galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37°C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM 17835T and as CIP 109839T. © 2009 Springer Science+Business Media, LLC. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v59_n5_p514_Lopez http://hdl.handle.net/20.500.12110/paper_03438651_v59_n5_p514_Lopez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii
spellingShingle	arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii Pettinari, María Julia Tribelli, Paula María Méndez, Beatriz Silvia Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
topic_facet	arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii
description	A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, -galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37°C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM 17835T and as CIP 109839T. © 2009 Springer Science+Business Media, LLC.
author	Pettinari, María Julia Tribelli, Paula María Méndez, Beatriz Silvia
author_facet	Pettinari, María Julia Tribelli, Paula María Méndez, Beatriz Silvia
author_sort	Pettinari, María Julia
title	Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
title_short	Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
title_full	Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
title_fullStr	Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
title_full_unstemmed	Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
title_sort	pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v59_n5_p514_Lopez http://hdl.handle.net/20.500.12110/paper_03438651_v59_n5_p514_Lopez
work_keys_str_mv	AT pettinarimariajulia pseudomonasextremaustralisspnovapoly3hydroxybutyrateproducerisolatedfromanantarcticenvironment AT tribellipaulamaria pseudomonasextremaustralisspnovapoly3hydroxybutyrateproducerisolatedfromanantarcticenvironment AT mendezbeatrizsilvia pseudomonasextremaustralisspnovapoly3hydroxybutyrateproducerisolatedfromanantarcticenvironment
_version_	1768546211109797888

Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment

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