Spatial-specific action of serotonin within the leech midbody ganglion
Serotonin is a conspicuous neuromodulator in the nervous system of many vertebrates and invertebrates. In previous experiments performed in the leech nervous system, we compared the effect of the amine released from endogenous sources [using selective serotonin reuptake inhibitors (SSRIs), e.g. fluo...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03407594_v194_n6_p523_Calvino http://hdl.handle.net/20.500.12110/paper_03407594_v194_n6_p523_Calvino |
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paper:paper_03407594_v194_n6_p523_Calvino2023-06-08T15:34:22Z Spatial-specific action of serotonin within the leech midbody ganglion Calviño, María Ana Szczupak, Lidia 5-HT antagonist Autoreceptor Interneuronal pathway Methiothepin Neuromodulation cyproheptadine fluoxetine metitepine serotonin serotonin antagonist serotonin uptake inhibitor animal article drug effect electrophysiology excitatory postsynaptic potential ganglion histology iontophoresis leech motoneuron nerve tract neuropil physiology statistical analysis Animals Cyproheptadine Data Interpretation, Statistical Electrophysiology Excitatory Postsynaptic Potentials Fluoxetine Ganglia, Invertebrate Iontophoresis Leeches Methiothepin Motor Neurons Neural Pathways Neuropil Serotonin Serotonin Antagonists Serotonin Uptake Inhibitors Serotonin is a conspicuous neuromodulator in the nervous system of many vertebrates and invertebrates. In previous experiments performed in the leech nervous system, we compared the effect of the amine released from endogenous sources [using selective serotonin reuptake inhibitors (SSRIs), e.g. fluoxetine] with that of bath-applied serotonin. The results suggested that the amine does not reach all its targets in a uniform way, but produces the activation of an interneuronal pathway that generated specific synaptic responses on different neurons. Taking into account that the release of the amine is often regulated at the presynaptic level, we have investigated whether autoreceptor antagonists mimic the SSRIs effect. We found that methiothepin (100 μM) produced similar effects than fluoxetine. To further test the hypothesis that endogenous serotonin produce its effect by acting locally at specific sites, we analyzed the effect of iontophoretic applications of serotonin. We found a site in the neuropil of the leech ganglia where serotonin application mimicked the effect of the SSRIs and the 5-HT antagonist. The results further support the view that the effect of serotonin exhibits a spatial specificity that can be relevant to understand its modulatory actions. © 2008 Springer-Verlag. Fil:Calviño, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Szczupak, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03407594_v194_n6_p523_Calvino http://hdl.handle.net/20.500.12110/paper_03407594_v194_n6_p523_Calvino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5-HT antagonist Autoreceptor Interneuronal pathway Methiothepin Neuromodulation cyproheptadine fluoxetine metitepine serotonin serotonin antagonist serotonin uptake inhibitor animal article drug effect electrophysiology excitatory postsynaptic potential ganglion histology iontophoresis leech motoneuron nerve tract neuropil physiology statistical analysis Animals Cyproheptadine Data Interpretation, Statistical Electrophysiology Excitatory Postsynaptic Potentials Fluoxetine Ganglia, Invertebrate Iontophoresis Leeches Methiothepin Motor Neurons Neural Pathways Neuropil Serotonin Serotonin Antagonists Serotonin Uptake Inhibitors |
| spellingShingle |
5-HT antagonist Autoreceptor Interneuronal pathway Methiothepin Neuromodulation cyproheptadine fluoxetine metitepine serotonin serotonin antagonist serotonin uptake inhibitor animal article drug effect electrophysiology excitatory postsynaptic potential ganglion histology iontophoresis leech motoneuron nerve tract neuropil physiology statistical analysis Animals Cyproheptadine Data Interpretation, Statistical Electrophysiology Excitatory Postsynaptic Potentials Fluoxetine Ganglia, Invertebrate Iontophoresis Leeches Methiothepin Motor Neurons Neural Pathways Neuropil Serotonin Serotonin Antagonists Serotonin Uptake Inhibitors Calviño, María Ana Szczupak, Lidia Spatial-specific action of serotonin within the leech midbody ganglion |
| topic_facet |
5-HT antagonist Autoreceptor Interneuronal pathway Methiothepin Neuromodulation cyproheptadine fluoxetine metitepine serotonin serotonin antagonist serotonin uptake inhibitor animal article drug effect electrophysiology excitatory postsynaptic potential ganglion histology iontophoresis leech motoneuron nerve tract neuropil physiology statistical analysis Animals Cyproheptadine Data Interpretation, Statistical Electrophysiology Excitatory Postsynaptic Potentials Fluoxetine Ganglia, Invertebrate Iontophoresis Leeches Methiothepin Motor Neurons Neural Pathways Neuropil Serotonin Serotonin Antagonists Serotonin Uptake Inhibitors |
| description |
Serotonin is a conspicuous neuromodulator in the nervous system of many vertebrates and invertebrates. In previous experiments performed in the leech nervous system, we compared the effect of the amine released from endogenous sources [using selective serotonin reuptake inhibitors (SSRIs), e.g. fluoxetine] with that of bath-applied serotonin. The results suggested that the amine does not reach all its targets in a uniform way, but produces the activation of an interneuronal pathway that generated specific synaptic responses on different neurons. Taking into account that the release of the amine is often regulated at the presynaptic level, we have investigated whether autoreceptor antagonists mimic the SSRIs effect. We found that methiothepin (100 μM) produced similar effects than fluoxetine. To further test the hypothesis that endogenous serotonin produce its effect by acting locally at specific sites, we analyzed the effect of iontophoretic applications of serotonin. We found a site in the neuropil of the leech ganglia where serotonin application mimicked the effect of the SSRIs and the 5-HT antagonist. The results further support the view that the effect of serotonin exhibits a spatial specificity that can be relevant to understand its modulatory actions. © 2008 Springer-Verlag. |
| author |
Calviño, María Ana Szczupak, Lidia |
| author_facet |
Calviño, María Ana Szczupak, Lidia |
| author_sort |
Calviño, María Ana |
| title |
Spatial-specific action of serotonin within the leech midbody ganglion |
| title_short |
Spatial-specific action of serotonin within the leech midbody ganglion |
| title_full |
Spatial-specific action of serotonin within the leech midbody ganglion |
| title_fullStr |
Spatial-specific action of serotonin within the leech midbody ganglion |
| title_full_unstemmed |
Spatial-specific action of serotonin within the leech midbody ganglion |
| title_sort |
spatial-specific action of serotonin within the leech midbody ganglion |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03407594_v194_n6_p523_Calvino http://hdl.handle.net/20.500.12110/paper_03407594_v194_n6_p523_Calvino |
| work_keys_str_mv |
AT calvinomariaana spatialspecificactionofserotoninwithintheleechmidbodyganglion AT szczupaklidia spatialspecificactionofserotoninwithintheleechmidbodyganglion |
| _version_ |
1768543704635670528 |