Effect of nitroxyl on human platelets function

There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The...

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Autores principales:	Saenz, Daniel Alberto, Rosenstein, Ruth Estela, Bari, Sara Elizabeth
Publicado:	2005
Materias:	Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v94_n3_p578_Bermejo http://hdl.handle.net/20.500.12110/paper_03406245_v94_n3_p578_Bermejo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03406245_v94_n3_p578_Bermejo
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spelling	paper:paper_03406245_v94_n3_p578_Bermejo2023-06-08T15:34:08Z Effect of nitroxyl on human platelets function Saenz, Daniel Alberto Rosenstein, Ruth Estela Bari, Sara Elizabeth Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present study was to comparatively analyze the effect of HNO and NO on several functional parameters of human platelets. For this purpose, sodium trioxodinitrate (Angeli's salt, AS) and sodium nitroprusside (SNP) were used as HNO and NO releasers, respectively. Both AS and SNP significantly inhibited platelet aggregation and ATP release induced by different agonists and adrenaline. AS or SNP did not modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV), whereas they substantially decreased the levels of CD62P, CD63 and of PAC-I (a platelet activated glycoprotein IIb/IIIa epitope) after the stimulation with ADP. AS and SNP significantly increased cGMP accumulation in a IH-[1,2,4]oxadiazolo [4,3-a] quinoxalin-I-one (ODQ)-sensitive manner. However while L-cysteine reduced the effect of AS, it increased the effect of SNP on this parameter. Accordingly, a differential effect of L-cysteine was observed on the antiaggregatory effect of both compounds. In summary, these results indicate that HNO is an effective inhibitor of human platelet aggregation. © 2005 Schattauer GmbH, Stuttgart. Fil:Sáenz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosenstein, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bari, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v94_n3_p578_Bermejo http://hdl.handle.net/20.500.12110/paper_03406245_v94_n3_p578_Bermejo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors
spellingShingle	Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors Saenz, Daniel Alberto Rosenstein, Ruth Estela Bari, Sara Elizabeth Effect of nitroxyl on human platelets function
topic_facet	Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors
description	There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present study was to comparatively analyze the effect of HNO and NO on several functional parameters of human platelets. For this purpose, sodium trioxodinitrate (Angeli's salt, AS) and sodium nitroprusside (SNP) were used as HNO and NO releasers, respectively. Both AS and SNP significantly inhibited platelet aggregation and ATP release induced by different agonists and adrenaline. AS or SNP did not modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV), whereas they substantially decreased the levels of CD62P, CD63 and of PAC-I (a platelet activated glycoprotein IIb/IIIa epitope) after the stimulation with ADP. AS and SNP significantly increased cGMP accumulation in a IH-[1,2,4]oxadiazolo [4,3-a] quinoxalin-I-one (ODQ)-sensitive manner. However while L-cysteine reduced the effect of AS, it increased the effect of SNP on this parameter. Accordingly, a differential effect of L-cysteine was observed on the antiaggregatory effect of both compounds. In summary, these results indicate that HNO is an effective inhibitor of human platelet aggregation. © 2005 Schattauer GmbH, Stuttgart.
author	Saenz, Daniel Alberto Rosenstein, Ruth Estela Bari, Sara Elizabeth
author_facet	Saenz, Daniel Alberto Rosenstein, Ruth Estela Bari, Sara Elizabeth
author_sort	Saenz, Daniel Alberto
title	Effect of nitroxyl on human platelets function
title_short	Effect of nitroxyl on human platelets function
title_full	Effect of nitroxyl on human platelets function
title_fullStr	Effect of nitroxyl on human platelets function
title_full_unstemmed	Effect of nitroxyl on human platelets function
title_sort	effect of nitroxyl on human platelets function
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v94_n3_p578_Bermejo http://hdl.handle.net/20.500.12110/paper_03406245_v94_n3_p578_Bermejo
work_keys_str_mv	AT saenzdanielalberto effectofnitroxylonhumanplateletsfunction AT rosensteinruthestela effectofnitroxylonhumanplateletsfunction AT barisaraelizabeth effectofnitroxylonhumanplateletsfunction
_version_	1768545738092969984

Effect of nitroxyl on human platelets function

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