Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes

Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of...

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Autores principales:	Talia, Paola M., Greizerstein, Eduardo José, Hopp, Horacio Esteban, Poggio, Lidia, Heinz, Ruth Amelia
Publicado:	2011
Materias:	BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia http://hdl.handle.net/20.500.12110/paper_03279545_v35_n1_p19_Talia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_03279545_v35_n1_p19_Talia2023-06-08T15:33:45Z Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes Talia, Paola M. Greizerstein, Eduardo José Hopp, Horacio Esteban Poggio, Lidia Heinz, Ruth Amelia BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect CPRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower. Fil:Talia, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Greizerstein, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia http://hdl.handle.net/20.500.12110/paper_03279545_v35_n1_p19_Talia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA
spellingShingle	BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA Talia, Paola M. Greizerstein, Eduardo José Hopp, Horacio Esteban Poggio, Lidia Heinz, Ruth Amelia Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
topic_facet	BAC-FISH C-PRINS Physical mapping Single-copy sequences Sunflower plant DNA article bacterial artificial chromosome fluorescence in situ hybridization chromosome identification controlled study cycling primed in situ labeling fluorescence in situ hybridization gene mapping genetic linkage genetic marker nonhuman nucleotide repeat nucleotide sequence plant chromosome primed in situ labeling process optimization quantitative trait locus Sclerotinia sclerotiorum sequence analysis signal detection simple sequence repeat sunflower bacterial artificial chromosome DNA sequence genetics methodology Bacteria (microorganisms) Helianthus Helianthus annuus Base Sequence Chromosomes, Artificial, Bacterial Chromosomes, Plant Genetic Markers Helianthus In Situ Hybridization, Fluorescence Quantitative Trait Loci Sequence Analysis, DNA
description	Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect CPRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower.
author	Talia, Paola M. Greizerstein, Eduardo José Hopp, Horacio Esteban Poggio, Lidia Heinz, Ruth Amelia
author_facet	Talia, Paola M. Greizerstein, Eduardo José Hopp, Horacio Esteban Poggio, Lidia Heinz, Ruth Amelia
author_sort	Talia, Paola M.
title	Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
title_short	Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
title_full	Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
title_fullStr	Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
title_full_unstemmed	Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
title_sort	detection of single copy sequences using bac-fish and c-prins techniques in sunflower chromosomes
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia http://hdl.handle.net/20.500.12110/paper_03279545_v35_n1_p19_Talia
work_keys_str_mv	AT taliapaolam detectionofsinglecopysequencesusingbacfishandcprinstechniquesinsunflowerchromosomes AT greizersteineduardojose detectionofsinglecopysequencesusingbacfishandcprinstechniquesinsunflowerchromosomes AT hopphoracioesteban detectionofsinglecopysequencesusingbacfishandcprinstechniquesinsunflowerchromosomes AT poggiolidia detectionofsinglecopysequencesusingbacfishandcprinstechniquesinsunflowerchromosomes AT heinzruthamelia detectionofsinglecopysequencesusingbacfishandcprinstechniquesinsunflowerchromosomes
_version_	1768546116819746816

Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes

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