Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos |
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paper:paper_03279545_v20_n3_p265_AldanaMarcos2023-06-08T15:33:36Z Standardization of fixation, processing and staining methods for the central nervous system of vertebrates Aldana Marcos, Hernán Javier Ferrari, Carina Cintia Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories. Fil:Aldana Marcos, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrari, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates |
| spellingShingle |
Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates Aldana Marcos, Hernán Javier Ferrari, Carina Cintia Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| topic_facet |
Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates |
| description |
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories. |
| author |
Aldana Marcos, Hernán Javier Ferrari, Carina Cintia |
| author_facet |
Aldana Marcos, Hernán Javier Ferrari, Carina Cintia |
| author_sort |
Aldana Marcos, Hernán Javier |
| title |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_short |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_full |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_fullStr |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_full_unstemmed |
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| title_sort |
standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos |
| work_keys_str_mv |
AT aldanamarcoshernanjavier standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates AT ferraricarinacintia standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates |
| _version_ |
1768542315343773696 |