Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding

Bacillus anthracis protective antigen (PA) is a well known and relevant immunogenic protein that is the basis for both anthrax vaccines and diagnostic methods. Properly folded antigenic PA is necessary for these applications. In this study a high level of PA was obtained in recombinant Escherichia c...

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Guardado en:
Detalles Bibliográficos
Publicado: 2016
Materias:
Bacillus anthracis
High-throughput refolding screening
Protective antigen
Protein refolding
bacterial antigen
antigen expression
Article
dot hybridization
high throughput screening
molecular cloning
nonhuman
polymerase chain reaction
protein expression
protein purification
protein refolding
biosynthesis
chemical structure
immunology
metabolism
Antigens, Bacterial
Models, Molecular
Protein Refolding
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v48_n1_p5_Pavan
http://hdl.handle.net/20.500.12110/paper_03257541_v48_n1_p5_Pavan
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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