Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum act...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v42_n1_p57_Salariato http://hdl.handle.net/20.500.12110/paper_03257541_v42_n1_p57_Salariato |
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paper:paper_03257541_v42_n1_p57_Salariato2023-06-08T15:32:53Z Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v42_n1_p57_Salariato http://hdl.handle.net/20.500.12110/paper_03257541_v42_n1_p57_Salariato |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature |
| spellingShingle |
Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| topic_facet |
Fomes sclerodermeus Polygalacturonase Solid state fermentation polygalacturonase sodium sulfate article enzyme synthesis Fomes sclerodermeus fungus growth nonhuman solid state fermentation Cell Fractionation Coriolaceae Fermentation Fungal Proteins Hydrogen-Ion Concentration Mycology Polygalacturonase Solvents Temperature |
| description |
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained. |
| title |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_short |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_full |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_fullStr |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_full_unstemmed |
Extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| title_sort |
extraction and characterization of polygalacturonase of fomes sclerodermeus produced by solid-state fermentation |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v42_n1_p57_Salariato http://hdl.handle.net/20.500.12110/paper_03257541_v42_n1_p57_Salariato |
| _version_ |
1768542270079893504 |