Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition

A mathematical approach was applied in order to optimize the effect of electroporation by application of pulsed electric fields (PEF) and nisin addition on the inhibition of a strain of Pseudomonas aeruginosa isolated from river sediments. This strain showed to be highly resistant to nisin as only t...

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Autores principales:	Cerrutti, Patricia, Pilosof, Ana María Renata
Publicado:	2003
Materias:	Electroporation Mathematical model Nisin Pseudomonas aeruginosa bacteriocin nisin article bacterial growth bacterium isolate electric field electroporation growth inhibition mathematical analysis mathematical model nonhuman river ecosystem sediment Dose-Response Relationship, Drug Electromagnetic Fields Electrophoresis, Gel, Pulsed-Field Microbial Sensitivity Tests Models, Theoretical Regression Analysis Water Microbiology
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi http://hdl.handle.net/20.500.12110/paper_03257541_v35_n4_p198_Santi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03257541_v35_n4_p198_Santi
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spelling	paper:paper_03257541_v35_n4_p198_Santi2023-06-08T15:32:51Z Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition Cerrutti, Patricia Pilosof, Ana María Renata Electroporation Mathematical model Nisin Pseudomonas aeruginosa bacteriocin nisin article bacterial growth bacterium isolate electric field electroporation growth inhibition mathematical analysis mathematical model nonhuman Pseudomonas aeruginosa river ecosystem sediment Dose-Response Relationship, Drug Electromagnetic Fields Electrophoresis, Gel, Pulsed-Field Electroporation Microbial Sensitivity Tests Models, Theoretical Nisin Pseudomonas aeruginosa Regression Analysis Water Microbiology A mathematical approach was applied in order to optimize the effect of electroporation by application of pulsed electric fields (PEF) and nisin addition on the inhibition of a strain of Pseudomonas aeruginosa isolated from river sediments. This strain showed to be highly resistant to nisin as only two log cycles reduction of viable cells were obtained in the presence of 84,000 IU/ml nisin. But when a combination of bacteriocin and selected PEF treatment conditions were applied, 4.4-decimal log cycle reduction could be achieved. PEF and nisin interaction seems to be complex, as at lower electric field intensities (i.e., 5 kV/cm) an increment in the number of pulses applied clearly induced a lower inhibitory effect of nisin. At higher PEF intensities (i.e., 11 kV/cm), the inhibitory effect of nisin increased with the number of pulses applied. Results overall, the obtained indicate the possibility of combining PEF and nisin treatments in order to improve the inhibition of resistant microorganisms. The Doehlert experimental design and surface response methodology was an interesting tool to obtain or predict the optimal combination of the stress factors applied. Fil:Cerrutti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi http://hdl.handle.net/20.500.12110/paper_03257541_v35_n4_p198_Santi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Electroporation Mathematical model Nisin Pseudomonas aeruginosa bacteriocin nisin article bacterial growth bacterium isolate electric field electroporation growth inhibition mathematical analysis mathematical model nonhuman Pseudomonas aeruginosa river ecosystem sediment Dose-Response Relationship, Drug Electromagnetic Fields Electrophoresis, Gel, Pulsed-Field Electroporation Microbial Sensitivity Tests Models, Theoretical Nisin Pseudomonas aeruginosa Regression Analysis Water Microbiology
spellingShingle	Electroporation Mathematical model Nisin Pseudomonas aeruginosa bacteriocin nisin article bacterial growth bacterium isolate electric field electroporation growth inhibition mathematical analysis mathematical model nonhuman Pseudomonas aeruginosa river ecosystem sediment Dose-Response Relationship, Drug Electromagnetic Fields Electrophoresis, Gel, Pulsed-Field Electroporation Microbial Sensitivity Tests Models, Theoretical Nisin Pseudomonas aeruginosa Regression Analysis Water Microbiology Cerrutti, Patricia Pilosof, Ana María Renata Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition
topic_facet	Electroporation Mathematical model Nisin Pseudomonas aeruginosa bacteriocin nisin article bacterial growth bacterium isolate electric field electroporation growth inhibition mathematical analysis mathematical model nonhuman Pseudomonas aeruginosa river ecosystem sediment Dose-Response Relationship, Drug Electromagnetic Fields Electrophoresis, Gel, Pulsed-Field Electroporation Microbial Sensitivity Tests Models, Theoretical Nisin Pseudomonas aeruginosa Regression Analysis Water Microbiology
description	A mathematical approach was applied in order to optimize the effect of electroporation by application of pulsed electric fields (PEF) and nisin addition on the inhibition of a strain of Pseudomonas aeruginosa isolated from river sediments. This strain showed to be highly resistant to nisin as only two log cycles reduction of viable cells were obtained in the presence of 84,000 IU/ml nisin. But when a combination of bacteriocin and selected PEF treatment conditions were applied, 4.4-decimal log cycle reduction could be achieved. PEF and nisin interaction seems to be complex, as at lower electric field intensities (i.e., 5 kV/cm) an increment in the number of pulses applied clearly induced a lower inhibitory effect of nisin. At higher PEF intensities (i.e., 11 kV/cm), the inhibitory effect of nisin increased with the number of pulses applied. Results overall, the obtained indicate the possibility of combining PEF and nisin treatments in order to improve the inhibition of resistant microorganisms. The Doehlert experimental design and surface response methodology was an interesting tool to obtain or predict the optimal combination of the stress factors applied.
author	Cerrutti, Patricia Pilosof, Ana María Renata
author_facet	Cerrutti, Patricia Pilosof, Ana María Renata
author_sort	Cerrutti, Patricia
title	Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition
title_short	Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition
title_full	Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition
title_fullStr	Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition
title_full_unstemmed	Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition
title_sort	optimization of electroporation conditions along with nisin treatment for pseudomonas aeruginosa inhibition
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi http://hdl.handle.net/20.500.12110/paper_03257541_v35_n4_p198_Santi
work_keys_str_mv	AT cerruttipatricia optimizationofelectroporationconditionsalongwithnisintreatmentforpseudomonasaeruginosainhibition AT pilosofanamariarenata optimizationofelectroporationconditionsalongwithnisintreatmentforpseudomonasaeruginosainhibition
_version_	1768545880396267520

Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition

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