Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)

Growth and cellulase-xylanase enzyme systems production by Ascobolus gamundii were evaluated in synthetic media with or without natural inducers. The basal level of enzyme complexes was determined using glucose as carbon source. With crystalline cellulose, the amount of endoglucanase and beta-glucos...

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Autores principales:	Sivori, Andrea Silvia, Mercuri, Oscar Alberto, Forchíassin, Flavia
Publicado:	1996
Materias:	beta glucosidase cellulase cellulose endo 1,4 beta xylanase exo 1,4 beta D xylosidase exo-1,4-beta-D-xylosidase fungal protein glucose xylan xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article Ascomycetes biosynthesis enzyme induction enzymology feces gene expression regulation genetics kinetics metabolism microbiology Ascomycota beta-Glucosidase Cellulase Cellulose Endo-1,4-beta Xylanases Enzyme Induction Feces Fungal Proteins Gene Expression Regulation, Bacterial Glucose Kinetics Xylan Endo-1,3-beta-Xylosidase Xylans Xylosidases
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n1_p9_Sivori http://hdl.handle.net/20.500.12110/paper_03257541_v28_n1_p9_Sivori
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03257541_v28_n1_p9_Sivori
record_format	dspace
spelling	paper:paper_03257541_v28_n1_p9_Sivori2023-06-08T15:32:39Z Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina) Sivori, Andrea Silvia Mercuri, Oscar Alberto Forchíassin, Flavia beta glucosidase cellulase cellulose endo 1,4 beta xylanase exo 1,4 beta D xylosidase exo-1,4-beta-D-xylosidase fungal protein glucose xylan xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article Ascomycetes biosynthesis enzyme induction enzymology feces gene expression regulation genetics kinetics metabolism microbiology Ascomycota beta-Glucosidase Cellulase Cellulose Endo-1,4-beta Xylanases Enzyme Induction Feces Fungal Proteins Gene Expression Regulation, Bacterial Glucose Kinetics Xylan Endo-1,3-beta-Xylosidase Xylans Xylosidases Growth and cellulase-xylanase enzyme systems production by Ascobolus gamundii were evaluated in synthetic media with or without natural inducers. The basal level of enzyme complexes was determined using glucose as carbon source. With crystalline cellulose, the amount of endoglucanase and beta-glucosidase was 2-4 times the basal, and the level of endoxylanase and beta-xylosidase was 3 times higher than that observed with glucose. When using xylan as carbon source, endoxylanase and endoglucanase were induced. The higher production of the enzyme systems was observed during the log-phase growth of A. gamundii with a mixture of cellulose and xylan as carbon source. The generalized induction of both enzyme complexes could be due to evolutive adaptation to the substrate (herbivore dung) in which cellulose and hemicellulose are associated to conform the plants cell-walls. Fil:Sivori, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mercuri, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n1_p9_Sivori http://hdl.handle.net/20.500.12110/paper_03257541_v28_n1_p9_Sivori
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	beta glucosidase cellulase cellulose endo 1,4 beta xylanase exo 1,4 beta D xylosidase exo-1,4-beta-D-xylosidase fungal protein glucose xylan xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article Ascomycetes biosynthesis enzyme induction enzymology feces gene expression regulation genetics kinetics metabolism microbiology Ascomycota beta-Glucosidase Cellulase Cellulose Endo-1,4-beta Xylanases Enzyme Induction Feces Fungal Proteins Gene Expression Regulation, Bacterial Glucose Kinetics Xylan Endo-1,3-beta-Xylosidase Xylans Xylosidases
spellingShingle	beta glucosidase cellulase cellulose endo 1,4 beta xylanase exo 1,4 beta D xylosidase exo-1,4-beta-D-xylosidase fungal protein glucose xylan xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article Ascomycetes biosynthesis enzyme induction enzymology feces gene expression regulation genetics kinetics metabolism microbiology Ascomycota beta-Glucosidase Cellulase Cellulose Endo-1,4-beta Xylanases Enzyme Induction Feces Fungal Proteins Gene Expression Regulation, Bacterial Glucose Kinetics Xylan Endo-1,3-beta-Xylosidase Xylans Xylosidases Sivori, Andrea Silvia Mercuri, Oscar Alberto Forchíassin, Flavia Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)
topic_facet	beta glucosidase cellulase cellulose endo 1,4 beta xylanase exo 1,4 beta D xylosidase exo-1,4-beta-D-xylosidase fungal protein glucose xylan xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article Ascomycetes biosynthesis enzyme induction enzymology feces gene expression regulation genetics kinetics metabolism microbiology Ascomycota beta-Glucosidase Cellulase Cellulose Endo-1,4-beta Xylanases Enzyme Induction Feces Fungal Proteins Gene Expression Regulation, Bacterial Glucose Kinetics Xylan Endo-1,3-beta-Xylosidase Xylans Xylosidases
description	Growth and cellulase-xylanase enzyme systems production by Ascobolus gamundii were evaluated in synthetic media with or without natural inducers. The basal level of enzyme complexes was determined using glucose as carbon source. With crystalline cellulose, the amount of endoglucanase and beta-glucosidase was 2-4 times the basal, and the level of endoxylanase and beta-xylosidase was 3 times higher than that observed with glucose. When using xylan as carbon source, endoxylanase and endoglucanase were induced. The higher production of the enzyme systems was observed during the log-phase growth of A. gamundii with a mixture of cellulose and xylan as carbon source. The generalized induction of both enzyme complexes could be due to evolutive adaptation to the substrate (herbivore dung) in which cellulose and hemicellulose are associated to conform the plants cell-walls.
author	Sivori, Andrea Silvia Mercuri, Oscar Alberto Forchíassin, Flavia
author_facet	Sivori, Andrea Silvia Mercuri, Oscar Alberto Forchíassin, Flavia
author_sort	Sivori, Andrea Silvia
title	Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)
title_short	Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)
title_full	Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)
title_fullStr	Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)
title_full_unstemmed	Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)
title_sort	kinetics of xylanase and cellulase production by ascobolus gamundii (fungi, ascomycotina)
publishDate	1996
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n1_p9_Sivori http://hdl.handle.net/20.500.12110/paper_03257541_v28_n1_p9_Sivori
work_keys_str_mv	AT sivoriandreasilvia kineticsofxylanaseandcellulaseproductionbyascobolusgamundiifungiascomycotina AT mercurioscaralberto kineticsofxylanaseandcellulaseproductionbyascobolusgamundiifungiascomycotina AT forchiassinflavia kineticsofxylanaseandcellulaseproductionbyascobolusgamundiifungiascomycotina
_version_	1768544226341027840

Kinetics of xylanase and cellulase production by Ascobolus gamundii (Fungi, Ascomycotina)

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