Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis

Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Alconada Magliano, Teresa María
Publicado:	1995
Materias:	cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n4_p191_Alconada http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03257541_v27_n4_p191_Alconada
record_format	dspace
spelling	paper:paper_03257541_v27_n4_p191_Alconada2023-06-08T15:32:39Z Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis Alconada Magliano, Teresa María cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. Protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. This fact suggest a possible relationship between the enzymes produced by Fusarium oxysporum f.sp. melonis and their pathogenicity on melon plants. Fil:Alconada, T.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n4_p191_Alconada http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases
spellingShingle	cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases Alconada Magliano, Teresa María Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
topic_facet	cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases
description	Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. Protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. This fact suggest a possible relationship between the enzymes produced by Fusarium oxysporum f.sp. melonis and their pathogenicity on melon plants.
author	Alconada Magliano, Teresa María
author_facet	Alconada Magliano, Teresa María
author_sort	Alconada Magliano, Teresa María
title	Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
title_short	Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
title_full	Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
title_fullStr	Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
title_full_unstemmed	Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
title_sort	isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
publishDate	1995
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n4_p191_Alconada http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada
work_keys_str_mv	AT alconadamaglianoteresamaria isolationofprotoplastsfromvegetabletissuesusingextracellularlyticenzymesfromfusariumoxysporumfspmelonis
_version_	1768543370308747264

Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis

Ejemplares similares