Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)

Meliacine, a peptide associated with antiviral activity isolated from the high plant Melia azedarach L (MA) inhibits the replication of different strains of foot and mouth disease virus (FMDV) and poliovirus in BHK-21 or Vero cells, respectively, infected at a multiplicity of infection (m.o.i.) of 1...

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Detalles Bibliográficos
Autores principales: Wachsman, Mónica Beatriz, Coto, Celia Esther
Publicado: 1995
Materias:
antivirus agent
meliacin
peptide
plant extract
vegetable protein
article
drug effect
microbiological examination
Picornavirus
Antiviral Agents
Microbial Sensitivity Tests
Peptides
Picornaviridae
Plant Extracts
Plant Proteins
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n1_p33_Wachsman
http://hdl.handle.net/20.500.12110/paper_03257541_v27_n1_p33_Wachsman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03257541_v27_n1_p33_Wachsman
record_format dspace
spelling paper:paper_03257541_v27_n1_p33_Wachsman2023-06-08T15:32:38Z Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin) Wachsman, Mónica Beatriz Coto, Celia Esther antivirus agent meliacin peptide plant extract vegetable protein article drug effect microbiological examination Picornavirus Antiviral Agents Microbial Sensitivity Tests Peptides Picornaviridae Plant Extracts Plant Proteins Meliacine, a peptide associated with antiviral activity isolated from the high plant Melia azedarach L (MA) inhibits the replication of different strains of foot and mouth disease virus (FMDV) and poliovirus in BHK-21 or Vero cells, respectively, infected at a multiplicity of infection (m.o.i.) of 1. A leaf extract of MA, containing meliacine was added to the culture medium after virus adsorption and maintained up to virus harvest (18 hs). FMDV O1 Campos and O69 strains showed 60% and 52% inhibition, respectively. A24 and A87 strains proved to be inhibited more than 90%, and the C3 Resende and O1 Case-ros strains were not sensitive to the extract. Regarding the three types of poliovirus, the most susceptible one was type 1 (84%). Types 2 and 3 were inhibited only 69% and 32%, respectively. Treatment of the cells with the antiviral for 24 hours before infection (pretreatment), was not effective for the two above mentioned viruses. Since the extract of MA is very active against other RNA viruses (i.e. vesicular stomatitis virus), the susceptibility of FMDV strain O1 Campos and poliovirus type 3 was tested using lower m.o.i. The results showed that MA produced an inhibition of viral replication of 99% at a m.o.i. of 0.001 in both cases. These results suggest that picornaviruses are also susceptible to MA. However, to demonstrate its antiviral effect it is necessary to slow down the rate of virus-cell killing by reducing the m.o.i. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n1_p33_Wachsman http://hdl.handle.net/20.500.12110/paper_03257541_v27_n1_p33_Wachsman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic antivirus agent
meliacin
peptide
plant extract
vegetable protein
article
drug effect
microbiological examination
Picornavirus
Antiviral Agents
Microbial Sensitivity Tests
Peptides
Picornaviridae
Plant Extracts
Plant Proteins
spellingShingle antivirus agent
meliacin
peptide
plant extract
vegetable protein
article
drug effect
microbiological examination
Picornavirus
Antiviral Agents
Microbial Sensitivity Tests
Peptides
Picornaviridae
Plant Extracts
Plant Proteins
Wachsman, Mónica Beatriz
Coto, Celia Esther
Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
topic_facet antivirus agent
meliacin
peptide
plant extract
vegetable protein
article
drug effect
microbiological examination
Picornavirus
Antiviral Agents
Microbial Sensitivity Tests
Peptides
Picornaviridae
Plant Extracts
Plant Proteins
description Meliacine, a peptide associated with antiviral activity isolated from the high plant Melia azedarach L (MA) inhibits the replication of different strains of foot and mouth disease virus (FMDV) and poliovirus in BHK-21 or Vero cells, respectively, infected at a multiplicity of infection (m.o.i.) of 1. A leaf extract of MA, containing meliacine was added to the culture medium after virus adsorption and maintained up to virus harvest (18 hs). FMDV O1 Campos and O69 strains showed 60% and 52% inhibition, respectively. A24 and A87 strains proved to be inhibited more than 90%, and the C3 Resende and O1 Case-ros strains were not sensitive to the extract. Regarding the three types of poliovirus, the most susceptible one was type 1 (84%). Types 2 and 3 were inhibited only 69% and 32%, respectively. Treatment of the cells with the antiviral for 24 hours before infection (pretreatment), was not effective for the two above mentioned viruses. Since the extract of MA is very active against other RNA viruses (i.e. vesicular stomatitis virus), the susceptibility of FMDV strain O1 Campos and poliovirus type 3 was tested using lower m.o.i. The results showed that MA produced an inhibition of viral replication of 99% at a m.o.i. of 0.001 in both cases. These results suggest that picornaviruses are also susceptible to MA. However, to demonstrate its antiviral effect it is necessary to slow down the rate of virus-cell killing by reducing the m.o.i.
author Wachsman, Mónica Beatriz
Coto, Celia Esther
author_facet Wachsman, Mónica Beatriz
Coto, Celia Esther
author_sort Wachsman, Mónica Beatriz
title Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
title_short Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
title_full Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
title_fullStr Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
title_full_unstemmed Susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
title_sort susceptibility of picornaviruses++ to an antiviral of plant origin (meliacin)
publishDate 1995
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n1_p33_Wachsman
http://hdl.handle.net/20.500.12110/paper_03257541_v27_n1_p33_Wachsman
work_keys_str_mv AT wachsmanmonicabeatriz susceptibilityofpicornavirusestoanantiviralofplantoriginmeliacin
AT cotoceliaesther susceptibilityofpicornavirusestoanantiviralofplantoriginmeliacin
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