Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine sho...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pujol, Carlos Alberto, Massouh, Ernesto Jorge
Publicado:	1992
Materias:	virus antibody virus vaccine animal Aphthovirus article bioassay biosynthesis blood comparative study enzyme linked immunosorbent assay evaluation foot and mouth disease hemagglutination test immunology male mouse mouse strain serodiagnosis suckling vaccination Animals Animals, Suckling Antibodies, Viral Biological Assay Enzyme-Linked Immunosorbent Assay Evaluation Studies Foot-and-Mouth Disease Hemagglutination Tests Male Mice Mice, Inbred Strains Neutralization Tests Vaccination Viral Vaccines
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v24_n3-4_p126_Feledi http://hdl.handle.net/20.500.12110/paper_03257541_v24_n3-4_p126_Feledi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03257541_v24_n3-4_p126_Feledi
record_format	dspace
spelling	paper:paper_03257541_v24_n3-4_p126_Feledi2025-07-30T18:09:58Z Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus Pujol, Carlos Alberto Massouh, Ernesto Jorge virus antibody virus vaccine animal Aphthovirus article bioassay biosynthesis blood comparative study enzyme linked immunosorbent assay evaluation foot and mouth disease hemagglutination test immunology male mouse mouse strain serodiagnosis suckling vaccination Animals Animals, Suckling Antibodies, Viral Aphthovirus Biological Assay Enzyme-Linked Immunosorbent Assay Evaluation Studies Foot-and-Mouth Disease Hemagglutination Tests Male Mice Mice, Inbred Strains Neutralization Tests Vaccination Viral Vaccines Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Massouh, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v24_n3-4_p126_Feledi http://hdl.handle.net/20.500.12110/paper_03257541_v24_n3-4_p126_Feledi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	virus antibody virus vaccine animal Aphthovirus article bioassay biosynthesis blood comparative study enzyme linked immunosorbent assay evaluation foot and mouth disease hemagglutination test immunology male mouse mouse strain serodiagnosis suckling vaccination Animals Animals, Suckling Antibodies, Viral Aphthovirus Biological Assay Enzyme-Linked Immunosorbent Assay Evaluation Studies Foot-and-Mouth Disease Hemagglutination Tests Male Mice Mice, Inbred Strains Neutralization Tests Vaccination Viral Vaccines
spellingShingle	virus antibody virus vaccine animal Aphthovirus article bioassay biosynthesis blood comparative study enzyme linked immunosorbent assay evaluation foot and mouth disease hemagglutination test immunology male mouse mouse strain serodiagnosis suckling vaccination Animals Animals, Suckling Antibodies, Viral Aphthovirus Biological Assay Enzyme-Linked Immunosorbent Assay Evaluation Studies Foot-and-Mouth Disease Hemagglutination Tests Male Mice Mice, Inbred Strains Neutralization Tests Vaccination Viral Vaccines Pujol, Carlos Alberto Massouh, Ernesto Jorge Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
topic_facet	virus antibody virus vaccine animal Aphthovirus article bioassay biosynthesis blood comparative study enzyme linked immunosorbent assay evaluation foot and mouth disease hemagglutination test immunology male mouse mouse strain serodiagnosis suckling vaccination Animals Animals, Suckling Antibodies, Viral Aphthovirus Biological Assay Enzyme-Linked Immunosorbent Assay Evaluation Studies Foot-and-Mouth Disease Hemagglutination Tests Male Mice Mice, Inbred Strains Neutralization Tests Vaccination Viral Vaccines
description	Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.
author	Pujol, Carlos Alberto Massouh, Ernesto Jorge
author_facet	Pujol, Carlos Alberto Massouh, Ernesto Jorge
author_sort	Pujol, Carlos Alberto
title	Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
title_short	Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
title_full	Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
title_fullStr	Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
title_full_unstemmed	Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
title_sort	comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus
publishDate	1992
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v24_n3-4_p126_Feledi http://hdl.handle.net/20.500.12110/paper_03257541_v24_n3-4_p126_Feledi
work_keys_str_mv	AT pujolcarlosalberto comparisonofvariousmethodsforthemeasurementofthehumoralimmuneresponseinmicevaccinatedwithaphthousfevervirus AT massouhernestojorge comparisonofvariousmethodsforthemeasurementofthehumoralimmuneresponseinmicevaccinatedwithaphthousfevervirus
_version_	1840320771919970304

Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus

Ejemplares similares