Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados

Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosacc...

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Autores principales: Tortorella, Hélen, Brandelli, Adriano, Mazzini, María Nélida
Publicado: 1996
Materias:
Affinity chromatography
Carbohydrate
Glycoprotein
Lectin
Semen
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella
http://hdl.handle.net/20.500.12110/paper_03252957_v30_n1_p49_Tortorella
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03252957_v30_n1_p49_Tortorella
record_format dspace
spelling paper:paper_03252957_v30_n1_p49_Tortorella2023-06-08T15:32:14Z Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados Tortorella, Hélen Brandelli, Adriano Mazzini, María Nélida Affinity chromatography Carbohydrate Glycoprotein Lectin Semen Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosaccharide chains of F1 and F2 showed that galactose is always the main component together with fucose and variable amounts of mannose, N-acetylglucosamine and N-acetylgalactosamine. These fractions showed differences in the primary structure of glycoprotein glycans (N- and O-glycosidic-linked), according to the pathology. All the oligosaccharide chains in F1 correspond to mucin type glycoproteins (O-glycosidic type), whereas in F2 the molar ratio O-/N- is 5.3/1. F1 from azoospermic and oligozoospermic are nearly all of the O-glycosid type while in vasectomised the oligosaccharide chains differ from the other ones by a molar ratio O-/N- of 1.7/1. F2 present both O- and N- oligosaccharide chains in different amounts, with the only exception of vasectomised which belongs to O-glycosid type. The results obtained suggest that the composition of major PSH fractions from donors with different pathologies is characterized by marked structural differences on the glycan and the protein moiety. Fil:Tortorella, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Brandelli, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzini, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella http://hdl.handle.net/20.500.12110/paper_03252957_v30_n1_p49_Tortorella
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Affinity chromatography
Carbohydrate
Glycoprotein
Lectin
Semen
spellingShingle Affinity chromatography
Carbohydrate
Glycoprotein
Lectin
Semen
Tortorella, Hélen
Brandelli, Adriano
Mazzini, María Nélida
Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
topic_facet Affinity chromatography
Carbohydrate
Glycoprotein
Lectin
Semen
description Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosaccharide chains of F1 and F2 showed that galactose is always the main component together with fucose and variable amounts of mannose, N-acetylglucosamine and N-acetylgalactosamine. These fractions showed differences in the primary structure of glycoprotein glycans (N- and O-glycosidic-linked), according to the pathology. All the oligosaccharide chains in F1 correspond to mucin type glycoproteins (O-glycosidic type), whereas in F2 the molar ratio O-/N- is 5.3/1. F1 from azoospermic and oligozoospermic are nearly all of the O-glycosid type while in vasectomised the oligosaccharide chains differ from the other ones by a molar ratio O-/N- of 1.7/1. F2 present both O- and N- oligosaccharide chains in different amounts, with the only exception of vasectomised which belongs to O-glycosid type. The results obtained suggest that the composition of major PSH fractions from donors with different pathologies is characterized by marked structural differences on the glycan and the protein moiety.
author Tortorella, Hélen
Brandelli, Adriano
Mazzini, María Nélida
author_facet Tortorella, Hélen
Brandelli, Adriano
Mazzini, María Nélida
author_sort Tortorella, Hélen
title Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_short Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_full Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_fullStr Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_full_unstemmed Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
title_sort caracterización de fracciones mayoritarias del plasma seminal humano. estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
publishDate 1996
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella
http://hdl.handle.net/20.500.12110/paper_03252957_v30_n1_p49_Tortorella
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