Protein deterioration and longevity of quinoa seeds during long-term storage
Seed protein stability and seed deterioration during storage were studied in seeds of two different quinoa (Chenopodium quinoa Willd.) cultivars, i.e., cv. Ollagüe and cv. Baer II. Germination and viability tests proved that cv. Baer II was more longevous than cv. Ollagüe. Protein insolubilisation w...
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2010
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paper:paper_03088146_v121_n4_p952_Castellion2023-06-08T15:31:41Z Protein deterioration and longevity of quinoa seeds during long-term storage Castellión, Martina Laura Matiacevich, Silvia Beatriz Buera, María del Pilar Chenopodium quinoa Maillard Protein solubility Seed ageing Seed conservation Seed storage proteins advanced glycation end product article Chenopodium quinoa conservational seed bank controlled study deterioration germination glycation nonhuman plant seed protein solubility protein stability protein structure, function and variability seed storage Chenopodium quinoa Seed protein stability and seed deterioration during storage were studied in seeds of two different quinoa (Chenopodium quinoa Willd.) cultivars, i.e., cv. Ollagüe and cv. Baer II. Germination and viability tests proved that cv. Baer II was more longevous than cv. Ollagüe. Protein insolubilisation was detected during storage and correlated with longevity. However, protein solubility was restored by priming in both cultivars, disregarding their germination capability. Extremely high contents of advanced glycation end-products (AGEs), assessed by carboxymethyllysine (CML) quantification, were obtained from stored seeds as viability was reduced; primed seeds showed slightly lower AGE content, indicating a strong association between quinoa seed ageing and Maillard products accumulation. High intensity fluorescence values were observed in seeds stored in the gene bank bearing 100% germination values, which indicate that the detection of fluorescence is not by itself an appropriate indicator of protein damage by Maillard-type product accumulation in quinoa seeds. This work establishes for the first time a direct association between seed viability and AGE accumulation, due to Maillard reaction, in quinoa seeds. © 2010 Elsevier Ltd. All rights reserved. Fil:Castellión, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matiacevich, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buera, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03088146_v121_n4_p952_Castellion http://hdl.handle.net/20.500.12110/paper_03088146_v121_n4_p952_Castellion |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chenopodium quinoa Maillard Protein solubility Seed ageing Seed conservation Seed storage proteins advanced glycation end product article Chenopodium quinoa conservational seed bank controlled study deterioration germination glycation nonhuman plant seed protein solubility protein stability protein structure, function and variability seed storage Chenopodium quinoa |
| spellingShingle |
Chenopodium quinoa Maillard Protein solubility Seed ageing Seed conservation Seed storage proteins advanced glycation end product article Chenopodium quinoa conservational seed bank controlled study deterioration germination glycation nonhuman plant seed protein solubility protein stability protein structure, function and variability seed storage Chenopodium quinoa Castellión, Martina Laura Matiacevich, Silvia Beatriz Buera, María del Pilar Protein deterioration and longevity of quinoa seeds during long-term storage |
| topic_facet |
Chenopodium quinoa Maillard Protein solubility Seed ageing Seed conservation Seed storage proteins advanced glycation end product article Chenopodium quinoa conservational seed bank controlled study deterioration germination glycation nonhuman plant seed protein solubility protein stability protein structure, function and variability seed storage Chenopodium quinoa |
| description |
Seed protein stability and seed deterioration during storage were studied in seeds of two different quinoa (Chenopodium quinoa Willd.) cultivars, i.e., cv. Ollagüe and cv. Baer II. Germination and viability tests proved that cv. Baer II was more longevous than cv. Ollagüe. Protein insolubilisation was detected during storage and correlated with longevity. However, protein solubility was restored by priming in both cultivars, disregarding their germination capability. Extremely high contents of advanced glycation end-products (AGEs), assessed by carboxymethyllysine (CML) quantification, were obtained from stored seeds as viability was reduced; primed seeds showed slightly lower AGE content, indicating a strong association between quinoa seed ageing and Maillard products accumulation. High intensity fluorescence values were observed in seeds stored in the gene bank bearing 100% germination values, which indicate that the detection of fluorescence is not by itself an appropriate indicator of protein damage by Maillard-type product accumulation in quinoa seeds. This work establishes for the first time a direct association between seed viability and AGE accumulation, due to Maillard reaction, in quinoa seeds. © 2010 Elsevier Ltd. All rights reserved. |
| author |
Castellión, Martina Laura Matiacevich, Silvia Beatriz Buera, María del Pilar |
| author_facet |
Castellión, Martina Laura Matiacevich, Silvia Beatriz Buera, María del Pilar |
| author_sort |
Castellión, Martina Laura |
| title |
Protein deterioration and longevity of quinoa seeds during long-term storage |
| title_short |
Protein deterioration and longevity of quinoa seeds during long-term storage |
| title_full |
Protein deterioration and longevity of quinoa seeds during long-term storage |
| title_fullStr |
Protein deterioration and longevity of quinoa seeds during long-term storage |
| title_full_unstemmed |
Protein deterioration and longevity of quinoa seeds during long-term storage |
| title_sort |
protein deterioration and longevity of quinoa seeds during long-term storage |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03088146_v121_n4_p952_Castellion http://hdl.handle.net/20.500.12110/paper_03088146_v121_n4_p952_Castellion |
| work_keys_str_mv |
AT castellionmartinalaura proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage AT matiacevichsilviabeatriz proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage AT bueramariadelpilar proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage |
| _version_ |
1768543703480139776 |