The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus
Evidence for the participation of Rel/NF-κB transcription factors in long-term memory has recently been reported in the context-signal learning paradigm of the crab Chasmagnathus, in which a high correlation between long-term memory formation and NF-κB activation was observed. Two components of the...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v112_n1_p161_Merlo http://hdl.handle.net/20.500.12110/paper_03064522_v112_n1_p161_Merlo |
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paper:paper_03064522_v112_n1_p161_Merlo2023-06-08T15:31:12Z The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus Crab IκB kinase long-term memory Neural plasticity Rel/NF-κB I kappa B kinase immunoglobulin enhancer binding protein indometacin phosphotransferase inhibitor prostaglandin synthase sulfalazine enzyme inhibitor I kappa B I kappa B kinase immunoglobulin enhancer binding protein indometacin prostaglandin synthase inhibitor protein serine threonine kinase salazosulfapyridine amnesia animal experiment animal tissue article assay brain controlled study crab enzyme activation enzyme inhibition long term memory male nonhuman priority journal training amnesia animal Brachyura chemically induced disorder drug antagonism drug effect escape behavior habituation memory metabolism physiology time Amnesia Animals Brachyura Brain Cyclooxygenase Inhibitors Enzyme Inhibitors Escape Reaction Habituation, Psychophysiologic I-kappa B Kinase I-kappa B Proteins Indomethacin Male Memory NF-kappa B Protein-Serine-Threonine Kinases Sulfasalazine Time Factors Evidence for the participation of Rel/NF-κB transcription factors in long-term memory has recently been reported in the context-signal learning paradigm of the crab Chasmagnathus, in which a high correlation between long-term memory formation and NF-κB activation was observed. Two components of the NF-κB pathway in the crab brain have now been identified by cross-immunoreactivity using mammalian antibodies for IκB-α and IκB kinase α. Furthermore, IκB kinase-like phosphotransferase activity, which was inhibited by the IκB kinase inhibitor sulfasalazine, was detected in brain extracts. We have evaluated the effect of sulfasalazine administration on long-term memory tested at 48 h. Amnesia was found when sulfasalazine was administered pre-training and 5 h after training but not at 0 or 24 h after training. Thus, two periods for sulfasalazine-induced amnesia were found in coincidence with the two phases of NF-κB activation previously described (immediately and 6 h after training). The cyclooxygenase inhibitor indomethacin did not induce amnesia when administered pre-training. Thus, the possibility that sulfasalazine induces amnesia by means of cyclooxygenase inhibition is unlikely to be tenable. In vivo sulfasalazine inhibition of basal NF-κB activity was found between 30 and 45 min after injection, as assessed by electrophoretic mobility shift assay. On the other hand, in vivo sulfasalazine administration 6 h after training inhibited the second phase of training-induced NF-κB activation, providing evidence that the sulfasalazine effect on memory is due to a direct effect of the drug on the NF-κB pathway. These results provide the first evidence that IκB kinase and NF-κB activation are necessary for memory formation. © 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v112_n1_p161_Merlo http://hdl.handle.net/20.500.12110/paper_03064522_v112_n1_p161_Merlo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Crab IκB kinase long-term memory Neural plasticity Rel/NF-κB I kappa B kinase immunoglobulin enhancer binding protein indometacin phosphotransferase inhibitor prostaglandin synthase sulfalazine enzyme inhibitor I kappa B I kappa B kinase immunoglobulin enhancer binding protein indometacin prostaglandin synthase inhibitor protein serine threonine kinase salazosulfapyridine amnesia animal experiment animal tissue article assay brain controlled study crab enzyme activation enzyme inhibition long term memory male nonhuman priority journal training amnesia animal Brachyura chemically induced disorder drug antagonism drug effect escape behavior habituation memory metabolism physiology time Amnesia Animals Brachyura Brain Cyclooxygenase Inhibitors Enzyme Inhibitors Escape Reaction Habituation, Psychophysiologic I-kappa B Kinase I-kappa B Proteins Indomethacin Male Memory NF-kappa B Protein-Serine-Threonine Kinases Sulfasalazine Time Factors |
| spellingShingle |
Crab IκB kinase long-term memory Neural plasticity Rel/NF-κB I kappa B kinase immunoglobulin enhancer binding protein indometacin phosphotransferase inhibitor prostaglandin synthase sulfalazine enzyme inhibitor I kappa B I kappa B kinase immunoglobulin enhancer binding protein indometacin prostaglandin synthase inhibitor protein serine threonine kinase salazosulfapyridine amnesia animal experiment animal tissue article assay brain controlled study crab enzyme activation enzyme inhibition long term memory male nonhuman priority journal training amnesia animal Brachyura chemically induced disorder drug antagonism drug effect escape behavior habituation memory metabolism physiology time Amnesia Animals Brachyura Brain Cyclooxygenase Inhibitors Enzyme Inhibitors Escape Reaction Habituation, Psychophysiologic I-kappa B Kinase I-kappa B Proteins Indomethacin Male Memory NF-kappa B Protein-Serine-Threonine Kinases Sulfasalazine Time Factors The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus |
| topic_facet |
Crab IκB kinase long-term memory Neural plasticity Rel/NF-κB I kappa B kinase immunoglobulin enhancer binding protein indometacin phosphotransferase inhibitor prostaglandin synthase sulfalazine enzyme inhibitor I kappa B I kappa B kinase immunoglobulin enhancer binding protein indometacin prostaglandin synthase inhibitor protein serine threonine kinase salazosulfapyridine amnesia animal experiment animal tissue article assay brain controlled study crab enzyme activation enzyme inhibition long term memory male nonhuman priority journal training amnesia animal Brachyura chemically induced disorder drug antagonism drug effect escape behavior habituation memory metabolism physiology time Amnesia Animals Brachyura Brain Cyclooxygenase Inhibitors Enzyme Inhibitors Escape Reaction Habituation, Psychophysiologic I-kappa B Kinase I-kappa B Proteins Indomethacin Male Memory NF-kappa B Protein-Serine-Threonine Kinases Sulfasalazine Time Factors |
| description |
Evidence for the participation of Rel/NF-κB transcription factors in long-term memory has recently been reported in the context-signal learning paradigm of the crab Chasmagnathus, in which a high correlation between long-term memory formation and NF-κB activation was observed. Two components of the NF-κB pathway in the crab brain have now been identified by cross-immunoreactivity using mammalian antibodies for IκB-α and IκB kinase α. Furthermore, IκB kinase-like phosphotransferase activity, which was inhibited by the IκB kinase inhibitor sulfasalazine, was detected in brain extracts. We have evaluated the effect of sulfasalazine administration on long-term memory tested at 48 h. Amnesia was found when sulfasalazine was administered pre-training and 5 h after training but not at 0 or 24 h after training. Thus, two periods for sulfasalazine-induced amnesia were found in coincidence with the two phases of NF-κB activation previously described (immediately and 6 h after training). The cyclooxygenase inhibitor indomethacin did not induce amnesia when administered pre-training. Thus, the possibility that sulfasalazine induces amnesia by means of cyclooxygenase inhibition is unlikely to be tenable. In vivo sulfasalazine inhibition of basal NF-κB activity was found between 30 and 45 min after injection, as assessed by electrophoretic mobility shift assay. On the other hand, in vivo sulfasalazine administration 6 h after training inhibited the second phase of training-induced NF-κB activation, providing evidence that the sulfasalazine effect on memory is due to a direct effect of the drug on the NF-κB pathway. These results provide the first evidence that IκB kinase and NF-κB activation are necessary for memory formation. © 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved. |
| title |
The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus |
| title_short |
The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus |
| title_full |
The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus |
| title_fullStr |
The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus |
| title_full_unstemmed |
The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus |
| title_sort |
iκb kinase inhibitor sulfasalazine impairs long-term memory in the crab chasmagnathus |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v112_n1_p161_Merlo http://hdl.handle.net/20.500.12110/paper_03064522_v112_n1_p161_Merlo |
| _version_ |
1768541610833870848 |