Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids whi...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v112_n7_p1253_LopezFernandez http://hdl.handle.net/20.500.12110/paper_03057364_v112_n7_p1253_LopezFernandez |
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paper:paper_03057364_v112_n7_p1253_LopezFernandez |
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paper:paper_03057364_v112_n7_p1253_LopezFernandez2023-06-08T15:30:50Z Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids while the rest are crushed and disintegrated. Both the suspensor and endosperm die progressively from the innermost layers surrounding the embryo and extending towards the nucellar tissue. Ricinosomes are endoplasmic reticulum-derived organelles that accumulate both the pro-form and the mature form of cysteine endopeptidase (Cys-EP), first identified in castor bean (Ricinus communis) endosperm during germination. This study sought to identify associations between the presence of ricinosomes and programmed cell death (PCD) hallmarks in suspensor and endosperm cells predestined to die during quinoa seed development. MethodsAstructural study using light microscopy and transmission electron microscopywas performed. To detect the presence of Cys-EP, both western blot and in situ immunolocalization assays were carried out using anti- R. communis Cys-EP antibody. ATUNEL assay was used to determine DNA fragmentation. Results and Conclusions Except for the one or two cell layers that constitute the lasting endosperm in the mature seed, ricinosomes were found in suspensor and endosperm cells. These cells were also the site of morphological abnormalities, including misshapen and fragmented nuclei, vesiculation of the cytosol, vacuole collapse and cell wall disorganization. It is proposed that, in suspensor and endosperm cells, the early detection of Cys-EP in ricinosomes predicts the occurrence of PCD during late seed development. © The Author 2013. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v112_n7_p1253_LopezFernandez http://hdl.handle.net/20.500.12110/paper_03057364_v112_n7_p1253_LopezFernandez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions |
| spellingShingle |
Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) |
| topic_facet |
Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions |
| description |
Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids while the rest are crushed and disintegrated. Both the suspensor and endosperm die progressively from the innermost layers surrounding the embryo and extending towards the nucellar tissue. Ricinosomes are endoplasmic reticulum-derived organelles that accumulate both the pro-form and the mature form of cysteine endopeptidase (Cys-EP), first identified in castor bean (Ricinus communis) endosperm during germination. This study sought to identify associations between the presence of ricinosomes and programmed cell death (PCD) hallmarks in suspensor and endosperm cells predestined to die during quinoa seed development. MethodsAstructural study using light microscopy and transmission electron microscopywas performed. To detect the presence of Cys-EP, both western blot and in situ immunolocalization assays were carried out using anti- R. communis Cys-EP antibody. ATUNEL assay was used to determine DNA fragmentation. Results and Conclusions Except for the one or two cell layers that constitute the lasting endosperm in the mature seed, ricinosomes were found in suspensor and endosperm cells. These cells were also the site of morphological abnormalities, including misshapen and fragmented nuclei, vesiculation of the cytosol, vacuole collapse and cell wall disorganization. It is proposed that, in suspensor and endosperm cells, the early detection of Cys-EP in ricinosomes predicts the occurrence of PCD during late seed development. © The Author 2013. |
| title |
Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) |
| title_short |
Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) |
| title_full |
Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) |
| title_fullStr |
Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) |
| title_full_unstemmed |
Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) |
| title_sort |
ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (chenopodium quinoa) |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v112_n7_p1253_LopezFernandez http://hdl.handle.net/20.500.12110/paper_03057364_v112_n7_p1253_LopezFernandez |
| _version_ |
1768544039870660608 |