Effects of photooxidation on membrane integrity in Salix nigra seeds
Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. T...
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2010
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paper:paper_03057364_v105_n6_p1027_Roqueiro2023-06-08T15:30:50Z Effects of photooxidation on membrane integrity in Salix nigra seeds Roqueiro, Gonzalo Maroder, Horacio Luis Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v105_n6_p1027_Roqueiro http://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_Roqueiro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra |
| spellingShingle |
Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra Roqueiro, Gonzalo Maroder, Horacio Luis Effects of photooxidation on membrane integrity in Salix nigra seeds |
| topic_facet |
Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds chlorophyll oxygen chlorophyll chloroplast deciduous tree desiccation embryo fatty acid free radical germination light intensity lipid liquid chromatography membrane permeability photooxidation seed spectroscopy temperature effect tolerance transmission electron microscopy adaptation article light membrane metabolism methodology nuclear magnetic resonance spectroscopy oxidation reduction reaction photochemistry physiology plant seed radiation exposure willow Adaptation, Physiological Chlorophyll Light Magnetic Resonance Spectroscopy Membranes Oxidation-Reduction Oxygen Photochemistry Salix Seeds Salicaceae Salix Salix nigra Salicaceae Salix Salix nigra |
| description |
Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation. © The Author 2010. |
| author |
Roqueiro, Gonzalo Maroder, Horacio Luis |
| author_facet |
Roqueiro, Gonzalo Maroder, Horacio Luis |
| author_sort |
Roqueiro, Gonzalo |
| title |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
| title_short |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
| title_full |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
| title_fullStr |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
| title_full_unstemmed |
Effects of photooxidation on membrane integrity in Salix nigra seeds |
| title_sort |
effects of photooxidation on membrane integrity in salix nigra seeds |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v105_n6_p1027_Roqueiro http://hdl.handle.net/20.500.12110/paper_03057364_v105_n6_p1027_Roqueiro |
| work_keys_str_mv |
AT roqueirogonzalo effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT maroderhoracioluis effectsofphotooxidationonmembraneintegrityinsalixnigraseeds |
| _version_ |
1768543800174575616 |