First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes

Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. Accord...

Descripción completa

Detalles Bibliográficos
Autores principales:	Craig, María Isabel, Romera, Sonia Alejandra
Publicado:	2014
Materias:	Bovine herpesvirus 1 Bubaline herpesvirus 1 Bubalus Bubalus bubalis Alphaherpesvirinae animal Argentina buffalo classification genetics herpes virus infection isolation and purification molecular genetics phylogeny veterinary virology Animals Buffaloes Herpesviridae Infections Molecular Sequence Data Phylogeny
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v159_n11_p2917_Maidana http://hdl.handle.net/20.500.12110/paper_03048608_v159_n11_p2917_Maidana
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03048608_v159_n11_p2917_Maidana
record_format	dspace
spelling	paper:paper_03048608_v159_n11_p2917_Maidana2023-06-08T15:30:06Z First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes Craig, María Isabel Romera, Sonia Alejandra Bovine herpesvirus 1 Bubaline herpesvirus 1 Bubalus Bubalus bubalis Alphaherpesvirinae animal Argentina buffalo classification genetics herpes virus infection isolation and purification molecular genetics phylogeny veterinary virology Alphaherpesvirinae Animals Argentina Buffaloes Herpesviridae Infections Molecular Sequence Data Phylogeny Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. © 2014, Springer-Verlag Wien. Fil:Craig, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romera, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v159_n11_p2917_Maidana http://hdl.handle.net/20.500.12110/paper_03048608_v159_n11_p2917_Maidana
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bovine herpesvirus 1 Bubaline herpesvirus 1 Bubalus Bubalus bubalis Alphaherpesvirinae animal Argentina buffalo classification genetics herpes virus infection isolation and purification molecular genetics phylogeny veterinary virology Alphaherpesvirinae Animals Argentina Buffaloes Herpesviridae Infections Molecular Sequence Data Phylogeny
spellingShingle	Bovine herpesvirus 1 Bubaline herpesvirus 1 Bubalus Bubalus bubalis Alphaherpesvirinae animal Argentina buffalo classification genetics herpes virus infection isolation and purification molecular genetics phylogeny veterinary virology Alphaherpesvirinae Animals Argentina Buffaloes Herpesviridae Infections Molecular Sequence Data Phylogeny Craig, María Isabel Romera, Sonia Alejandra First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
topic_facet	Bovine herpesvirus 1 Bubaline herpesvirus 1 Bubalus Bubalus bubalis Alphaherpesvirinae animal Argentina buffalo classification genetics herpes virus infection isolation and purification molecular genetics phylogeny veterinary virology Alphaherpesvirinae Animals Argentina Buffaloes Herpesviridae Infections Molecular Sequence Data Phylogeny
description	Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. © 2014, Springer-Verlag Wien.
author	Craig, María Isabel Romera, Sonia Alejandra
author_facet	Craig, María Isabel Romera, Sonia Alejandra
author_sort	Craig, María Isabel
title	First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_short	First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_full	First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_fullStr	First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_full_unstemmed	First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
title_sort	first report of isolation and molecular characterization of bubaline herpesvirus 1 (buhv1) from argentinean water buffaloes
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v159_n11_p2917_Maidana http://hdl.handle.net/20.500.12110/paper_03048608_v159_n11_p2917_Maidana
work_keys_str_mv	AT craigmariaisabel firstreportofisolationandmolecularcharacterizationofbubalineherpesvirus1buhv1fromargentineanwaterbuffaloes AT romerasoniaalejandra firstreportofisolationandmolecularcharacterizationofbubalineherpesvirus1buhv1fromargentineanwaterbuffaloes
_version_	1768545051454996480

First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes

Ejemplares similares