Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid
To study the functional involvement of cellular membrane properties on arenavirus infection, saturated fatty acids of variable chain length (C10-C18) were evaluated for their inhibitory activity against the multiplication of Junin virus (JUNV). The most active inhibitor was lauric acid (C12), which...
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2001
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paper:paper_03048608_v146_n4_p777_Bartolotta2023-06-08T15:30:04Z Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid García, Cybele Carina Candurra, Nélida Alicia Damonte, Elsa Beatriz Arenavirus cell membrane cell stimulation Junin virus lauric acid saturated fatty acid triacylglycerol virion virus glycoprotein virus infection virus protein virus replication Animals Cell Line Cercopithecus aethiops Dose-Response Relationship, Drug Fatty Acids Inhibitory Concentration 50 Junin virus Lauric Acids Membrane Glycoproteins Microbial Sensitivity Tests Triglycerides Vero Cells Viral Proteins Virion Virus Replication Arenavirus Junin virus To study the functional involvement of cellular membrane properties on arenavirus infection, saturated fatty acids of variable chain length (C10-C18) were evaluated for their inhibitory activity against the multiplication of Junin virus (JUNV). The most active inhibitor was lauric acid (C12), which reduced virus yields of several attenuated and pathogenic strains of JUNV in a dose dependent manner, without affecting cell viability. Fatty acids with shorter or longer chain length had a reduced or negligible anti-JUNV activity. Lauric acid did not inactivate virion infectivity neither interacted with the cell to induce a state refractory to virus infection. From mechanistic studies, it can be concluded that lauric acid inhibited a late maturation stage in the replicative cycle of JUNV. Viral protein synthesis was not affected by the compound, but the expression of glycoproteins in the plasma membrane was diminished. A direct correlation between the inhibition of JUNV production and the stimulation of triacylglycerol cell content was demonstrated, and both lauric-acid induced effects were dependent on the continued presence of the fatty acid. Thus, the decreased insertion of viral glycoproteins into the plasma membrane, apparently due to the increased incorporation of triacylglycerols, seems to cause an inhibition of JUNV maturation and release. Fil:García, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v146_n4_p777_Bartolotta http://hdl.handle.net/20.500.12110/paper_03048608_v146_n4_p777_Bartolotta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arenavirus cell membrane cell stimulation Junin virus lauric acid saturated fatty acid triacylglycerol virion virus glycoprotein virus infection virus protein virus replication Animals Cell Line Cercopithecus aethiops Dose-Response Relationship, Drug Fatty Acids Inhibitory Concentration 50 Junin virus Lauric Acids Membrane Glycoproteins Microbial Sensitivity Tests Triglycerides Vero Cells Viral Proteins Virion Virus Replication Arenavirus Junin virus |
| spellingShingle |
Arenavirus cell membrane cell stimulation Junin virus lauric acid saturated fatty acid triacylglycerol virion virus glycoprotein virus infection virus protein virus replication Animals Cell Line Cercopithecus aethiops Dose-Response Relationship, Drug Fatty Acids Inhibitory Concentration 50 Junin virus Lauric Acids Membrane Glycoproteins Microbial Sensitivity Tests Triglycerides Vero Cells Viral Proteins Virion Virus Replication Arenavirus Junin virus García, Cybele Carina Candurra, Nélida Alicia Damonte, Elsa Beatriz Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid |
| topic_facet |
Arenavirus cell membrane cell stimulation Junin virus lauric acid saturated fatty acid triacylglycerol virion virus glycoprotein virus infection virus protein virus replication Animals Cell Line Cercopithecus aethiops Dose-Response Relationship, Drug Fatty Acids Inhibitory Concentration 50 Junin virus Lauric Acids Membrane Glycoproteins Microbial Sensitivity Tests Triglycerides Vero Cells Viral Proteins Virion Virus Replication Arenavirus Junin virus |
| description |
To study the functional involvement of cellular membrane properties on arenavirus infection, saturated fatty acids of variable chain length (C10-C18) were evaluated for their inhibitory activity against the multiplication of Junin virus (JUNV). The most active inhibitor was lauric acid (C12), which reduced virus yields of several attenuated and pathogenic strains of JUNV in a dose dependent manner, without affecting cell viability. Fatty acids with shorter or longer chain length had a reduced or negligible anti-JUNV activity. Lauric acid did not inactivate virion infectivity neither interacted with the cell to induce a state refractory to virus infection. From mechanistic studies, it can be concluded that lauric acid inhibited a late maturation stage in the replicative cycle of JUNV. Viral protein synthesis was not affected by the compound, but the expression of glycoproteins in the plasma membrane was diminished. A direct correlation between the inhibition of JUNV production and the stimulation of triacylglycerol cell content was demonstrated, and both lauric-acid induced effects were dependent on the continued presence of the fatty acid. Thus, the decreased insertion of viral glycoproteins into the plasma membrane, apparently due to the increased incorporation of triacylglycerols, seems to cause an inhibition of JUNV maturation and release. |
| author |
García, Cybele Carina Candurra, Nélida Alicia Damonte, Elsa Beatriz |
| author_facet |
García, Cybele Carina Candurra, Nélida Alicia Damonte, Elsa Beatriz |
| author_sort |
García, Cybele Carina |
| title |
Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid |
| title_short |
Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid |
| title_full |
Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid |
| title_fullStr |
Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid |
| title_full_unstemmed |
Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid |
| title_sort |
effect of fatty acids on arenavirus replication: inhibition of virus production by lauric acid |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v146_n4_p777_Bartolotta http://hdl.handle.net/20.500.12110/paper_03048608_v146_n4_p777_Bartolotta |
| work_keys_str_mv |
AT garciacybelecarina effectoffattyacidsonarenavirusreplicationinhibitionofvirusproductionbylauricacid AT candurranelidaalicia effectoffattyacidsonarenavirusreplicationinhibitionofvirusproductionbylauricacid AT damonteelsabeatriz effectoffattyacidsonarenavirusreplicationinhibitionofvirusproductionbylauricacid |
| _version_ |
1768542219790188544 |