Glycogen synthesis in the fungus Neurospora crassa

An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen...

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Autor principal:	Fontana, José Domingo
Publicado:	1978
Materias:	glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v540_n2_p183_Fontana http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03044165_v540_n2_p183_Fontana
record_format	dspace
spelling	paper:paper_03044165_v540_n2_p183_Fontana2025-07-30T18:07:33Z Glycogen synthesis in the fungus Neurospora crassa Fontana, José Domingo glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978. Fil:Fontana, J.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v540_n2_p183_Fontana http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose
spellingShingle	glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose Fontana, José Domingo Glycogen synthesis in the fungus Neurospora crassa
topic_facet	glycogen radioisotope adenosine diphosphate glucose c 14 animal experiment fungus glucose c 14 in vitro study neurospora crassa Adenosine Diphosphate Glucose Comparative Study Enzyme Activation Glycogen Synthase Neurospora Neurospora crassa Uridine Diphosphate Glucose
description	An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978.
author	Fontana, José Domingo
author_facet	Fontana, José Domingo
author_sort	Fontana, José Domingo
title	Glycogen synthesis in the fungus Neurospora crassa
title_short	Glycogen synthesis in the fungus Neurospora crassa
title_full	Glycogen synthesis in the fungus Neurospora crassa
title_fullStr	Glycogen synthesis in the fungus Neurospora crassa
title_full_unstemmed	Glycogen synthesis in the fungus Neurospora crassa
title_sort	glycogen synthesis in the fungus neurospora crassa
publishDate	1978
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v540_n2_p183_Fontana http://hdl.handle.net/20.500.12110/paper_03044165_v540_n2_p183_Fontana
work_keys_str_mv	AT fontanajosedomingo glycogensynthesisinthefungusneurosporacrassa
_version_	1840321430180331520

Glycogen synthesis in the fungus Neurospora crassa

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