Nucleotides and nucleotide sugars in human blood cells. II. Group "O"

A study of nucleotides and nucleotide sugars in human blood cells has been carried out. Ten samples from healthy yound people of group "O" were analyzed by ion-exchange chromatography, and the following nucleotides and nucleotide sugars were found: NAD+; UDP-glucose, UDP-mannose, UDP-galac...

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Autores principales:	Cantore de Ezcurra, María Leonor, Leoni, Patricia R. D., Recondo, Eduardo Francisco
Publicado:	1971
Materias:	adenine nucleotide alcohol ammonium chloride carbon chloroform fucose galactose glucosamine guanine nucleotide hexosamine hexose mannose nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate nucleoside diphosphate sugar nucleotide pyrimidine nucleotide trichloroacetic acid adult article blood blood cell blood group ABO system electrophoresis glucose blood level human ion exchange chromatography isolation and purification paper paper chromatography pH spectrophotometry technique ultraviolet radiation ABO Blood-Group System Adenine Nucleotides Adult Ammonium Chloride Blood Cells Blood Glucose Carbon Isotopes Chloroform Chromatography, Ion Exchange Chromatography, Paper Electrophoresis Ethanol Fucose Galactose Glucosamine Guanine Nucleotides Hexosamines Hexoses Human Hydrogen-Ion Concentration Mannose Methods NAD NADP Nucleoside Diphosphate Sugars Nucleotides Paper Spectrophotometry Trichloroacetic Acid Ultraviolet Rays Uracil Nucleotides
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v230_n3_p423_Cantore http://hdl.handle.net/20.500.12110/paper_03044165_v230_n3_p423_Cantore
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_03044165_v230_n3_p423_Cantore2023-06-08T15:29:54Z Nucleotides and nucleotide sugars in human blood cells. II. Group "O" Cantore de Ezcurra, María Leonor Leoni, Patricia R. D. Recondo, Eduardo Francisco adenine nucleotide alcohol ammonium chloride carbon chloroform fucose galactose glucosamine guanine nucleotide hexosamine hexose mannose nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate nucleoside diphosphate sugar nucleotide pyrimidine nucleotide trichloroacetic acid adult article blood blood cell blood group ABO system electrophoresis glucose blood level human ion exchange chromatography isolation and purification paper paper chromatography pH spectrophotometry technique ultraviolet radiation ABO Blood-Group System Adenine Nucleotides Adult Ammonium Chloride Blood Cells Blood Glucose Carbon Isotopes Chloroform Chromatography, Ion Exchange Chromatography, Paper Electrophoresis Ethanol Fucose Galactose Glucosamine Guanine Nucleotides Hexosamines Hexoses Human Hydrogen-Ion Concentration Mannose Methods NAD NADP Nucleoside Diphosphate Sugars Nucleotides Paper Spectrophotometry Trichloroacetic Acid Ultraviolet Rays Uracil Nucleotides A study of nucleotides and nucleotide sugars in human blood cells has been carried out. Ten samples from healthy yound people of group "O" were analyzed by ion-exchange chromatography, and the following nucleotides and nucleotide sugars were found: NAD+; UDP-glucose, UDP-mannose, UDP-galactose, UDP-acetylglucosamine and UDP-acetylgalactosamine; AMP; NADP+; ADP-glucose, ADP-galactose and ADP-mannose; GDP-mannose and GDP-fucose; ADP and ATP. The sugar nucleotides were identified on the basis of chemical, enzymatic and chromatographic analysis. UDP-galactose, UDP-mannose, UDP-acetylgalactosamine, ADP-glucose, ADP-galactose, ADP-mannose, GDP-mannose and GDP-fucose have not been found before in human blood, and UDP-mannose was not known to occur in nature. In order to minimize the decomposition of unstable substances, mild techniques were applied: cold acid extraction and neutral (ethanol-chloroform) extraction were compared, and in both cases the aqueous phase was passed through a column of Dowex-i (Cl-form) resin, and the nucleotides eluted with a linear gradient of NH4Cl. Results showed that the extraction step is not very critical, but the use of ammonium salts and almost neutral pH, as compared with methods used in previous papers, permitted the isolation of ten nucleotide-linked sugars, eight of which have not been described before in human blood. An adenine nucleotide with very particular properties was extracted with the ethanol-chloroform technique; it was eluted by the concentration of NH4Cl necessary to elute ATP, but it behaved as AMP on paper chromatography in several solvent systems. It is very unstable and decomposes even at -20° in a few days. © 1971. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leoni, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Recondo, E.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1971 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v230_n3_p423_Cantore http://hdl.handle.net/20.500.12110/paper_03044165_v230_n3_p423_Cantore
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenine nucleotide alcohol ammonium chloride carbon chloroform fucose galactose glucosamine guanine nucleotide hexosamine hexose mannose nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate nucleoside diphosphate sugar nucleotide pyrimidine nucleotide trichloroacetic acid adult article blood blood cell blood group ABO system electrophoresis glucose blood level human ion exchange chromatography isolation and purification paper paper chromatography pH spectrophotometry technique ultraviolet radiation ABO Blood-Group System Adenine Nucleotides Adult Ammonium Chloride Blood Cells Blood Glucose Carbon Isotopes Chloroform Chromatography, Ion Exchange Chromatography, Paper Electrophoresis Ethanol Fucose Galactose Glucosamine Guanine Nucleotides Hexosamines Hexoses Human Hydrogen-Ion Concentration Mannose Methods NAD NADP Nucleoside Diphosphate Sugars Nucleotides Paper Spectrophotometry Trichloroacetic Acid Ultraviolet Rays Uracil Nucleotides
spellingShingle	adenine nucleotide alcohol ammonium chloride carbon chloroform fucose galactose glucosamine guanine nucleotide hexosamine hexose mannose nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate nucleoside diphosphate sugar nucleotide pyrimidine nucleotide trichloroacetic acid adult article blood blood cell blood group ABO system electrophoresis glucose blood level human ion exchange chromatography isolation and purification paper paper chromatography pH spectrophotometry technique ultraviolet radiation ABO Blood-Group System Adenine Nucleotides Adult Ammonium Chloride Blood Cells Blood Glucose Carbon Isotopes Chloroform Chromatography, Ion Exchange Chromatography, Paper Electrophoresis Ethanol Fucose Galactose Glucosamine Guanine Nucleotides Hexosamines Hexoses Human Hydrogen-Ion Concentration Mannose Methods NAD NADP Nucleoside Diphosphate Sugars Nucleotides Paper Spectrophotometry Trichloroacetic Acid Ultraviolet Rays Uracil Nucleotides Cantore de Ezcurra, María Leonor Leoni, Patricia R. D. Recondo, Eduardo Francisco Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
topic_facet	adenine nucleotide alcohol ammonium chloride carbon chloroform fucose galactose glucosamine guanine nucleotide hexosamine hexose mannose nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate nucleoside diphosphate sugar nucleotide pyrimidine nucleotide trichloroacetic acid adult article blood blood cell blood group ABO system electrophoresis glucose blood level human ion exchange chromatography isolation and purification paper paper chromatography pH spectrophotometry technique ultraviolet radiation ABO Blood-Group System Adenine Nucleotides Adult Ammonium Chloride Blood Cells Blood Glucose Carbon Isotopes Chloroform Chromatography, Ion Exchange Chromatography, Paper Electrophoresis Ethanol Fucose Galactose Glucosamine Guanine Nucleotides Hexosamines Hexoses Human Hydrogen-Ion Concentration Mannose Methods NAD NADP Nucleoside Diphosphate Sugars Nucleotides Paper Spectrophotometry Trichloroacetic Acid Ultraviolet Rays Uracil Nucleotides
description	A study of nucleotides and nucleotide sugars in human blood cells has been carried out. Ten samples from healthy yound people of group "O" were analyzed by ion-exchange chromatography, and the following nucleotides and nucleotide sugars were found: NAD+; UDP-glucose, UDP-mannose, UDP-galactose, UDP-acetylglucosamine and UDP-acetylgalactosamine; AMP; NADP+; ADP-glucose, ADP-galactose and ADP-mannose; GDP-mannose and GDP-fucose; ADP and ATP. The sugar nucleotides were identified on the basis of chemical, enzymatic and chromatographic analysis. UDP-galactose, UDP-mannose, UDP-acetylgalactosamine, ADP-glucose, ADP-galactose, ADP-mannose, GDP-mannose and GDP-fucose have not been found before in human blood, and UDP-mannose was not known to occur in nature. In order to minimize the decomposition of unstable substances, mild techniques were applied: cold acid extraction and neutral (ethanol-chloroform) extraction were compared, and in both cases the aqueous phase was passed through a column of Dowex-i (Cl-form) resin, and the nucleotides eluted with a linear gradient of NH4Cl. Results showed that the extraction step is not very critical, but the use of ammonium salts and almost neutral pH, as compared with methods used in previous papers, permitted the isolation of ten nucleotide-linked sugars, eight of which have not been described before in human blood. An adenine nucleotide with very particular properties was extracted with the ethanol-chloroform technique; it was eluted by the concentration of NH4Cl necessary to elute ATP, but it behaved as AMP on paper chromatography in several solvent systems. It is very unstable and decomposes even at -20° in a few days. © 1971.
author	Cantore de Ezcurra, María Leonor Leoni, Patricia R. D. Recondo, Eduardo Francisco
author_facet	Cantore de Ezcurra, María Leonor Leoni, Patricia R. D. Recondo, Eduardo Francisco
author_sort	Cantore de Ezcurra, María Leonor
title	Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
title_short	Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
title_full	Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
title_fullStr	Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
title_full_unstemmed	Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
title_sort	nucleotides and nucleotide sugars in human blood cells. ii. group "o"
publishDate	1971
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v230_n3_p423_Cantore http://hdl.handle.net/20.500.12110/paper_03044165_v230_n3_p423_Cantore
work_keys_str_mv	AT cantoredeezcurramarialeonor nucleotidesandnucleotidesugarsinhumanbloodcellsiigroupo AT leonipatriciard nucleotidesandnucleotidesugarsinhumanbloodcellsiigroupo AT recondoeduardofrancisco nucleotidesandnucleotidesugarsinhumanbloodcellsiigroupo
_version_	1768544180754186240

Nucleotides and nucleotide sugars in human blood cells. II. Group "O"

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