When size does matter: Organelle size influences the properties of transport mediated by molecular motors

Background Organelle transport is driven by the action of molecular motors. In this work, we studied the dynamics of organelles of different sizes with the aim of understanding the complex relation between organelle motion and microenvironment. Methods We used single particle tracking to obtain traj...

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Autores principales:	Bruno, Luciana, Despósito, Marcelo Arnaldo, Levi, Valeria
Publicado:	2013
Materias:	Intracellular transport Molecular motors Organelle trafficking Single particle tracking Xenopus laevis melanophores- actin molecular motor animal cell article cell aggregation cell tracking cell transport controlled study depolymerization diffusion dispersion intermediate filament melanosome nonhuman organelle size priority journal Xenopus laevis anomalous diffusion exponent DLS dynamic light scattering FE-SEM field emission-scanning electron microscopy IF intermediate filaments mean square displacement MSD optical radius OR Xenopus laevis melanophores α Actins Animals Biological Transport Cells, Cultured Cellular Microenvironment Diffusion Dyneins Intermediate Filaments Melanophores Melanosomes Microtubules Molecular Motor Proteins Organelle Size Organelles Structure-Activity Relationship
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1830_n11_p5095_DeRossi http://hdl.handle.net/20.500.12110/paper_03044165_v1830_n11_p5095_DeRossi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03044165_v1830_n11_p5095_DeRossi
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spelling	paper:paper_03044165_v1830_n11_p5095_DeRossi2023-06-08T15:29:52Z When size does matter: Organelle size influences the properties of transport mediated by molecular motors Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria Intracellular transport Molecular motors Organelle trafficking Single particle tracking Xenopus laevis melanophores- actin molecular motor animal cell article cell aggregation cell tracking cell transport controlled study depolymerization diffusion dispersion intermediate filament melanosome nonhuman organelle size priority journal Xenopus laevis anomalous diffusion exponent DLS dynamic light scattering FE-SEM field emission-scanning electron microscopy IF intermediate filaments Intracellular transport mean square displacement Molecular motors MSD optical radius OR Organelle trafficking Single particle tracking Xenopus laevis melanophores α Actins Animals Biological Transport Cells, Cultured Cellular Microenvironment Diffusion Dyneins Intermediate Filaments Melanophores Melanosomes Microtubules Molecular Motor Proteins Organelle Size Organelles Structure-Activity Relationship Xenopus laevis Background Organelle transport is driven by the action of molecular motors. In this work, we studied the dynamics of organelles of different sizes with the aim of understanding the complex relation between organelle motion and microenvironment. Methods We used single particle tracking to obtain trajectories of melanosomes (pigmented organelles in Xenopus laevis melanophores). In response to certain hormones, melanosomes disperse in the cytoplasm or aggregate in the perinuclear region by the combined action of microtubule and actin motors. Results and conclusions Melanosome trajectories followed an anomalous diffusion model in which the anomalous diffusion exponent (α) provided information regarding the trajectories' topography and thus of the processes causing it. During aggregation, the directionality of big organelles was higher than that of small organelles and did not depend on the presence of either actin or intermediate filaments (IF). Depolymerization of IF significantly reduced α values of small organelles during aggregation but slightly affect their directionality during dispersion. General significance Our results could be interpreted considering that the number of copies of active motors increases with organelle size. Transport of big organelles was not influenced by actin or IF during aggregation showing that these organelles are moved processively by the collective action of dynein motors. Also, we found that intermediate filaments enhance the directionality of small organelles suggesting that this network keeps organelles close to the tracks allowing their efficient reattachment. The higher directionality of small organelles during dispersion could be explained considering the better performance of kinesin-2 vs. dynein at the single molecule level. © 2013 Elsevier B.V. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1830_n11_p5095_DeRossi http://hdl.handle.net/20.500.12110/paper_03044165_v1830_n11_p5095_DeRossi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Intracellular transport Molecular motors Organelle trafficking Single particle tracking Xenopus laevis melanophores- actin molecular motor animal cell article cell aggregation cell tracking cell transport controlled study depolymerization diffusion dispersion intermediate filament melanosome nonhuman organelle size priority journal Xenopus laevis anomalous diffusion exponent DLS dynamic light scattering FE-SEM field emission-scanning electron microscopy IF intermediate filaments Intracellular transport mean square displacement Molecular motors MSD optical radius OR Organelle trafficking Single particle tracking Xenopus laevis melanophores α Actins Animals Biological Transport Cells, Cultured Cellular Microenvironment Diffusion Dyneins Intermediate Filaments Melanophores Melanosomes Microtubules Molecular Motor Proteins Organelle Size Organelles Structure-Activity Relationship Xenopus laevis
spellingShingle	Intracellular transport Molecular motors Organelle trafficking Single particle tracking Xenopus laevis melanophores- actin molecular motor animal cell article cell aggregation cell tracking cell transport controlled study depolymerization diffusion dispersion intermediate filament melanosome nonhuman organelle size priority journal Xenopus laevis anomalous diffusion exponent DLS dynamic light scattering FE-SEM field emission-scanning electron microscopy IF intermediate filaments Intracellular transport mean square displacement Molecular motors MSD optical radius OR Organelle trafficking Single particle tracking Xenopus laevis melanophores α Actins Animals Biological Transport Cells, Cultured Cellular Microenvironment Diffusion Dyneins Intermediate Filaments Melanophores Melanosomes Microtubules Molecular Motor Proteins Organelle Size Organelles Structure-Activity Relationship Xenopus laevis Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria When size does matter: Organelle size influences the properties of transport mediated by molecular motors
topic_facet	Intracellular transport Molecular motors Organelle trafficking Single particle tracking Xenopus laevis melanophores- actin molecular motor animal cell article cell aggregation cell tracking cell transport controlled study depolymerization diffusion dispersion intermediate filament melanosome nonhuman organelle size priority journal Xenopus laevis anomalous diffusion exponent DLS dynamic light scattering FE-SEM field emission-scanning electron microscopy IF intermediate filaments Intracellular transport mean square displacement Molecular motors MSD optical radius OR Organelle trafficking Single particle tracking Xenopus laevis melanophores α Actins Animals Biological Transport Cells, Cultured Cellular Microenvironment Diffusion Dyneins Intermediate Filaments Melanophores Melanosomes Microtubules Molecular Motor Proteins Organelle Size Organelles Structure-Activity Relationship Xenopus laevis
description	Background Organelle transport is driven by the action of molecular motors. In this work, we studied the dynamics of organelles of different sizes with the aim of understanding the complex relation between organelle motion and microenvironment. Methods We used single particle tracking to obtain trajectories of melanosomes (pigmented organelles in Xenopus laevis melanophores). In response to certain hormones, melanosomes disperse in the cytoplasm or aggregate in the perinuclear region by the combined action of microtubule and actin motors. Results and conclusions Melanosome trajectories followed an anomalous diffusion model in which the anomalous diffusion exponent (α) provided information regarding the trajectories' topography and thus of the processes causing it. During aggregation, the directionality of big organelles was higher than that of small organelles and did not depend on the presence of either actin or intermediate filaments (IF). Depolymerization of IF significantly reduced α values of small organelles during aggregation but slightly affect their directionality during dispersion. General significance Our results could be interpreted considering that the number of copies of active motors increases with organelle size. Transport of big organelles was not influenced by actin or IF during aggregation showing that these organelles are moved processively by the collective action of dynein motors. Also, we found that intermediate filaments enhance the directionality of small organelles suggesting that this network keeps organelles close to the tracks allowing their efficient reattachment. The higher directionality of small organelles during dispersion could be explained considering the better performance of kinesin-2 vs. dynein at the single molecule level. © 2013 Elsevier B.V.
author	Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria
author_facet	Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria
author_sort	Bruno, Luciana
title	When size does matter: Organelle size influences the properties of transport mediated by molecular motors
title_short	When size does matter: Organelle size influences the properties of transport mediated by molecular motors
title_full	When size does matter: Organelle size influences the properties of transport mediated by molecular motors
title_fullStr	When size does matter: Organelle size influences the properties of transport mediated by molecular motors
title_full_unstemmed	When size does matter: Organelle size influences the properties of transport mediated by molecular motors
title_sort	when size does matter: organelle size influences the properties of transport mediated by molecular motors
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1830_n11_p5095_DeRossi http://hdl.handle.net/20.500.12110/paper_03044165_v1830_n11_p5095_DeRossi
work_keys_str_mv	AT brunoluciana whensizedoesmatterorganellesizeinfluencesthepropertiesoftransportmediatedbymolecularmotors AT despositomarceloarnaldo whensizedoesmatterorganellesizeinfluencesthepropertiesoftransportmediatedbymolecularmotors AT levivaleria whensizedoesmatterorganellesizeinfluencesthepropertiesoftransportmediatedbymolecularmotors
_version_	1768544595330727936

When size does matter: Organelle size influences the properties of transport mediated by molecular motors

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