Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain
Due to its widespread incidence, maternal malnutrition remains one of the major non-genetic factors affecting the development of newborn's brain. While all nutrients have certain influence on brain maturation, proteins appear to be the most critical for the development of neurological functions...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v647_n_p38_Berardino http://hdl.handle.net/20.500.12110/paper_03043940_v647_n_p38_Berardino |
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paper:paper_03043940_v647_n_p38_Berardino2023-06-08T15:29:33Z Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain Cánepa, Eduardo Tomás Early life adversity Hippocampus Hypothalamus Mouse Neurodevelopment Undernutrition argonaute 2 protein exportin 5 microRNA argonaute protein EIF2C2 protein, mouse karyopherin microRNA Xpo5 protein, mouse animal tissue Article biogenesis brain brain weight controlled study female gene expression gestation period hippocampus hypertension hypothalamus insulin resistance kwashiorkor lactation male mouse nonhuman perinatal period pregnancy priority journal progeny protein restriction animal brain genetics maternal nutrition metabolism prenatal exposure protein deficiency protein restriction Animals Argonaute Proteins Brain Diet, Protein-Restricted Female Karyopherins Lactation Male Maternal Nutritional Physiological Phenomena Mice MicroRNAs Pregnancy Prenatal Exposure Delayed Effects Prenatal Nutritional Physiological Phenomena Protein Deficiency Due to its widespread incidence, maternal malnutrition remains one of the major non-genetic factors affecting the development of newborn's brain. While all nutrients have certain influence on brain maturation, proteins appear to be the most critical for the development of neurological functions. An increasing number of studies point out that the effects of early-life nutritional inadequacy has long lasting effects on the brain and lead to permanent deficits in learning and behavior. Epigenetic mechanisms provide a potential link between the nutrition status during critical periods and changes in gene expression that may lead to disease phenotypes. Among those epigenetic mechanisms microRNAs (miRNAs) emerge as promising molecules for the link between nutrition and gene expression due to their relevance in many central nervous system functions. The objective of the current study was to evaluate the impact of perinatal protein malnutrition on the development of male and female mice offspring and to analyze the expression of the genes involved in the miRNA biogenesis pathway in different mouse brain structures. We demonstrated that early nutritional stress such as exposition to a protein-deficient diet during gestation and lactation reduced the hippocampal weight, delayed offspring's development and deregulated the expression of Xpo5 and Ago2 genes in hippocampus and hypothalamus of weanling mice. Moreover, an overall increase in mature miRNAs was consistent with the induction of Xpo5 mRNA. Altered miRNA biogenesis could modify the availability and functionality of miRNA becoming a causal factor of the adverse effects of protein malnutrition. © 2017 Elsevier B.V. Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v647_n_p38_Berardino http://hdl.handle.net/20.500.12110/paper_03043940_v647_n_p38_Berardino |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Early life adversity Hippocampus Hypothalamus Mouse Neurodevelopment Undernutrition argonaute 2 protein exportin 5 microRNA argonaute protein EIF2C2 protein, mouse karyopherin microRNA Xpo5 protein, mouse animal tissue Article biogenesis brain brain weight controlled study female gene expression gestation period hippocampus hypertension hypothalamus insulin resistance kwashiorkor lactation male mouse nonhuman perinatal period pregnancy priority journal progeny protein restriction animal brain genetics maternal nutrition metabolism prenatal exposure protein deficiency protein restriction Animals Argonaute Proteins Brain Diet, Protein-Restricted Female Karyopherins Lactation Male Maternal Nutritional Physiological Phenomena Mice MicroRNAs Pregnancy Prenatal Exposure Delayed Effects Prenatal Nutritional Physiological Phenomena Protein Deficiency |
| spellingShingle |
Early life adversity Hippocampus Hypothalamus Mouse Neurodevelopment Undernutrition argonaute 2 protein exportin 5 microRNA argonaute protein EIF2C2 protein, mouse karyopherin microRNA Xpo5 protein, mouse animal tissue Article biogenesis brain brain weight controlled study female gene expression gestation period hippocampus hypertension hypothalamus insulin resistance kwashiorkor lactation male mouse nonhuman perinatal period pregnancy priority journal progeny protein restriction animal brain genetics maternal nutrition metabolism prenatal exposure protein deficiency protein restriction Animals Argonaute Proteins Brain Diet, Protein-Restricted Female Karyopherins Lactation Male Maternal Nutritional Physiological Phenomena Mice MicroRNAs Pregnancy Prenatal Exposure Delayed Effects Prenatal Nutritional Physiological Phenomena Protein Deficiency Cánepa, Eduardo Tomás Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain |
| topic_facet |
Early life adversity Hippocampus Hypothalamus Mouse Neurodevelopment Undernutrition argonaute 2 protein exportin 5 microRNA argonaute protein EIF2C2 protein, mouse karyopherin microRNA Xpo5 protein, mouse animal tissue Article biogenesis brain brain weight controlled study female gene expression gestation period hippocampus hypertension hypothalamus insulin resistance kwashiorkor lactation male mouse nonhuman perinatal period pregnancy priority journal progeny protein restriction animal brain genetics maternal nutrition metabolism prenatal exposure protein deficiency protein restriction Animals Argonaute Proteins Brain Diet, Protein-Restricted Female Karyopherins Lactation Male Maternal Nutritional Physiological Phenomena Mice MicroRNAs Pregnancy Prenatal Exposure Delayed Effects Prenatal Nutritional Physiological Phenomena Protein Deficiency |
| description |
Due to its widespread incidence, maternal malnutrition remains one of the major non-genetic factors affecting the development of newborn's brain. While all nutrients have certain influence on brain maturation, proteins appear to be the most critical for the development of neurological functions. An increasing number of studies point out that the effects of early-life nutritional inadequacy has long lasting effects on the brain and lead to permanent deficits in learning and behavior. Epigenetic mechanisms provide a potential link between the nutrition status during critical periods and changes in gene expression that may lead to disease phenotypes. Among those epigenetic mechanisms microRNAs (miRNAs) emerge as promising molecules for the link between nutrition and gene expression due to their relevance in many central nervous system functions. The objective of the current study was to evaluate the impact of perinatal protein malnutrition on the development of male and female mice offspring and to analyze the expression of the genes involved in the miRNA biogenesis pathway in different mouse brain structures. We demonstrated that early nutritional stress such as exposition to a protein-deficient diet during gestation and lactation reduced the hippocampal weight, delayed offspring's development and deregulated the expression of Xpo5 and Ago2 genes in hippocampus and hypothalamus of weanling mice. Moreover, an overall increase in mature miRNAs was consistent with the induction of Xpo5 mRNA. Altered miRNA biogenesis could modify the availability and functionality of miRNA becoming a causal factor of the adverse effects of protein malnutrition. © 2017 Elsevier B.V. |
| author |
Cánepa, Eduardo Tomás |
| author_facet |
Cánepa, Eduardo Tomás |
| author_sort |
Cánepa, Eduardo Tomás |
| title |
Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain |
| title_short |
Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain |
| title_full |
Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain |
| title_fullStr |
Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain |
| title_full_unstemmed |
Perinatal protein malnutrition alters expression of miRNA biogenesis genes Xpo5 and Ago2 in mice brain |
| title_sort |
perinatal protein malnutrition alters expression of mirna biogenesis genes xpo5 and ago2 in mice brain |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v647_n_p38_Berardino http://hdl.handle.net/20.500.12110/paper_03043940_v647_n_p38_Berardino |
| work_keys_str_mv |
AT canepaeduardotomas perinatalproteinmalnutritionaltersexpressionofmirnabiogenesisgenesxpo5andago2inmicebrain |
| _version_ |
1768542268992520192 |