Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain
N-β-Alanyldopamine (NBAD) is the primary catechol tanning agent precursor in typical brown or yellow insect cuticle. The insect integument enzyme responsible for the synthesis of NBAD was reported to be expressed solely in the epidermis, and only at the time of cuticle sclerotization. However, in th...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v368_n2_p186_Perez http://hdl.handle.net/20.500.12110/paper_03043940_v368_n2_p186_Perez |
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paper:paper_03043940_v368_n2_p186_Perez2023-06-08T15:29:31Z Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain Pérez, Martín Mariano Quesada Allué, Luis Alberto β-Alanine Biogenic amines Ceratitis capitata Medfly N-β-Alanyldopamine N-β-Alanylnorepinephrine brain amine catecholamine ligase n beta alanylcatecholamine ligase n beta alanylnorepinephrine noradrenalin unclassified drug animal tissue article catalysis central nervous system controlled study Drosophila melanogaster enzyme activity enzyme synthesis epidermis gene control gene mutation kinetics Mediterranean fruit fly nonhuman parameter priority journal protein expression protein function Animals Brain Brain Chemistry Catecholamines Cell Extracts Chromatography, High Pressure Liquid Diptera Dopamine Insect Proteins Ligases N-β-Alanyldopamine (NBAD) is the primary catechol tanning agent precursor in typical brown or yellow insect cuticle. The insect integument enzyme responsible for the synthesis of NBAD was reported to be expressed solely in the epidermis, and only at the time of cuticle sclerotization. However, in this study we demonstrate directly that the enzyme also is expressed in a constitutive manner in the neural system of insects. The requirements and kinetic parameters of the brain-associated enzyme appear similar to those of the epidermis-associated enzyme in Ceratitis capitata. The brain-associated enzyme also was able to catalyze the in vitro synthesis of N-β- alanylnorepinephrine (NBANE) and β-alanyl derivatives of other biogenic amines. A melanic mutant of C. capitata, niger, was unable to conjugate β-alanine with dopamine or other amines in either the epidermis or the brain. This result strongly supports the idea that these enzymes actually are expressed from a single gene and that differences in regulation must exist that account for the constitutive expression in the neural system. Similar results were obtained in Drosophila melanogaster and other insects. From these data, a number of questions arise about the role of β-alanyl derivatives of biogenic amines and other compounds in insect brain and similarly, in the mammalian CNS. © 2004 Elsevier Ireland Ltd. All rights reserved. Fil:Pérez, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v368_n2_p186_Perez http://hdl.handle.net/20.500.12110/paper_03043940_v368_n2_p186_Perez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Alanine Biogenic amines Ceratitis capitata Medfly N-β-Alanyldopamine N-β-Alanylnorepinephrine brain amine catecholamine ligase n beta alanylcatecholamine ligase n beta alanylnorepinephrine noradrenalin unclassified drug animal tissue article catalysis central nervous system controlled study Drosophila melanogaster enzyme activity enzyme synthesis epidermis gene control gene mutation kinetics Mediterranean fruit fly nonhuman parameter priority journal protein expression protein function Animals Brain Brain Chemistry Catecholamines Cell Extracts Chromatography, High Pressure Liquid Diptera Dopamine Insect Proteins Ligases |
| spellingShingle |
β-Alanine Biogenic amines Ceratitis capitata Medfly N-β-Alanyldopamine N-β-Alanylnorepinephrine brain amine catecholamine ligase n beta alanylcatecholamine ligase n beta alanylnorepinephrine noradrenalin unclassified drug animal tissue article catalysis central nervous system controlled study Drosophila melanogaster enzyme activity enzyme synthesis epidermis gene control gene mutation kinetics Mediterranean fruit fly nonhuman parameter priority journal protein expression protein function Animals Brain Brain Chemistry Catecholamines Cell Extracts Chromatography, High Pressure Liquid Diptera Dopamine Insect Proteins Ligases Pérez, Martín Mariano Quesada Allué, Luis Alberto Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain |
| topic_facet |
β-Alanine Biogenic amines Ceratitis capitata Medfly N-β-Alanyldopamine N-β-Alanylnorepinephrine brain amine catecholamine ligase n beta alanylcatecholamine ligase n beta alanylnorepinephrine noradrenalin unclassified drug animal tissue article catalysis central nervous system controlled study Drosophila melanogaster enzyme activity enzyme synthesis epidermis gene control gene mutation kinetics Mediterranean fruit fly nonhuman parameter priority journal protein expression protein function Animals Brain Brain Chemistry Catecholamines Cell Extracts Chromatography, High Pressure Liquid Diptera Dopamine Insect Proteins Ligases |
| description |
N-β-Alanyldopamine (NBAD) is the primary catechol tanning agent precursor in typical brown or yellow insect cuticle. The insect integument enzyme responsible for the synthesis of NBAD was reported to be expressed solely in the epidermis, and only at the time of cuticle sclerotization. However, in this study we demonstrate directly that the enzyme also is expressed in a constitutive manner in the neural system of insects. The requirements and kinetic parameters of the brain-associated enzyme appear similar to those of the epidermis-associated enzyme in Ceratitis capitata. The brain-associated enzyme also was able to catalyze the in vitro synthesis of N-β- alanylnorepinephrine (NBANE) and β-alanyl derivatives of other biogenic amines. A melanic mutant of C. capitata, niger, was unable to conjugate β-alanine with dopamine or other amines in either the epidermis or the brain. This result strongly supports the idea that these enzymes actually are expressed from a single gene and that differences in regulation must exist that account for the constitutive expression in the neural system. Similar results were obtained in Drosophila melanogaster and other insects. From these data, a number of questions arise about the role of β-alanyl derivatives of biogenic amines and other compounds in insect brain and similarly, in the mammalian CNS. © 2004 Elsevier Ireland Ltd. All rights reserved. |
| author |
Pérez, Martín Mariano Quesada Allué, Luis Alberto |
| author_facet |
Pérez, Martín Mariano Quesada Allué, Luis Alberto |
| author_sort |
Pérez, Martín Mariano |
| title |
Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain |
| title_short |
Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain |
| title_full |
Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain |
| title_fullStr |
Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain |
| title_full_unstemmed |
Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain |
| title_sort |
constitutive activity of n-β-alanyl-catecholamine ligase in insect brain |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v368_n2_p186_Perez http://hdl.handle.net/20.500.12110/paper_03043940_v368_n2_p186_Perez |
| work_keys_str_mv |
AT perezmartinmariano constitutiveactivityofnbalanylcatecholamineligaseininsectbrain AT quesadaallueluisalberto constitutiveactivityofnbalanylcatecholamineligaseininsectbrain |
| _version_ |
1768544957526704128 |