Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition
Binding of heparin to primary cultured cells of two murine mammary adenocarcinomas with low (M3) and high (MM3) lung, metastatic capacity was determined. Heparin binding was rapid, specific and saturable. MM3 cells grown for 24 h in fetal calf serum (FCS)-free medium exhibited a higher number of bin...
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1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v90_n2_p123_Bertolesix http://hdl.handle.net/20.500.12110/paper_03043835_v90_n2_p123_Bertolesix |
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paper:paper_03043835_v90_n2_p123_Bertolesix2023-06-08T15:29:20Z Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition Eijan, Ana María Growth inhibition Heparin Metastasis Receptor Tumor heparin membrane receptor tritium acetylation animal cell animal experiment animal model animal tissue article breast adenocarcinoma cell proliferation controlled study desulfurization dissociation constant female fetal calf serum growth inhibition lung metastasis mouse nonhuman priority journal Adenocarcinoma Analysis of Variance Animal Antineoplastic Agents Binding, Competitive Cell Division Female Heparin Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Metastasis Receptors, Cell Surface Support, Non-U.S. Gov't Tumor Cells, Cultured Animalia Murinae Tritium Binding of heparin to primary cultured cells of two murine mammary adenocarcinomas with low (M3) and high (MM3) lung, metastatic capacity was determined. Heparin binding was rapid, specific and saturable. MM3 cells grown for 24 h in fetal calf serum (FCS)-free medium exhibited a higher number of binding sites for 3H-heparin [(11 ± 1) × 105 sites per cell] than M3 cells [(6.9 ± 0.6) × 105 sites per cell]. However, when M3 cells were grown in the presence of 2% FCS, they showed less heparin binding sites [(3.5 ± 0.4) × 105 sites per cell]. In contrast, dissociation constants were very similar for MM3 and M3 cells grown with or without FCS (Kd = 2-4 × 10-9M). Furthermore, heparin inhibited MM3 and M3 cell growth both in the absence or presence of FCS. Competition studies showed that chemically modified heparins lacking antiproliferative effect (O-desulfated; O/N-desulfated N-acetylated and N-desulfated heparins) were not able to inhibit 3H-heparin binding. N-desulfated N-acetylated heparin, which had partial antiproliferative effect, partially inhibited 3H-heparin binding, while heparin with a high antiproliferative activity inhibited more than 90% 3H-heparin binding. The antiproliferative effect of heparin and chemically modified heparins seems to be related to their binding ability to the cell membrane. © 1995. Fil:Eiján, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v90_n2_p123_Bertolesix http://hdl.handle.net/20.500.12110/paper_03043835_v90_n2_p123_Bertolesix |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Growth inhibition Heparin Metastasis Receptor Tumor heparin membrane receptor tritium acetylation animal cell animal experiment animal model animal tissue article breast adenocarcinoma cell proliferation controlled study desulfurization dissociation constant female fetal calf serum growth inhibition lung metastasis mouse nonhuman priority journal Adenocarcinoma Analysis of Variance Animal Antineoplastic Agents Binding, Competitive Cell Division Female Heparin Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Metastasis Receptors, Cell Surface Support, Non-U.S. Gov't Tumor Cells, Cultured Animalia Murinae Tritium |
| spellingShingle |
Growth inhibition Heparin Metastasis Receptor Tumor heparin membrane receptor tritium acetylation animal cell animal experiment animal model animal tissue article breast adenocarcinoma cell proliferation controlled study desulfurization dissociation constant female fetal calf serum growth inhibition lung metastasis mouse nonhuman priority journal Adenocarcinoma Analysis of Variance Animal Antineoplastic Agents Binding, Competitive Cell Division Female Heparin Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Metastasis Receptors, Cell Surface Support, Non-U.S. Gov't Tumor Cells, Cultured Animalia Murinae Tritium Eijan, Ana María Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| topic_facet |
Growth inhibition Heparin Metastasis Receptor Tumor heparin membrane receptor tritium acetylation animal cell animal experiment animal model animal tissue article breast adenocarcinoma cell proliferation controlled study desulfurization dissociation constant female fetal calf serum growth inhibition lung metastasis mouse nonhuman priority journal Adenocarcinoma Analysis of Variance Animal Antineoplastic Agents Binding, Competitive Cell Division Female Heparin Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Metastasis Receptors, Cell Surface Support, Non-U.S. Gov't Tumor Cells, Cultured Animalia Murinae Tritium |
| description |
Binding of heparin to primary cultured cells of two murine mammary adenocarcinomas with low (M3) and high (MM3) lung, metastatic capacity was determined. Heparin binding was rapid, specific and saturable. MM3 cells grown for 24 h in fetal calf serum (FCS)-free medium exhibited a higher number of binding sites for 3H-heparin [(11 ± 1) × 105 sites per cell] than M3 cells [(6.9 ± 0.6) × 105 sites per cell]. However, when M3 cells were grown in the presence of 2% FCS, they showed less heparin binding sites [(3.5 ± 0.4) × 105 sites per cell]. In contrast, dissociation constants were very similar for MM3 and M3 cells grown with or without FCS (Kd = 2-4 × 10-9M). Furthermore, heparin inhibited MM3 and M3 cell growth both in the absence or presence of FCS. Competition studies showed that chemically modified heparins lacking antiproliferative effect (O-desulfated; O/N-desulfated N-acetylated and N-desulfated heparins) were not able to inhibit 3H-heparin binding. N-desulfated N-acetylated heparin, which had partial antiproliferative effect, partially inhibited 3H-heparin binding, while heparin with a high antiproliferative activity inhibited more than 90% 3H-heparin binding. The antiproliferative effect of heparin and chemically modified heparins seems to be related to their binding ability to the cell membrane. © 1995. |
| author |
Eijan, Ana María |
| author_facet |
Eijan, Ana María |
| author_sort |
Eijan, Ana María |
| title |
Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| title_short |
Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| title_full |
Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| title_fullStr |
Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| title_full_unstemmed |
Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| title_sort |
heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v90_n2_p123_Bertolesix http://hdl.handle.net/20.500.12110/paper_03043835_v90_n2_p123_Bertolesix |
| work_keys_str_mv |
AT eijananamaria heparinreceptorsintwomurinemammaryadenocarcinomaswithdifferentmetastaticabilityrelationshipwithgrowthinhibition |
| _version_ |
1768542738476695552 |