Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells
Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophobl...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v443_n_p146_Vota http://hdl.handle.net/20.500.12110/paper_03037207_v443_n_p146_Vota |
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paper:paper_03037207_v443_n_p146_Vota2023-06-08T15:29:13Z Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells Maternal–placental interface Phagocytosis Progesterone Trophoblast-derived cells Vasoactive intestinal polypeptide glyceraldehyde 3 phosphate dehydrogenase interferon regulatory factor 5 interleukin 1 interleukin 1beta interleukin 6 kruppel like factor 4 nitrite peroxisome proliferator activated receptor gamma progesterone transforming growth factor beta transforming growth factor beta1 vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 antiinflammatory agent progesterone transcription factor vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 antiinflammatory activity Article controlled study erythrocyte gene overexpression human human cell phagocytosis protein expression protein protein interaction trophoblast cell line cytology drug effects metabolism Anti-Inflammatory Agents Cell Line Humans Phagocytosis Progesterone Receptors, Vasoactive Intestinal Peptide, Type II Transcription Factors Trophoblasts Vasoactive Intestinal Peptide Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophoblast cell function, focus on the phagocytic ability and the immune homeostasis maintenance. P4 enhanced the phagocytosis in trophoblast-derived cells quantified by the engulfment of latex-beads or eryptotic erythrocytes. P4 and VIP modulated the balance of anti/pro-inflammatory mediators, increasing TGF-β expression, with no changes in IL-1, IL-6, or nitrites production. This modulation was accompained by transcription factor expression changes that could turn on tolerogenic programs represented by increased PPAR-γ and decreased IRF-5 expression. Finally, P4 stimulated VPAC2 expression in trophoblast cells and VPAC2 over-expression enhanced phagocytosis mimicking P4-effect. Therefore, P4 and VIP network enhances the phagocytic ability of trophoblast-derived cells, through a mechanism involving VPAC2 accompained with an anti-inflammatory context. © 2017 Elsevier B.V. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v443_n_p146_Vota http://hdl.handle.net/20.500.12110/paper_03037207_v443_n_p146_Vota |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Maternal–placental interface Phagocytosis Progesterone Trophoblast-derived cells Vasoactive intestinal polypeptide glyceraldehyde 3 phosphate dehydrogenase interferon regulatory factor 5 interleukin 1 interleukin 1beta interleukin 6 kruppel like factor 4 nitrite peroxisome proliferator activated receptor gamma progesterone transforming growth factor beta transforming growth factor beta1 vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 antiinflammatory agent progesterone transcription factor vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 antiinflammatory activity Article controlled study erythrocyte gene overexpression human human cell phagocytosis protein expression protein protein interaction trophoblast cell line cytology drug effects metabolism Anti-Inflammatory Agents Cell Line Humans Phagocytosis Progesterone Receptors, Vasoactive Intestinal Peptide, Type II Transcription Factors Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
Maternal–placental interface Phagocytosis Progesterone Trophoblast-derived cells Vasoactive intestinal polypeptide glyceraldehyde 3 phosphate dehydrogenase interferon regulatory factor 5 interleukin 1 interleukin 1beta interleukin 6 kruppel like factor 4 nitrite peroxisome proliferator activated receptor gamma progesterone transforming growth factor beta transforming growth factor beta1 vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 antiinflammatory agent progesterone transcription factor vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 antiinflammatory activity Article controlled study erythrocyte gene overexpression human human cell phagocytosis protein expression protein protein interaction trophoblast cell line cytology drug effects metabolism Anti-Inflammatory Agents Cell Line Humans Phagocytosis Progesterone Receptors, Vasoactive Intestinal Peptide, Type II Transcription Factors Trophoblasts Vasoactive Intestinal Peptide Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| topic_facet |
Maternal–placental interface Phagocytosis Progesterone Trophoblast-derived cells Vasoactive intestinal polypeptide glyceraldehyde 3 phosphate dehydrogenase interferon regulatory factor 5 interleukin 1 interleukin 1beta interleukin 6 kruppel like factor 4 nitrite peroxisome proliferator activated receptor gamma progesterone transforming growth factor beta transforming growth factor beta1 vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 antiinflammatory agent progesterone transcription factor vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 antiinflammatory activity Article controlled study erythrocyte gene overexpression human human cell phagocytosis protein expression protein protein interaction trophoblast cell line cytology drug effects metabolism Anti-Inflammatory Agents Cell Line Humans Phagocytosis Progesterone Receptors, Vasoactive Intestinal Peptide, Type II Transcription Factors Trophoblasts Vasoactive Intestinal Peptide |
| description |
Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophoblast cell function, focus on the phagocytic ability and the immune homeostasis maintenance. P4 enhanced the phagocytosis in trophoblast-derived cells quantified by the engulfment of latex-beads or eryptotic erythrocytes. P4 and VIP modulated the balance of anti/pro-inflammatory mediators, increasing TGF-β expression, with no changes in IL-1, IL-6, or nitrites production. This modulation was accompained by transcription factor expression changes that could turn on tolerogenic programs represented by increased PPAR-γ and decreased IRF-5 expression. Finally, P4 stimulated VPAC2 expression in trophoblast cells and VPAC2 over-expression enhanced phagocytosis mimicking P4-effect. Therefore, P4 and VIP network enhances the phagocytic ability of trophoblast-derived cells, through a mechanism involving VPAC2 accompained with an anti-inflammatory context. © 2017 Elsevier B.V. |
| title |
Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| title_short |
Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| title_full |
Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| title_fullStr |
Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| title_full_unstemmed |
Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| title_sort |
progesterone and vip cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v443_n_p146_Vota http://hdl.handle.net/20.500.12110/paper_03037207_v443_n_p146_Vota |
| _version_ |
1768542645266677760 |