Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens
Transgenic female mice overexpressing the α- and β. - subunits of human chorionic gonadotropin (hCGαβ+) exhibited precocious puberty, as evidenced by early vaginal opening. Chronically elevated hCG in 21-day-old hCGαβ+ females stimulated gonadal androgen production, which exerted negative feedback o...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v390_n1-2_p102_Gonzalez http://hdl.handle.net/20.500.12110/paper_03037207_v390_n1-2_p102_Gonzalez |
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paper:paper_03037207_v390_n1-2_p102_Gonzalez2023-06-08T15:29:11Z Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens Aromatase Human chorionic gonadotropin Hypothalamic-pituitary-gonadal axis Precocious puberty Transgenic mice androgen aromatase chorionic gonadotropin alpha subunit chorionic gonadotropin beta subunit flutamide follitropin gonadorelin luteinizing hormone antiandrogen aromatase chorionic gonadotropin estradiol flutamide follitropin gonadorelin testosterone androgen synthesis animal cell animal experiment animal model animal tissue aromatization article controlled study ex vivo study female fetus gene expression gonadotropin release hormone determination hypothalamus hypophysis gonad system in vitro study mouse negative feedback nonhuman organ weight precocious puberty preoptic area priority journal sex difference transgenic mouse wild type animal blood cell culture human hypophysis hypothalamus metabolism pathophysiology physiology Puberty, Precocious secretion (process) vagina Androgen Antagonists Animals Aromatase Cells, Cultured Chorionic Gonadotropin Estradiol Female Flutamide Follicle Stimulating Hormone Gene Expression Gonadotropin-Releasing Hormone Humans Hypothalamus Mice, Transgenic Pituitary Gland Puberty, Precocious Testosterone Vagina Transgenic female mice overexpressing the α- and β. - subunits of human chorionic gonadotropin (hCGαβ+) exhibited precocious puberty, as evidenced by early vaginal opening. Chronically elevated hCG in 21-day-old hCGαβ+ females stimulated gonadal androgen production, which exerted negative feedback over the endogenous gonadotropin synthesis, and activated the hypothalamic GnRH pulsatility and gene expression. Transgenic females also exhibited elevated hypothalamic aromatization in the preoptic area (POA), which is the sexually-differentiated area that controls the LH surge in adulthood. Ovariectomy at 14. days of age was unable to rescue this phenotype. However, the blockade of androgen action by flutamide from postnatal day 6 onwards reduced the aromatase levels in the POA of hCGαβ+ females. Our results suggest that early exposure of females to androgen action during a critical period between postnatal days 6-14 induces sex-specific organizational changes of the brain, which affect the aromatase expression in the POA at the onset of precocious puberty. © 2014 Elsevier Ireland Ltd. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v390_n1-2_p102_Gonzalez http://hdl.handle.net/20.500.12110/paper_03037207_v390_n1-2_p102_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aromatase Human chorionic gonadotropin Hypothalamic-pituitary-gonadal axis Precocious puberty Transgenic mice androgen aromatase chorionic gonadotropin alpha subunit chorionic gonadotropin beta subunit flutamide follitropin gonadorelin luteinizing hormone antiandrogen aromatase chorionic gonadotropin estradiol flutamide follitropin gonadorelin testosterone androgen synthesis animal cell animal experiment animal model animal tissue aromatization article controlled study ex vivo study female fetus gene expression gonadotropin release hormone determination hypothalamus hypophysis gonad system in vitro study mouse negative feedback nonhuman organ weight precocious puberty preoptic area priority journal sex difference transgenic mouse wild type animal blood cell culture human hypophysis hypothalamus metabolism pathophysiology physiology Puberty, Precocious secretion (process) vagina Androgen Antagonists Animals Aromatase Cells, Cultured Chorionic Gonadotropin Estradiol Female Flutamide Follicle Stimulating Hormone Gene Expression Gonadotropin-Releasing Hormone Humans Hypothalamus Mice, Transgenic Pituitary Gland Puberty, Precocious Testosterone Vagina |
| spellingShingle |
Aromatase Human chorionic gonadotropin Hypothalamic-pituitary-gonadal axis Precocious puberty Transgenic mice androgen aromatase chorionic gonadotropin alpha subunit chorionic gonadotropin beta subunit flutamide follitropin gonadorelin luteinizing hormone antiandrogen aromatase chorionic gonadotropin estradiol flutamide follitropin gonadorelin testosterone androgen synthesis animal cell animal experiment animal model animal tissue aromatization article controlled study ex vivo study female fetus gene expression gonadotropin release hormone determination hypothalamus hypophysis gonad system in vitro study mouse negative feedback nonhuman organ weight precocious puberty preoptic area priority journal sex difference transgenic mouse wild type animal blood cell culture human hypophysis hypothalamus metabolism pathophysiology physiology Puberty, Precocious secretion (process) vagina Androgen Antagonists Animals Aromatase Cells, Cultured Chorionic Gonadotropin Estradiol Female Flutamide Follicle Stimulating Hormone Gene Expression Gonadotropin-Releasing Hormone Humans Hypothalamus Mice, Transgenic Pituitary Gland Puberty, Precocious Testosterone Vagina Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens |
| topic_facet |
Aromatase Human chorionic gonadotropin Hypothalamic-pituitary-gonadal axis Precocious puberty Transgenic mice androgen aromatase chorionic gonadotropin alpha subunit chorionic gonadotropin beta subunit flutamide follitropin gonadorelin luteinizing hormone antiandrogen aromatase chorionic gonadotropin estradiol flutamide follitropin gonadorelin testosterone androgen synthesis animal cell animal experiment animal model animal tissue aromatization article controlled study ex vivo study female fetus gene expression gonadotropin release hormone determination hypothalamus hypophysis gonad system in vitro study mouse negative feedback nonhuman organ weight precocious puberty preoptic area priority journal sex difference transgenic mouse wild type animal blood cell culture human hypophysis hypothalamus metabolism pathophysiology physiology Puberty, Precocious secretion (process) vagina Androgen Antagonists Animals Aromatase Cells, Cultured Chorionic Gonadotropin Estradiol Female Flutamide Follicle Stimulating Hormone Gene Expression Gonadotropin-Releasing Hormone Humans Hypothalamus Mice, Transgenic Pituitary Gland Puberty, Precocious Testosterone Vagina |
| description |
Transgenic female mice overexpressing the α- and β. - subunits of human chorionic gonadotropin (hCGαβ+) exhibited precocious puberty, as evidenced by early vaginal opening. Chronically elevated hCG in 21-day-old hCGαβ+ females stimulated gonadal androgen production, which exerted negative feedback over the endogenous gonadotropin synthesis, and activated the hypothalamic GnRH pulsatility and gene expression. Transgenic females also exhibited elevated hypothalamic aromatization in the preoptic area (POA), which is the sexually-differentiated area that controls the LH surge in adulthood. Ovariectomy at 14. days of age was unable to rescue this phenotype. However, the blockade of androgen action by flutamide from postnatal day 6 onwards reduced the aromatase levels in the POA of hCGαβ+ females. Our results suggest that early exposure of females to androgen action during a critical period between postnatal days 6-14 induces sex-specific organizational changes of the brain, which affect the aromatase expression in the POA at the onset of precocious puberty. © 2014 Elsevier Ireland Ltd. |
| title |
Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens |
| title_short |
Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens |
| title_full |
Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens |
| title_fullStr |
Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens |
| title_full_unstemmed |
Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens |
| title_sort |
elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: effect of androgens |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v390_n1-2_p102_Gonzalez http://hdl.handle.net/20.500.12110/paper_03037207_v390_n1-2_p102_Gonzalez |
| _version_ |
1768544409381502976 |