Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells
The antagonism exerted by melatonin on the glucocorticoid response has been well established, being strongly dependent on the cellular context. Previously, we found that melatonin inhibits glucocorticoid receptor (GR) dissociation from the chaperone hetero-complex and nuclear translocation on mouse...
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2012
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paper:paper_03037207_v349_n2_p214_Presman2023-06-08T15:29:09Z Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells Presman, Diego Martín Levi, Valeria Pignataro, Omar Pedro Pecci, Adali Antagonism Glucocorticoids Luzindole Melatonin TIF2 cyclic AMP glucocorticoid receptor luzindole melatonin nuclear receptor coactivator 2 cyclic AMP dexamethasone glucocorticoid glucocorticoid receptor melatonin melatonin receptor nuclear receptor coactivator 2 tryptamine derivative animal cell article cellular distribution concentration response controlled study dimerization down regulation enzyme inhibition fluorescence microscopy gene expression regulation hamster homodimerization immunoprecipitation kidney cell culture nerve cell newborn nonhuman nuclear import oligomerization priority journal promoter region protein DNA interaction protein domain protein expression protein localization protein protein interaction thymocyte transcription initiation active transport animal cell line drug antagonism drug effect gene expression genetics metabolism Mouse mammary tumor oncovirus signal transduction Active Transport, Cell Nucleus Animals Cell Line Cricetinae Cyclic AMP Dexamethasone Dimerization Gene Expression Glucocorticoids Immunoprecipitation Mammary Tumor Virus, Mouse Melatonin Microscopy, Fluorescence Nuclear Receptor Coactivator 2 Receptors, Glucocorticoid Receptors, Melatonin Signal Transduction Tryptamines The antagonism exerted by melatonin on the glucocorticoid response has been well established, being strongly dependent on the cellular context. Previously, we found that melatonin inhibits glucocorticoid receptor (GR) dissociation from the chaperone hetero-complex and nuclear translocation on mouse thymocytes. Here, by performing confocal fluorescence microscopy and the Number and Brightness assay we show that in newborn hamster kidney cells (BHK21) melatonin neither affects GR nuclear translocation nor GR homodimerization. Instead, co-immunoprecipitation studies suggest that physiological concentrations of melatonin impair GR interaction with the transcriptional intermediary factor 2 (TIF2). This melatonin effect was not blocked by the MT 1/MT 2 receptor antagonist luzindole. Curiously, luzindole behaved as an antiglucocorticoid per se by impairing the glucocorticoid-dependent MMTV-driven gene expression affecting neither GR translocation nor GR-TIF2 interaction. © 2011 Elsevier Ireland Ltd. Fil:Presman, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v349_n2_p214_Presman http://hdl.handle.net/20.500.12110/paper_03037207_v349_n2_p214_Presman |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antagonism Glucocorticoids Luzindole Melatonin TIF2 cyclic AMP glucocorticoid receptor luzindole melatonin nuclear receptor coactivator 2 cyclic AMP dexamethasone glucocorticoid glucocorticoid receptor melatonin melatonin receptor nuclear receptor coactivator 2 tryptamine derivative animal cell article cellular distribution concentration response controlled study dimerization down regulation enzyme inhibition fluorescence microscopy gene expression regulation hamster homodimerization immunoprecipitation kidney cell culture nerve cell newborn nonhuman nuclear import oligomerization priority journal promoter region protein DNA interaction protein domain protein expression protein localization protein protein interaction thymocyte transcription initiation active transport animal cell line drug antagonism drug effect gene expression genetics metabolism Mouse mammary tumor oncovirus signal transduction Active Transport, Cell Nucleus Animals Cell Line Cricetinae Cyclic AMP Dexamethasone Dimerization Gene Expression Glucocorticoids Immunoprecipitation Mammary Tumor Virus, Mouse Melatonin Microscopy, Fluorescence Nuclear Receptor Coactivator 2 Receptors, Glucocorticoid Receptors, Melatonin Signal Transduction Tryptamines |
| spellingShingle |
Antagonism Glucocorticoids Luzindole Melatonin TIF2 cyclic AMP glucocorticoid receptor luzindole melatonin nuclear receptor coactivator 2 cyclic AMP dexamethasone glucocorticoid glucocorticoid receptor melatonin melatonin receptor nuclear receptor coactivator 2 tryptamine derivative animal cell article cellular distribution concentration response controlled study dimerization down regulation enzyme inhibition fluorescence microscopy gene expression regulation hamster homodimerization immunoprecipitation kidney cell culture nerve cell newborn nonhuman nuclear import oligomerization priority journal promoter region protein DNA interaction protein domain protein expression protein localization protein protein interaction thymocyte transcription initiation active transport animal cell line drug antagonism drug effect gene expression genetics metabolism Mouse mammary tumor oncovirus signal transduction Active Transport, Cell Nucleus Animals Cell Line Cricetinae Cyclic AMP Dexamethasone Dimerization Gene Expression Glucocorticoids Immunoprecipitation Mammary Tumor Virus, Mouse Melatonin Microscopy, Fluorescence Nuclear Receptor Coactivator 2 Receptors, Glucocorticoid Receptors, Melatonin Signal Transduction Tryptamines Presman, Diego Martín Levi, Valeria Pignataro, Omar Pedro Pecci, Adali Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells |
| topic_facet |
Antagonism Glucocorticoids Luzindole Melatonin TIF2 cyclic AMP glucocorticoid receptor luzindole melatonin nuclear receptor coactivator 2 cyclic AMP dexamethasone glucocorticoid glucocorticoid receptor melatonin melatonin receptor nuclear receptor coactivator 2 tryptamine derivative animal cell article cellular distribution concentration response controlled study dimerization down regulation enzyme inhibition fluorescence microscopy gene expression regulation hamster homodimerization immunoprecipitation kidney cell culture nerve cell newborn nonhuman nuclear import oligomerization priority journal promoter region protein DNA interaction protein domain protein expression protein localization protein protein interaction thymocyte transcription initiation active transport animal cell line drug antagonism drug effect gene expression genetics metabolism Mouse mammary tumor oncovirus signal transduction Active Transport, Cell Nucleus Animals Cell Line Cricetinae Cyclic AMP Dexamethasone Dimerization Gene Expression Glucocorticoids Immunoprecipitation Mammary Tumor Virus, Mouse Melatonin Microscopy, Fluorescence Nuclear Receptor Coactivator 2 Receptors, Glucocorticoid Receptors, Melatonin Signal Transduction Tryptamines |
| description |
The antagonism exerted by melatonin on the glucocorticoid response has been well established, being strongly dependent on the cellular context. Previously, we found that melatonin inhibits glucocorticoid receptor (GR) dissociation from the chaperone hetero-complex and nuclear translocation on mouse thymocytes. Here, by performing confocal fluorescence microscopy and the Number and Brightness assay we show that in newborn hamster kidney cells (BHK21) melatonin neither affects GR nuclear translocation nor GR homodimerization. Instead, co-immunoprecipitation studies suggest that physiological concentrations of melatonin impair GR interaction with the transcriptional intermediary factor 2 (TIF2). This melatonin effect was not blocked by the MT 1/MT 2 receptor antagonist luzindole. Curiously, luzindole behaved as an antiglucocorticoid per se by impairing the glucocorticoid-dependent MMTV-driven gene expression affecting neither GR translocation nor GR-TIF2 interaction. © 2011 Elsevier Ireland Ltd. |
| author |
Presman, Diego Martín Levi, Valeria Pignataro, Omar Pedro Pecci, Adali |
| author_facet |
Presman, Diego Martín Levi, Valeria Pignataro, Omar Pedro Pecci, Adali |
| author_sort |
Presman, Diego Martín |
| title |
Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells |
| title_short |
Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells |
| title_full |
Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells |
| title_fullStr |
Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells |
| title_full_unstemmed |
Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells |
| title_sort |
melatonin inhibits glucocorticoid-dependent gr-tif2 interaction in newborn hamster kidney (bhk) cells |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v349_n2_p214_Presman http://hdl.handle.net/20.500.12110/paper_03037207_v349_n2_p214_Presman |
| work_keys_str_mv |
AT presmandiegomartin melatonininhibitsglucocorticoiddependentgrtif2interactioninnewbornhamsterkidneybhkcells AT levivaleria melatonininhibitsglucocorticoiddependentgrtif2interactioninnewbornhamsterkidneybhkcells AT pignataroomarpedro melatonininhibitsglucocorticoiddependentgrtif2interactioninnewbornhamsterkidneybhkcells AT pecciadali melatonininhibitsglucocorticoiddependentgrtif2interactioninnewbornhamsterkidneybhkcells |
| _version_ |
1768543368990687232 |