PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). Howe...
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paper:paper_03037207_v298_n1-2_p42_Martini2023-06-08T15:29:06Z PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation Martini, Claudia Noemí Vila, María del Carmen 3T3-L1 fibroblast Adipogenesis cAMP Insulin Methylisobutylxanthine (MIX) Mitotic clonal expansion PKA 8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate bucladesine cyclic AMP cyclic AMP dependent protein kinase n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide unclassified drug adipocyte animal cell article catalysis cell differentiation fibroblast genetic transfection mouse nonhuman priority journal protein expression 1-Methyl-3-isobutylxanthine 3T3-L1 Cells Adipogenesis Animals Cell Differentiation Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dexamethasone Fibroblasts Insulin Isoquinolines Mice Mitosis Protein Kinase Inhibitors Signal Transduction Sulfonamides Theophylline Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). However, PKA-independent cAMP signaling has also been described. In this paper, it was found that H89, an inhibitor of PKA, was able to block MCE but not differentiation of 3T3-L1 fibroblast. Consistently, MCE did not occur in the absence of MIX in the differentiation mixture but was recovered by overexpression of a catalytic subunit of PKA. In addition, the transfection of 3T3-L1 fibroblast with a dominant-negative mutant of PKA inhibited MCE. On the other hand, differentiation of 3T3-L1 fibroblast to adipocytes did not occur when MIX was not present in the differentiation mixture and it could not be recovered by overexpression of a catalytic subunit of PKA. Differentiation was restored by addition of either dibutyryl-cAMP (db-cAMP) or 8 CPT-2 Me-cAMP. The latter activates cAMP-EPAC but not PKA signaling. These results indicate that cAMP-PKA-independent signaling, is required for 3T3-L1 fibroblasts differentiation to adipocytes and MIX signaling through cAMP-PKA is necessary for MCE, although MCE is not essential for adipogenesis. © 2008 Elsevier Ireland Ltd. All rights reserved. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.d.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v298_n1-2_p42_Martini http://hdl.handle.net/20.500.12110/paper_03037207_v298_n1-2_p42_Martini |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
3T3-L1 fibroblast Adipogenesis cAMP Insulin Methylisobutylxanthine (MIX) Mitotic clonal expansion PKA 8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate bucladesine cyclic AMP cyclic AMP dependent protein kinase n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide unclassified drug adipocyte animal cell article catalysis cell differentiation fibroblast genetic transfection mouse nonhuman priority journal protein expression 1-Methyl-3-isobutylxanthine 3T3-L1 Cells Adipogenesis Animals Cell Differentiation Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dexamethasone Fibroblasts Insulin Isoquinolines Mice Mitosis Protein Kinase Inhibitors Signal Transduction Sulfonamides Theophylline |
spellingShingle |
3T3-L1 fibroblast Adipogenesis cAMP Insulin Methylisobutylxanthine (MIX) Mitotic clonal expansion PKA 8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate bucladesine cyclic AMP cyclic AMP dependent protein kinase n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide unclassified drug adipocyte animal cell article catalysis cell differentiation fibroblast genetic transfection mouse nonhuman priority journal protein expression 1-Methyl-3-isobutylxanthine 3T3-L1 Cells Adipogenesis Animals Cell Differentiation Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dexamethasone Fibroblasts Insulin Isoquinolines Mice Mitosis Protein Kinase Inhibitors Signal Transduction Sulfonamides Theophylline Martini, Claudia Noemí Vila, María del Carmen PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
topic_facet |
3T3-L1 fibroblast Adipogenesis cAMP Insulin Methylisobutylxanthine (MIX) Mitotic clonal expansion PKA 8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate bucladesine cyclic AMP cyclic AMP dependent protein kinase n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide unclassified drug adipocyte animal cell article catalysis cell differentiation fibroblast genetic transfection mouse nonhuman priority journal protein expression 1-Methyl-3-isobutylxanthine 3T3-L1 Cells Adipogenesis Animals Cell Differentiation Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dexamethasone Fibroblasts Insulin Isoquinolines Mice Mitosis Protein Kinase Inhibitors Signal Transduction Sulfonamides Theophylline |
description |
Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). However, PKA-independent cAMP signaling has also been described. In this paper, it was found that H89, an inhibitor of PKA, was able to block MCE but not differentiation of 3T3-L1 fibroblast. Consistently, MCE did not occur in the absence of MIX in the differentiation mixture but was recovered by overexpression of a catalytic subunit of PKA. In addition, the transfection of 3T3-L1 fibroblast with a dominant-negative mutant of PKA inhibited MCE. On the other hand, differentiation of 3T3-L1 fibroblast to adipocytes did not occur when MIX was not present in the differentiation mixture and it could not be recovered by overexpression of a catalytic subunit of PKA. Differentiation was restored by addition of either dibutyryl-cAMP (db-cAMP) or 8 CPT-2 Me-cAMP. The latter activates cAMP-EPAC but not PKA signaling. These results indicate that cAMP-PKA-independent signaling, is required for 3T3-L1 fibroblasts differentiation to adipocytes and MIX signaling through cAMP-PKA is necessary for MCE, although MCE is not essential for adipogenesis. © 2008 Elsevier Ireland Ltd. All rights reserved. |
author |
Martini, Claudia Noemí Vila, María del Carmen |
author_facet |
Martini, Claudia Noemí Vila, María del Carmen |
author_sort |
Martini, Claudia Noemí |
title |
PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
title_short |
PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
title_full |
PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
title_fullStr |
PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
title_full_unstemmed |
PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
title_sort |
pka-dependent and independent camp signaling in 3t3-l1 fibroblasts differentiation |
publishDate |
2009 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v298_n1-2_p42_Martini http://hdl.handle.net/20.500.12110/paper_03037207_v298_n1-2_p42_Martini |
work_keys_str_mv |
AT martiniclaudianoemi pkadependentandindependentcampsignalingin3t3l1fibroblastsdifferentiation AT vilamariadelcarmen pkadependentandindependentcampsignalingin3t3l1fibroblastsdifferentiation |
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