PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation

Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). Howe...

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Autores principales: Martini, Claudia Noemí, Vila, María del Carmen
Publicado: 2009
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PKA
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v298_n1-2_p42_Martini
http://hdl.handle.net/20.500.12110/paper_03037207_v298_n1-2_p42_Martini
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spelling paper:paper_03037207_v298_n1-2_p42_Martini2023-06-08T15:29:06Z PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation Martini, Claudia Noemí Vila, María del Carmen 3T3-L1 fibroblast Adipogenesis cAMP Insulin Methylisobutylxanthine (MIX) Mitotic clonal expansion PKA 8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate bucladesine cyclic AMP cyclic AMP dependent protein kinase n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide unclassified drug adipocyte animal cell article catalysis cell differentiation fibroblast genetic transfection mouse nonhuman priority journal protein expression 1-Methyl-3-isobutylxanthine 3T3-L1 Cells Adipogenesis Animals Cell Differentiation Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dexamethasone Fibroblasts Insulin Isoquinolines Mice Mitosis Protein Kinase Inhibitors Signal Transduction Sulfonamides Theophylline Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). However, PKA-independent cAMP signaling has also been described. In this paper, it was found that H89, an inhibitor of PKA, was able to block MCE but not differentiation of 3T3-L1 fibroblast. Consistently, MCE did not occur in the absence of MIX in the differentiation mixture but was recovered by overexpression of a catalytic subunit of PKA. In addition, the transfection of 3T3-L1 fibroblast with a dominant-negative mutant of PKA inhibited MCE. On the other hand, differentiation of 3T3-L1 fibroblast to adipocytes did not occur when MIX was not present in the differentiation mixture and it could not be recovered by overexpression of a catalytic subunit of PKA. Differentiation was restored by addition of either dibutyryl-cAMP (db-cAMP) or 8 CPT-2 Me-cAMP. The latter activates cAMP-EPAC but not PKA signaling. These results indicate that cAMP-PKA-independent signaling, is required for 3T3-L1 fibroblasts differentiation to adipocytes and MIX signaling through cAMP-PKA is necessary for MCE, although MCE is not essential for adipogenesis. © 2008 Elsevier Ireland Ltd. All rights reserved. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.d.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v298_n1-2_p42_Martini http://hdl.handle.net/20.500.12110/paper_03037207_v298_n1-2_p42_Martini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 3T3-L1 fibroblast
Adipogenesis
cAMP
Insulin
Methylisobutylxanthine (MIX)
Mitotic clonal expansion
PKA
8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate
bucladesine
cyclic AMP
cyclic AMP dependent protein kinase
n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide
unclassified drug
adipocyte
animal cell
article
catalysis
cell differentiation
fibroblast
genetic transfection
mouse
nonhuman
priority journal
protein expression
1-Methyl-3-isobutylxanthine
3T3-L1 Cells
Adipogenesis
Animals
Cell Differentiation
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases
Dexamethasone
Fibroblasts
Insulin
Isoquinolines
Mice
Mitosis
Protein Kinase Inhibitors
Signal Transduction
Sulfonamides
Theophylline
spellingShingle 3T3-L1 fibroblast
Adipogenesis
cAMP
Insulin
Methylisobutylxanthine (MIX)
Mitotic clonal expansion
PKA
8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate
bucladesine
cyclic AMP
cyclic AMP dependent protein kinase
n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide
unclassified drug
adipocyte
animal cell
article
catalysis
cell differentiation
fibroblast
genetic transfection
mouse
nonhuman
priority journal
protein expression
1-Methyl-3-isobutylxanthine
3T3-L1 Cells
Adipogenesis
Animals
Cell Differentiation
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases
Dexamethasone
Fibroblasts
Insulin
Isoquinolines
Mice
Mitosis
Protein Kinase Inhibitors
Signal Transduction
Sulfonamides
Theophylline
Martini, Claudia Noemí
Vila, María del Carmen
PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
topic_facet 3T3-L1 fibroblast
Adipogenesis
cAMP
Insulin
Methylisobutylxanthine (MIX)
Mitotic clonal expansion
PKA
8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate
bucladesine
cyclic AMP
cyclic AMP dependent protein kinase
n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide
unclassified drug
adipocyte
animal cell
article
catalysis
cell differentiation
fibroblast
genetic transfection
mouse
nonhuman
priority journal
protein expression
1-Methyl-3-isobutylxanthine
3T3-L1 Cells
Adipogenesis
Animals
Cell Differentiation
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases
Dexamethasone
Fibroblasts
Insulin
Isoquinolines
Mice
Mitosis
Protein Kinase Inhibitors
Signal Transduction
Sulfonamides
Theophylline
description Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). However, PKA-independent cAMP signaling has also been described. In this paper, it was found that H89, an inhibitor of PKA, was able to block MCE but not differentiation of 3T3-L1 fibroblast. Consistently, MCE did not occur in the absence of MIX in the differentiation mixture but was recovered by overexpression of a catalytic subunit of PKA. In addition, the transfection of 3T3-L1 fibroblast with a dominant-negative mutant of PKA inhibited MCE. On the other hand, differentiation of 3T3-L1 fibroblast to adipocytes did not occur when MIX was not present in the differentiation mixture and it could not be recovered by overexpression of a catalytic subunit of PKA. Differentiation was restored by addition of either dibutyryl-cAMP (db-cAMP) or 8 CPT-2 Me-cAMP. The latter activates cAMP-EPAC but not PKA signaling. These results indicate that cAMP-PKA-independent signaling, is required for 3T3-L1 fibroblasts differentiation to adipocytes and MIX signaling through cAMP-PKA is necessary for MCE, although MCE is not essential for adipogenesis. © 2008 Elsevier Ireland Ltd. All rights reserved.
author Martini, Claudia Noemí
Vila, María del Carmen
author_facet Martini, Claudia Noemí
Vila, María del Carmen
author_sort Martini, Claudia Noemí
title PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
title_short PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
title_full PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
title_fullStr PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
title_full_unstemmed PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
title_sort pka-dependent and independent camp signaling in 3t3-l1 fibroblasts differentiation
publishDate 2009
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v298_n1-2_p42_Martini
http://hdl.handle.net/20.500.12110/paper_03037207_v298_n1-2_p42_Martini
work_keys_str_mv AT martiniclaudianoemi pkadependentandindependentcampsignalingin3t3l1fibroblastsdifferentiation
AT vilamariadelcarmen pkadependentandindependentcampsignalingin3t3l1fibroblastsdifferentiation
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