Activin and follistatin in rat mammary gland

Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types...

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Detalles Bibliográficos
Autores principales:	Bussmann, Ursula A., Lanuza, Guillermo
Publicado:	2004
Materias:	Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v221_n1-2_p9_Bussmann http://hdl.handle.net/20.500.12110/paper_03037207_v221_n1-2_p9_Bussmann
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03037207_v221_n1-2_p9_Bussmann
record_format	dspace
spelling	paper:paper_03037207_v221_n1-2_p9_Bussmann2023-06-08T15:29:03Z Activin and follistatin in rat mammary gland Bussmann, Ursula A. Lanuza, Guillermo Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types. In the present study, we investigated the role of activin in rat mammary gland on different stages of development. We found that activin A in vitro inhibits the proliferation of isolated acini, and this effect increases with the development of the gland. This factor also produces in vitro an inhibition of the final differentiation of acini obtained from 19th day pregnant rats. We also report the expression of activin receptors IIA and IIB mRNA in whole rat mammary gland and acini, with decreased levels of expression of type IIA (in both compartments) and IIB (in acini) during pregnancy and lactogenesis. In addition, we show that activin βB-subunit mRNA decreases throughout pregnancy, and that the mRNA levels of follistatin (Fst) (its ligand protein) are high in cycling rats and at the beginning of pregnancy and diminish thereafter, having the acini higher levels of expression. Our data show that activin βB-subunit, follistatin and ActRIIA and IIB transcripts are expressed in rat mammary gland at appropriate times and locations during development, allowing an interplay that might regulate activin action on growth and differentiation of the gland. © 2004 Elsevier Ireland Ltd. All rights reserved. Fil:Bussmann, U.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v221_n1-2_p9_Bussmann http://hdl.handle.net/20.500.12110/paper_03037207_v221_n1-2_p9_Bussmann
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta
spellingShingle	Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta Bussmann, Ursula A. Lanuza, Guillermo Activin and follistatin in rat mammary gland
topic_facet	Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta
description	Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types. In the present study, we investigated the role of activin in rat mammary gland on different stages of development. We found that activin A in vitro inhibits the proliferation of isolated acini, and this effect increases with the development of the gland. This factor also produces in vitro an inhibition of the final differentiation of acini obtained from 19th day pregnant rats. We also report the expression of activin receptors IIA and IIB mRNA in whole rat mammary gland and acini, with decreased levels of expression of type IIA (in both compartments) and IIB (in acini) during pregnancy and lactogenesis. In addition, we show that activin βB-subunit mRNA decreases throughout pregnancy, and that the mRNA levels of follistatin (Fst) (its ligand protein) are high in cycling rats and at the beginning of pregnancy and diminish thereafter, having the acini higher levels of expression. Our data show that activin βB-subunit, follistatin and ActRIIA and IIB transcripts are expressed in rat mammary gland at appropriate times and locations during development, allowing an interplay that might regulate activin action on growth and differentiation of the gland. © 2004 Elsevier Ireland Ltd. All rights reserved.
author	Bussmann, Ursula A. Lanuza, Guillermo
author_facet	Bussmann, Ursula A. Lanuza, Guillermo
author_sort	Bussmann, Ursula A.
title	Activin and follistatin in rat mammary gland
title_short	Activin and follistatin in rat mammary gland
title_full	Activin and follistatin in rat mammary gland
title_fullStr	Activin and follistatin in rat mammary gland
title_full_unstemmed	Activin and follistatin in rat mammary gland
title_sort	activin and follistatin in rat mammary gland
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v221_n1-2_p9_Bussmann http://hdl.handle.net/20.500.12110/paper_03037207_v221_n1-2_p9_Bussmann
work_keys_str_mv	AT bussmannursulaa activinandfollistatininratmammarygland AT lanuzaguillermo activinandfollistatininratmammarygland
_version_	1768545555398524928

Activin and follistatin in rat mammary gland

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