The organ culture of human epididymal tubules and their response to androgens
Human epididymal tubules from 9 patients undergoing orchidectomy were cultured for periods of up to 8 days with preservation of the histological structure of the tissue. Addition of androgens (testosterone, 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol) significantly increased the epithelial...
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1981
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v21_n3_p233_Tezon http://hdl.handle.net/20.500.12110/paper_03037207_v21_n3_p233_Tezon |
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paper:paper_03037207_v21_n3_p233_Tezon |
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paper:paper_03037207_v21_n3_p233_Tezon2023-06-08T15:29:03Z The organ culture of human epididymal tubules and their response to androgens androgen stimulation human epididymis protein synthesis amino acid androgen androstane 3,17 diol androstanediol androstanolone cyproterone cyproterone acetate drug derivative male contraceptive agent testosterone thymidine androgen androgen receptor androstanediol androstanolone cyproterone acetate radioisotope testosterone adult aged article drug effect epididymis human male metabolism organ culture amino acid h 3 dna replication endocrine system epididymis human cell in vitro study kidney tubule male genital system normal human protein synthesis thymidine h 3 Adult Aged Amino Acids Androgens Androstane-3,17-diol Contraceptive Agents, Male Cyproterone Cyproterone Acetate Epididymis Human Male Middle Age Organ Culture Stanolone Support, Non-U.S. Gov't Testosterone Thymidine Human epididymal tubules from 9 patients undergoing orchidectomy were cultured for periods of up to 8 days with preservation of the histological structure of the tissue. Addition of androgens (testosterone, 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol) significantly increased the epithelial height, the incorporation of [3H]amino acids and [3H]thymidine. The effect on protein synthesis was significant 48 h after the onset of treatment and was maximal after 3 days. The effect on DNA replication was maximal during the 3rd day of treatment. In both instances maximal activity was achieved at a concentration of 10-7 M of testosterone. Cyproterone acetate (10-5 M) was able to negate all effects of androgens. © 1981. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v21_n3_p233_Tezon http://hdl.handle.net/20.500.12110/paper_03037207_v21_n3_p233_Tezon |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
androgen stimulation human epididymis protein synthesis amino acid androgen androstane 3,17 diol androstanediol androstanolone cyproterone cyproterone acetate drug derivative male contraceptive agent testosterone thymidine androgen androgen receptor androstanediol androstanolone cyproterone acetate radioisotope testosterone adult aged article drug effect epididymis human male metabolism organ culture amino acid h 3 dna replication endocrine system epididymis human cell in vitro study kidney tubule male genital system normal human protein synthesis thymidine h 3 Adult Aged Amino Acids Androgens Androstane-3,17-diol Contraceptive Agents, Male Cyproterone Cyproterone Acetate Epididymis Human Male Middle Age Organ Culture Stanolone Support, Non-U.S. Gov't Testosterone Thymidine |
| spellingShingle |
androgen stimulation human epididymis protein synthesis amino acid androgen androstane 3,17 diol androstanediol androstanolone cyproterone cyproterone acetate drug derivative male contraceptive agent testosterone thymidine androgen androgen receptor androstanediol androstanolone cyproterone acetate radioisotope testosterone adult aged article drug effect epididymis human male metabolism organ culture amino acid h 3 dna replication endocrine system epididymis human cell in vitro study kidney tubule male genital system normal human protein synthesis thymidine h 3 Adult Aged Amino Acids Androgens Androstane-3,17-diol Contraceptive Agents, Male Cyproterone Cyproterone Acetate Epididymis Human Male Middle Age Organ Culture Stanolone Support, Non-U.S. Gov't Testosterone Thymidine The organ culture of human epididymal tubules and their response to androgens |
| topic_facet |
androgen stimulation human epididymis protein synthesis amino acid androgen androstane 3,17 diol androstanediol androstanolone cyproterone cyproterone acetate drug derivative male contraceptive agent testosterone thymidine androgen androgen receptor androstanediol androstanolone cyproterone acetate radioisotope testosterone adult aged article drug effect epididymis human male metabolism organ culture amino acid h 3 dna replication endocrine system epididymis human cell in vitro study kidney tubule male genital system normal human protein synthesis thymidine h 3 Adult Aged Amino Acids Androgens Androstane-3,17-diol Contraceptive Agents, Male Cyproterone Cyproterone Acetate Epididymis Human Male Middle Age Organ Culture Stanolone Support, Non-U.S. Gov't Testosterone Thymidine |
| description |
Human epididymal tubules from 9 patients undergoing orchidectomy were cultured for periods of up to 8 days with preservation of the histological structure of the tissue. Addition of androgens (testosterone, 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol) significantly increased the epithelial height, the incorporation of [3H]amino acids and [3H]thymidine. The effect on protein synthesis was significant 48 h after the onset of treatment and was maximal after 3 days. The effect on DNA replication was maximal during the 3rd day of treatment. In both instances maximal activity was achieved at a concentration of 10-7 M of testosterone. Cyproterone acetate (10-5 M) was able to negate all effects of androgens. © 1981. |
| title |
The organ culture of human epididymal tubules and their response to androgens |
| title_short |
The organ culture of human epididymal tubules and their response to androgens |
| title_full |
The organ culture of human epididymal tubules and their response to androgens |
| title_fullStr |
The organ culture of human epididymal tubules and their response to androgens |
| title_full_unstemmed |
The organ culture of human epididymal tubules and their response to androgens |
| title_sort |
organ culture of human epididymal tubules and their response to androgens |
| publishDate |
1981 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v21_n3_p233_Tezon http://hdl.handle.net/20.500.12110/paper_03037207_v21_n3_p233_Tezon |
| _version_ |
1768546208836485120 |